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New strategy for bone regeneration based on RNA silencing of GPCR-B

Research Project

Project/Area Number 23792421
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthodontic/Pediatric dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

WATARI IPPEI  東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (10431941)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsGPCR class B / GLP-1 / GIP / 骨再生 / RNAサイレンシング / 唾液腺遺伝子治療 / GPCR / インクレチン / 骨芽細胞 / GPCR-B / 骨代謝 / 歯槽骨再生 / 耐糖能異常 / RNAi / non-coding RNA
Research Abstract

The purpose of this project is to explore the possible implementation of GPCR-B signaling in a new treatment method for bone diseases using an in vitro approach. Incretin receptors, GLP-1 receptor (GLP-1R) and GIP receptor (GIPR), belong to GPCR-B family and are suggested to play an important role in bone metabolism. The osteoblastic cell line, MC3T3-E1 was found to express both GIPR and GLP-1R. The quantitative PCR analysis showed that expression of both receptors was upregulated after stimulation with BMP-2. Determination of molecular mechanisms involved in regulation of bone metabolism by incretin receptors-related signaling will help to developed effective strategy for bone regeneration.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (29 results)

All 2014 2013 2012 Other

All Journal Article (12 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results) Presentation (14 results) (of which Invited: 3 results) Book (2 results) Remarks (1 results)

  • [Journal Article] The influence of gestational diabetes in craniofacial growth of newborn2014

    • Author(s)
      Ippei Watari, Jutiporn Privatananupunt, Hsu Jui-Chin, Katarzyna Anna Podyma-Inoue, Takashi Ono
    • Journal Title

      J Soc Wom Health Sci Res

      Volume: 3(1) Pages: 57-62

    • Related Report
      2013 Final Research Report
  • [Journal Article] Degeneration of fungiform and circumvallate papillae following molar extraction in rats2014

    • Author(s)
      Hsu JC, Watari I, Ono R, Privatananupunt J, Mizumachi-Kubono M, Honda K, Ishida Y, Ono T
    • Journal Title

      Acta Odontol Scand

      Volume: (In press)

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/?term=Hsu+HC+Watari+Ippei+2014

    • Related Report
      2013 Final Research Report
  • [Journal Article] Expression of glucagon-like peptide-1 receptor and glucose-dependent insulinotropic polypeptide receptor is regulated by the glucose concentration in mouse osteoblastic MC3T3-E1 cells2014

    • Author(s)
      Emina Aoyama, Ippei Watari, Katarzyna Anna Podyma-Inoue, Masaki Yanagishita, Takashi Ono
    • Journal Title

      Int J Mol Med

      Volume: (In press) Issue: 2 Pages: 475-82

    • DOI

      10.3892/ijmm.2014.1787

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of glucose-dependent insulinotropic polypeptide and its receptor in the rat major salivary glands2014

    • Author(s)
      Jutiporn Privatananupunt, Ippei Watari, Katarzyna Anna Podyma-Inoue, Mariko Kubono, Takashi Ono
    • Journal Title

      Acta Histochem

      Volume: 116(4) Issue: 4 Pages: 545-550

    • DOI

      10.1016/j.acthis.2013.11.007

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of glucagon-like peptide-1 receptor and glucose-dependent insulinotropic polypeptide receptor is regulated by the glucose concentration in mouse osteoblastic MC3T3-E1 cells.2014

    • Author(s)
      Emina Aoyama, Ippei Watari, Katarzyna Anna Podyma-Inoue, Masaki Yanagishita, Takashi Ono
    • Journal Title

      International Journal of Molecular Medicine

      Volume: -

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 妊娠中の耐糖能低下が母体の口腔内および新生児の頭蓋顎顔面成長発育に与える影響2014

    • Author(s)
      渡一平、パイワッタナヌパン ジュティポーン、許瑞瑾、井上カタジナアンナ、青山絵美奈、小野卓史
    • Journal Title

      女性健康科学研究会誌

      Volume: 3 (1) Pages: 57-62

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Expression of glucose-dependent insulinotropic polypeptide and its receptor in the rat major salivary glands.2014

    • Author(s)
      Jutiporn Privatananupunt, Ippei Watari, Katarzyna Anna Podyma-Inoue, Mariko Kubono, Takashi Ono.
    • Journal Title

      Acta Histochemica

      Volume: -

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unilateral maxillary molar extraction influences AQP5 expression and distribution in the rat submandibular salivary gland2012

    • Author(s)
      Mariko Mizumachi-Kubono, Ippei Watari, Yuji Ishida, Takashi Ono
    • Journal Title

      Arch Oral Biol

      Volume: 57 Issue: 7 Pages: 877-883

    • DOI

      10.1016/j.archoralbio.2012.02.006

    • Related Report
      2013 Final Research Report
  • [Journal Article] Effect of functional shift of the mandible on lubrication of the temporomandibular joint.2012

    • Author(s)
      Ikuko Kure-Hattori,Ippei Watari,Maki Takei,Yuji Ishida,Ikuo Yonemitsu,Takashi Ono.
    • Journal Title

      Archives of Oral Biology

      Volume: Jul;57(7) Issue: 7 Pages: 987-94

    • DOI

      10.1016/j.archoralbio.2012.01.006

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unilateral maxillary molar extraction influences AQP5 expression and distribution in the rat submandibular salivary gland.2012

    • Author(s)
      Mizumachi-Kubono M., Watari I., Ishida U., Ono T
    • Journal Title

      Arch Oral Biol.

      Volume: 57 Pages: 877-883

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of functional shift of the mandible on lubrication of the temporomandibular joint.2012

    • Author(s)
      Kure-Hattori I, Watari I, Ishida Y, Yonemitsu I, Ono T.
    • Journal Title

      Arch Oral Biol.

      Volume: 57 Pages: 987-994

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Unilateral maxillary molar extraction influences AQP5 expression and distribution in the rat submandibular salivary gland.2012

    • Author(s)
      Mariko Mizumachi-Kubono, Ippei Watari, Yuji Ishida, Takashi Ono
    • Journal Title

      Archives of Oral Biology

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] MC3T3-E1細胞におけるグルコース濃度依存性のインクレチン受容体の発現変化2013

    • Author(s)
      青山絵美奈, 渡一平, 井上カタジナアンナ, 柳下正樹, 小野卓史
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 妊娠中の耐糖能低下が母体の口腔内および新生児の頭蓋顎顔面成長発育に及ぼす影響について2013

    • Author(s)
      渡一平
    • Organizer
      第4回女性健康科学研究会
    • Place of Presentation
      大阪
    • Year and Date
      2013-05-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Unilateral nasal obstruction affects the distrubution of fungiform papillae in the rat tongue2013

    • Author(s)
      Hsu JC., Watari I., Honda K., Mizumachi-Kubono M., Kure-Hattori I., Ishida Y., Ono T
    • Organizer
      第72回日本矯正歯科学会大会
    • Place of Presentation
      松本
    • Related Report
      2013 Final Research Report
  • [Presentation] Glucose-dependent insulinotoropic polypeptide expression in the rat major salivary gland2013

    • Author(s)
      Jutiporn Privatananupunt, Ippei Watari, Podyma-Inoue KA, Mariko Kubono, Takashi Ono
    • Organizer
      The 2nd Meeting of th International Association of Dental Research-Asia Pacific Region (IADR-APR)
    • Place of Presentation
      Bang Kong
    • Related Report
      2013 Final Research Report
  • [Presentation] MC3T3-E1細胞におけるグルコース濃度によるインクレチン受容体の発現変化2013

    • Author(s)
      青山 絵美奈、渡 一平、井上 カタジナ アンナ、柳下 正樹、 小野 卓史
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      岡山 岡山コンベンションセンター
    • Related Report
      2013 Annual Research Report
  • [Presentation] 妊娠中の耐糖能低下が母体の口腔内および新生児の頭蓋顎顔面成長発育に及ぼす影響について2012

    • Author(s)
      渡一平, 井上カタジナアンナ, 服部育子, 窪野真理子, パイワッタナヌパンジュティポーン
    • Organizer
      第3回ロート製薬女性健康科学研究会
    • Place of Presentation
      東京
    • Year and Date
      2012-05-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Molar extraction influences the distribution of fungiform papillae in the rat tongue2012

    • Author(s)
      Hsu JC., Watari I., Honda K., Mizumachi-Kubono M., Kure-Hattori I., Ishida Y., Ono T
    • Organizer
      第71回日本矯正歯科学会大会
    • Place of Presentation
      盛岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 唾液腺におけるGLP-1R受容体の発現は咬合状態に影響される2012

    • Author(s)
      小野理恵子, 渡一平, 窪野真理子, 石田雄之, 服部育子, 本田康二, 小野卓史
    • Organizer
      第71回日本矯正歯科学会大会
    • Place of Presentation
      盛岡
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Considerations during orthodontic treatment2012

    • Author(s)
      Abbassy MA., Watari I., Ono T
    • Organizer
      3rd International Conference of Faculty of Dentistry at King Abdulaziz University
    • Place of Presentation
      Saudi Arabia
    • Related Report
      2013 Final Research Report
  • [Presentation] Diabetes Mellitus: Considerations during orthodontic treatment.2012

    • Author(s)
      Abassy MA., Watari I., Ono T.
    • Organizer
      3rd International Conference of Faculty of Dentistry at King Abdulaziz University
    • Place of Presentation
      Saudia Arabia
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Molar extraction influences the distribution of fungiform papillae in the rat tongue.2012

    • Author(s)
      Hsu JC., Watari I., Honda K., Mizumachi-Kubono M., Kure-Hattori I., Ishida Y., Ono T.
    • Organizer
      第71回日本矯正歯科学会大会
    • Place of Presentation
      盛岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Diabetes Mellitus: Consideration during orthodontic treatment2012

    • Author(s)
      M.A.Abbassy, I. Watari , T.Ono
    • Organizer
      3rd International Conference of Faculty of Dentistry at King Abdulaziz University
    • Place of Presentation
      Jeddah, Saudia Arabia
    • Related Report
      2011 Research-status Report
  • [Presentation] 妊娠中の耐糖能低下が母体の口腔内および新生児の頭蓋顎顔面成長発育に及ぼす影響について

    • Author(s)
      渡一平、パイワッタナヌパン ジュティポーン、許瑞瑾、井上カタジナアンナ、青山絵美奈、小野卓史
    • Organizer
      第4回 女性健康科学研究会
    • Place of Presentation
      大阪 グランフロント大阪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 妊娠中の耐糖能低下が母体の口腔内および新生児の頭蓋顎顔面成長発育に及ぼす影響について

    • Author(s)
      渡一平, 井上カタジナアンナ, 服部育子, 窪野真理子, パイワッタナヌパン ジュティポーン.
    • Organizer
      第3回ロート製薬女性健康科学研究会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] Type I Diabetes2013

    • Author(s)
      edited by Alan P. Escher, Alice Li
    • Publisher
      InTech
    • Related Report
      2013 Final Research Report
  • [Book] Type I Diabetes -Chapter 16 The Effect of Type I Diabetes Mellitus on the Dentofacial Complex2013

    • Author(s)
      Mona Aly Abbassy, Ippei Watari, Takashi Ono
    • Publisher
      INTECH
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/dent/ort1/ort1-J.htm

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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