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To determine the intracellular signaling of keloid fibroblasts; especially the responses of purinoceptors

Research Project

Project/Area Number 23792052
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plastic surgery
Research InstitutionIwate Medical University

Principal Investigator

KURODA Takashi  岩手医科大学, 医学部, 助教 (30530224)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsATP / 肥厚性瘢痕 / ケロイド / 細胞内カルシウム濃度 / P2受容体 / 共焦点レーザー顕微鏡
Research Abstract

The purpose of the research is to determine the difference between intracellular signaling of normal fibroblasts and keloid fibroblasts, with emphasis on the responses of adenosine triphosphate (ATP) and purinoceptors. Fibroblasts were isolated from keloid and normal skin tissue that were obtained from human skin. Each cell has the specific responses of extracellular ATP. Fluorescent staining showed that each cell has the specific arrangement of cytoskeleton (F-actin) and endoplasmic reticulum density.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2010 2009 2008 2007 2006 2005

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (2 results) Book (1 results)

  • [Journal Article] Complementary actions of inhibitors of angiopoietin-2 and VE GF on tumor antiogenesis and growth2010

    • Author(s)
      Hashizume H, Falcon BL, Kuroda T, Baluk P, Coxon A, Yu D, Bready JV, Oliver JD, McDonald DM
    • Journal Title

      Cancer Res

      Volume: 70(6) Pages: 2213-23

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical localization of protease -activated receptors in cerebral and testicular arterioles of rats: their dependence on arteriole size and organ-specificity2008

    • Author(s)
      Misaki T, Satoh Y, Saino T, Kuroda T , Masu K, Russa DA, Ogawa A
    • Journal Title

      Arch Histol Cytol

      Volume: 71(3) Pages: 179-184

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of tomato lectin and phalloidin in morphological analyses of the vascular network of various tissues: With special reference to postnatal development of skin2007

    • Author(s)
      Kuroda T and Saino T
    • Journal Title

      J Iwate Med Assoc

      Volume: 59(2) Pages: 111-126

    • NAID

      110006274603

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 双葉皮弁を工夫した外鼻部分欠損の再建2006

    • Author(s)
      本多孝之、小林誠一郎、工藤信、黒田敬
    • Journal Title

      形成外科

      Volume: 49(7) Pages: 763-768

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative analysis of photodamage during fluorescence bioimaging: monitoring of nitric oxide production using DAF -2006

    • Author(s)
      Kuroda T, Satoh Y, Akutsu H, Shikanai Y, Miyata S, Saino T, Russa DA, Habara Y, Cui ZJ
    • Journal Title

      Bioimages

      Volume: 14 Pages: 9-18

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stress fracture of the clavicle associated with sternocostoclavicular hyperostosis2005

    • Author(s)
      Kuroda T, Ehara S, Murakami
    • Journal Title

      Skeletal Radiol

      Volume: 34 Pages: 424-426

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 高濃度ATP環境下で培養した線維芽細胞のATPに対する細胞内カルシウム動態の解析2010

    • Author(s)
      黒田敬、柏克彦、小林誠一郎、佐藤洋一
    • Organizer
      第115回日本解剖学会総会・全国学術集会
    • Place of Presentation
      盛岡
    • Related Report
      2012 Final Research Report
  • [Presentation] 高濃度ATP環境下での線維芽細胞の細胞特性の変化2010

    • Author(s)
      黒田敬、柏克彦、佐藤洋一、小林誠一郎
    • Organizer
      第19回日本形成外科学会基礎学術集会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Book] カルシウムイメジング法入門組織細胞化学2009

    • Author(s)
      佐藤洋一、斎野朝幸、黒田敬
    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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