| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
To maintain normal function, the lymphatic endothelium is regulated by cell-cell junctions. One of objectives of this study was to characterize cell junctions in lymphatic endothelial cells and to investigate the effects of the inflammatory cytokine TNF-α on altered cell-cell junctions.
Cultured human dermal lymphatic endothelial cells (HDLEC) were immunostained with the tight junction marker, and adherens junction markers, VE-cadherin. In TNF-α-treated HDLEC, we evaluated changes in endothelial cell junctions by immunostaining and through the use of transendothelial electrical resistance (TER).
The results revealed a heterogeneous distribution of cell junctions in HDLEC involving continuous and discontinuous junctions. Our data also suggest that TNF-α alters the normal distribution of cell junctions and affects the endothelial barrier of cultured lymphatic endothelial cells.
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