Characterization of junctional complex of lymphatic endothelial cells in caner lymphangiogenesis
Project/Area Number |
23792340
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Surgical dentistry
|
Research Institution | Kobe University |
Principal Investigator |
AKASHI Masaya 神戸大学, 医学部附属病院, 助教 (40597168)
|
Project Period (FY) |
2011-04-28 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | 口腔癌 / リンパ管内皮細胞 / 細胞間接着複合体 / 炎症性サイトカイン / 国際情報交換 / 癌 / リンパ管 / 細胞間接着 / 内皮細胞 |
Outline of Final Research Achievements |
To maintain normal function, the lymphatic endothelium is regulated by cell-cell junctions. One of objectives of this study was to characterize cell junctions in lymphatic endothelial cells and to investigate the effects of the inflammatory cytokine TNF-α on altered cell-cell junctions. Cultured human dermal lymphatic endothelial cells (HDLEC) were immunostained with the tight junction marker, and adherens junction markers, VE-cadherin. In TNF-α-treated HDLEC, we evaluated changes in endothelial cell junctions by immunostaining and through the use of transendothelial electrical resistance (TER). The results revealed a heterogeneous distribution of cell junctions in HDLEC involving continuous and discontinuous junctions. Our data also suggest that TNF-α alters the normal distribution of cell junctions and affects the endothelial barrier of cultured lymphatic endothelial cells.
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Report
(5 results)
Research Products
(4 results)