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Analysis of infection control on endodontic treatment using genetic engineering method

Research Project

Project/Area Number 23792172
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

NAGAYAMA Tomotaka  大阪大学, 大学院・歯学研究科, 招聘教員 (60456944)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords歯学 / 歯内療法学 / 細菌 / バイオフィルム / 術中感染 / ラバーダム防湿 / 16S rRNA遺伝子解析法 / 歯内療法 / 16s rRNA遺伝子解析法 / 難治性根尖性歯周炎
Research Abstract

To clarify the precise condition of infection control on endodontic treatment, bacterial biofilm in root canal before or after treatment, which was classified into 4 groups (pulpectomy or infected root canal treatment, with or without rubber dam isolation technique), was analyzed by PCR-based 16S rRNA gene assay. Furthermore, patient’s saliva, dental plaque and glove was analyzed similarly. Although it was difficult to clarity the route of infection completely, we demonstrated the usefulness of rubber dam isolation technique for infection control on endodontic treatment.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (7 results)

All 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (5 results)

  • [Journal Article] う蝕象牙質除去後の残存細菌に Er:YAG レーザーが与える影響2013

    • Author(s)
      高橋雄介,吉岡靖介,朝日陽子,永山智崇,野杁由一郎,林美加子
    • Journal Title

      日歯科保誌

      Volume: 56(1) Pages: 1-8

    • NAID

      110009596775

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] う蝕象牙質除去後の残存細菌にEr:YAGレーザーが与える影響2013

    • Author(s)
      高橋雄介
    • Journal Title

      日本歯科保存学会誌

      Volume: 56(1) Pages: 1-8

    • Related Report
      2012 Annual Research Report
  • [Presentation] 根尖孔外バイオフィルムモデルにおけるラット根尖病巣のマイクロ CT 解析2012

    • Author(s)
      呉本勝隆,野杁由一郎,永山智崇,騎馬和歌子,前薗葉月,山本れいこ,米田直道,林美加子,恵比須繁之,石本卓也,中野貴由
    • Organizer
      第137回秋季日本歯科保存学会
    • Place of Presentation
      広島
    • Year and Date
      2012-11-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 難治性根尖性歯周炎に関わるバイオフィルム構成細菌種と臨床症状との関係2012

    • Author(s)
      藪根敏晃,野杁由一郎,山本れいこ,山口幹代,朝日陽子,前薗葉月,永山智崇,呉本勝隆,騎馬和歌子,林美加子,恵比須繁之
    • Organizer
      第136回春季日本歯科保存学会
    • Place of Presentation
      沖縄
    • Year and Date
      2012-06-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 軟化象牙質除去後の残存細菌に対してEr:YAG レーザーが与える影響2012

    • Author(s)
      吉岡靖介,野杁由一郎,高橋雄介,藪根敏晃,朝日陽子,永山智崇,古谷優,栗本絵里子,北川蘭奈,武田侑子,大嶋淳,山本由美子,住岡龍一,永井真澄,板東秀典,林美加子
    • Organizer
      第136回春季日本歯科保存学会
    • Place of Presentation
      沖縄
    • Year and Date
      2012-06-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 難治性根尖性歯周炎に関わるバイオフィルム構成細菌種と臨床症状との関係

    • Author(s)
      薮根敏晃
    • Organizer
      日本歯科保存学会 2012年度春季学術大会(第136回)
    • Place of Presentation
      宜野湾市 沖縄コンベンションセンター
    • Related Report
      2012 Annual Research Report
  • [Presentation] 根尖孔外バイオフィルムモデルにおけるラット根尖病巣のマイクロCT解析

    • Author(s)
      呉本勝隆
    • Organizer
      日本歯科保存学会 2012年度秋季学術大会(第137回)
    • Place of Presentation
      広島市 広島国際会議場
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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