To determine the intracellular signaling of keloid fibroblasts; especially the responses of purinoceptors

• Project/Area Number: 23792052
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Iwate Medical University
• Principal Investigator: KURODA Takashi 岩手医科大学, 医学部, 助教 (30530224)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ATP / 肥厚性瘢痕 / ケロイド / 細胞内カルシウム濃度 / P2受容体 / 共焦点レーザー顕微鏡

Research Abstract

The purpose of the research is to determine the difference between intracellular signaling of normal fibroblasts and keloid fibroblasts, with emphasis on the responses of adenosine triphosphate (ATP) and purinoceptors. Fibroblasts were isolated from keloid and normal skin tissue that were obtained from human skin. Each cell has the specific responses of extracellular ATP. Fluorescent staining showed that each cell has the specific arrangement of cytoskeleton (F-actin) and endoplasmic reticulum density.

Research Products

• [Journal Article] Complementary actions of inhibitors of angiopoietin-2 and VE GF on tumor antiogenesis and growth (2010)
  • Author(s): Hashizume H, Falcon BL, Kuroda T, Baluk P, Coxon A, Yu D, Bready JV, Oliver JD, McDonald DM
  • Journal Title: Cancer Res Volume: 70(6) Pages: 2213-23
  • Peer Reviewed
• [Journal Article] Immunohistochemical localization of protease -activated receptors in cerebral and testicular arterioles of rats: their dependence on arteriole size and organ-specificity (2008)
  • Author(s): Misaki T, Satoh Y, Saino T, Kuroda T , Masu K, Russa DA, Ogawa A
  • Journal Title: Arch Histol Cytol Volume: 71(3) Pages: 179-184
  • Peer Reviewed
• [Journal Article] Application of tomato lectin and phalloidin in morphological analyses of the vascular network of various tissues: With special reference to postnatal development of skin (2007)
  • Author(s): Kuroda T and Saino T
  • Journal Title: J Iwate Med Assoc Volume: 59(2) Pages: 111-126
  • NAID: 110006274603
  • Peer Reviewed
• [Journal Article] 双葉皮弁を工夫した外鼻部分欠損の再建 (2006)
  • Author(s): 本多孝之、小林誠一郎、工藤信、黒田敬
  • Journal Title: 形成外科 Volume: 49(7) Pages: 763-768
  • Peer Reviewed
• [Journal Article] Quantitative analysis of photodamage during fluorescence bioimaging: monitoring of nitric oxide production using DAF - (2006)
  • Author(s): Kuroda T, Satoh Y, Akutsu H, Shikanai Y, Miyata S, Saino T, Russa DA, Habara Y, Cui ZJ
  • Journal Title: Bioimages Volume: 14 Pages: 9-18
  • Peer Reviewed
• [Journal Article] Stress fracture of the clavicle associated with sternocostoclavicular hyperostosis (2005)
  • Author(s): Kuroda T, Ehara S, Murakami
  • Journal Title: Skeletal Radiol Volume: 34 Pages: 424-426
  • Peer Reviewed
• [Presentation] 高濃度ATP環境下で培養した線維芽細胞のATPに対する細胞内カルシウム動態の解析 (2010)
  • Author(s): 黒田敬、柏克彦、小林誠一郎、佐藤洋一
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] 高濃度ATP環境下での線維芽細胞の細胞特性の変化 (2010)
  • Author(s): 黒田敬、柏克彦、佐藤洋一、小林誠一郎
  • Organizer: 第19回日本形成外科学会基礎学術集会
  • Place of Presentation: 横浜
• [Book] カルシウムイメジング法入門組織細胞化学 (2009)
  • Author(s): 佐藤洋一、斎野朝幸、黒田敬