Investigation of oral mucosal inflammatory disease developing mechanism via Toll-like receptor

• Project/Area Number: 23792346
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: NISHI Hiromi 広島大学, 大学病院, 助教 (70403558)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 口腔粘膜炎症性疾患 / 口腔粘膜上皮細胞 / 歯肉線維芽細胞 / Toll-like receptor / 口腔粘膜 / ケモカイン / 口腔粘膜細胞 / 炎症性口腔粘膜疾患

Research Abstract

Oral keratinocytes and fibroblasts may augment host defense and inflammatory responses against oral microorganisms invasion. However, it remains unclear whether oral keratinocytes and fibroblasts produce specific inflammatory proteins in response to Pathogen-associated molecular patterns (PAMPS) and associated with oral mucosal inflammatory diseases. Our results showed that oral keratinocytes and fibroblasts expressed mRNA of TLRs 1-10. IL-8 and CXCL10 production from oral keratinocytes and fibroblasts was induced by treatment with various TLR agonisits. These findings suggest that recognition of microorganism-derived pathogen in oral keratinocytes and fibroblasts by TLR may have important role to orchestrate host immune responses and promoting hyper/hypo reactions related to oral inflammatory diseases via production of various chemokines.

Research Products

• [Journal Article] Itraconazole inhibits TNF-α-induced CXCL10 expression in oral fibroblasts (2014)
  • Author(s): Ohta K, Ishida Y, Fukui A, Nishi H, Naruse T, Takechi M, Kamata N
  • Journal Title: Oral Dis Volume: 21 Issue: 1 Pages: 106-112
  • DOI: 10.1111/odi.12226
  • Peer Reviewed
• [Journal Article] Interleukin-8 and CXCL10 expressions in oral keratinocytes and fibroblasts via Toll-like receptors (2013)
  • Author(s): Fukui A, Ohta K, Nishi H, Shigeishi H, Tobiume K, Takechi M, Kamata N
  • Journal Title: Microbiol Immunol Volume: 57 Pages: 198-206
  • NAID: 40019615142
  • Peer Reviewed
• [Journal Article] Interleukin-8 and CXCL10 expression in oral keratinocytes and fibroblasts via Toll-like receptors. (2013)
  • Author(s): Fukui A, Ohta K, Nishi H, Shigeishi H, Tobiume K, Takechi M, Kamata N.
  • Journal Title: Microbiol Immunol Volume: 57(3) Issue: 3 Pages: 198-206
  • DOI: 10.1111/1348-0421.12022
  • Peer Reviewed
• [Journal Article] Toll-like receptor (TLR) expression and TLR-mediated IL-8 production by human submandibular gland epithelial cells
  • Author(s): Ohta K, Ishida Y, Fukui A, Mizuta K, Nishi H, Takechi M, Kamata
  • Journal Title: N Mol Med Rep Volume: (accepted)
  • Peer Reviewed
• [Presentation] 口腔粘膜上皮細胞におけるCandida albicansβ-glucanによる酸化ストレスとHemeoxygenase-1の発現誘導 (2013)
  • Author(s): 石田陽子,太田耕司,福井暁子,西裕美,奥井岳,鳴瀬貴子,武知正晃,鎌田伸之
  • Organizer: 日本口腔組織培養学会設立50周年記念大会・総会
  • Place of Presentation: 東京
  • Year and Date: 2013-11-23
• [Presentation] 口腔粘膜上皮細胞におけるCandida albicansβ-glucanによるHemeoxygenase-1の発現誘導 (2013)
  • Author(s): 石田陽子,太田耕司,福井暁子,西裕美,奥井岳,鳴瀬貴子,武知正晃,鎌田伸之
  • Organizer: 第58回日本口腔外科学会総会・学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2013-10-12
• [Presentation] 口腔粘膜上皮細胞におけるCandida albicansβ-glucanによるHemeoxygenase-1の発現誘導と酸化ストレスに対する防御応答 (2013)
  • Author(s): 石田陽子,太田耕司,福井暁子,西裕美,奥井岳,鳴瀬貴子,武知正晃,鎌田伸之
  • Organizer: 第23回口腔内科学会・第26回口腔診断学会合同学術大会
  • Place of Presentation: 東京
  • Year and Date: 2013-09-13
• [Presentation] 口腔粘膜上皮細胞におけるCandida albicansによるHemeoxygenase-1の発現誘導 (2013)
  • Author(s): 石田陽子,太田耕司,福井暁子,西裕美,奥井岳,鳴瀬貴子,武知正晃,鎌田伸之
  • Organizer: 第67回NPO法人日本口腔科学会学術大会
  • Place of Presentation: 宇都宮
  • Year and Date: 2013-05-22
• [Presentation] 歯肉線維芽細胞における炎症性ケモカイン発現誘導に対するアゾール系抗真菌剤イトコナゾールの抑制作用の検討 (2012)
  • Author(s): 石田陽子,太田耕司,福井暁子,西裕美,武知正晃,鎌田伸之
  • Organizer: 第49回日本口腔組織培養学会学術大会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-17
• [Presentation] 歯肉線維芽細胞の炎症性ケモカイン発現誘導に対するイトコナゾールの抑制作用の検討 (2012)
  • Author(s): 太田耕司,福井暁子,西裕美,石田陽子,武知正晃,鎌田伸之
  • Organizer: 第22回日本口腔粘膜学会合同学術大会
  • Place of Presentation: 東京
  • Year and Date: 2012-09-20
• [Presentation] 口腔粘膜上皮細胞,線維芽細胞における抗真菌剤イトコナゾールの抗炎症作用の検討 (2012)
  • Author(s): 太田耕司,福井暁子,西裕美,石田陽子,武知正晃,鎌田伸之
  • Organizer: 第66回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 広島
  • Year and Date: 2012-05-17
• [Presentation] 口腔粘膜上皮細胞,線維芽細胞におけるRIG-Iシグナルの機能解析 (2011)
  • Author(s): 福井暁子,太田耕司,重石英生,西裕美,武知正晃,鎌田伸之
  • Organizer: 第56回日本口腔外科学会総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-22
• [Presentation] Expression and function of Retionic acid-inducible gene-I (RIG-I) in oral keratinocyte and fibroblast (2011)
  • Author(s): Fukui A, Ohta K, Shigeishi H, Nishi H, Takechi M, Kamata N
  • Organizer: 4^<th> Hiroshima Conference on Education and science in Dentistry
  • Place of Presentation: Hiroshima
  • Year and Date: 2011-10-10
• [Presentation] 口腔粘膜上皮細胞,歯肉線維芽細胞におけるToll-like receptorを介した炎症性ケモカインの発現誘導 (2011)
  • Author(s): 太田耕司,福井暁子,西裕美,重石英生,武知正晃,鎌田伸之
  • Organizer: 第21回日本口腔粘膜学会総会・学術大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2011-09-24
• [Presentation] 口腔粘膜上皮細胞,歯肉線維芽細胞におけるRIG-Iの発現とシグナル伝達経路の機能解析 (2011)
  • Author(s): 福井暁子,太田耕司,重石英生,西裕美,武知正晃,鎌田伸之
  • Organizer: 第21回日本口腔粘膜学会総会・学術大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2011-09-24
• [Presentation] 口腔粘膜上皮細胞,歯肉線維芽細胞におけるRIG-Iを介した炎症性ケモカインの発現とシグナル伝達経路の解析 (2011)
  • Author(s): 福井暁子,太田耕司,重石英生,西裕美,武知正晃,鎌田伸之
  • Organizer: 第65回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-21