| Project/Area Number |
23792459
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
HARADA Kyoko 大阪歯科大学, 歯学部, 准教授 (80434794)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 乳歯歯髄 |
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| Research Abstract |
In the present study, we examined whether TNF-alpha affected the production of MMP-2 in deciduous dental pulp fibroblasts and its signaling pathways utilizing gelatin zymography and western blotting analysis. TNF-alpha increased the expression of MMP-2 in a dose-dependent manner in deciduous dental pulp fibroblasts. LY294002 and Wortmannin, which are PI3-K inhibitors, inhibited the MMP-2 production induced by TNF-alpha in deciduous dental pulp fibroblasts. Moreover, in deciduous dental pulp fibroblasts cultured with TNF-alpha, AKT was phosphorylated in a time-dependent manner with the maximum phosphorylation at 30 min, and LY294002 and Wortmannin abolished this phosphorylation of AKT in TNF-alpha-stimulated deciduous dental pulp fibroblasts. These results suggest that TNF-alpha may enhance pulp tissue destruction during pulp inflammation in part by regulating MMP-2, and that the AKT pathway is involved in MMP-2 production in deciduous dental pulp fibroblasts.
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