The analysis of inflammatory responses and effective care in chronic wounds

• Project/Area Number: 23792534
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Fundamental nursing
• Research Institution: Tohoku University
• Principal Investigator: KANNO Emi 東北大学, 大学院・医学系研究科, 助教 (10431595)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 看護技術 / 創傷治癒 / 感染 / 炎症 / 免疫 / 創傷治癒学 / 慢性皮膚潰瘍 / 緑膿菌 / 難治性皮膚潰瘍

Research Abstract

Several bacteria, including Pseudomonas aeruginosa exist in chronic wounds with prolonged inflammatory reaction. In the present study, using a rat model with full-thickness dermal wounds, we examined the effect of P. aeruginosa and quorum sensing (QS) molecules, N-(3-oxododecanoyl)-_L-homoserine lactone (3-oxo-C_<12>-HSL), which is produced by P. aeruginosa. Within 3 days, inoculation with P. aeruginosa or3-oxo-C_<12>-HSL had accelerated local infiltration of neutrophils and tumor necrosis factor-α. These results indicate that 3-oxo-C_<12>-HSL may be involved in induction of inflammatory responses.

Research Products

• [Journal Article] Neutrophil-derived tumor necrosis factor-α contributes to acute wound healing promoted byN-(3-oxododecanoyl)-L-homoserine lactone from Pseudomonas aeruginosa (2013)
  • Author(s): Emi Kanno, Kazuyoshi Kawakami, Shinichi Miyairi, Hiromasa Tanno, Hirono Otomaru, Arina Hatanaka, Shiori Sato, Keiko Ishii, Denso Hayashi, Nobuhito Shibuya, Yoshimichi Imai, Naomasa Gotoh, Ryoko Maruyama and Masahiro Tachi
  • Journal Title: Journal of Dermatological Science Volume: 70 Pages: 130-138
  • URL: http://dx.doi.org/10.1016/j.jdermsci.2013.01.004
  • Peer Reviewed
• [Journal Article] Neutrophil-derived tumor necrosis factor-α contributes to acute wound healing promoted by N-(3-oxododecanoyl)-l-homoserine lactone from Pseudomonas aeruginosa. (2013)
  • Author(s): Kanno, E., Kawakami, K, Miyairi, S., Tanno, H., Otomaru, H., Hatanaka, A., Sato, S., Ishii, K., Hayashi, D., Shibuya, N., Imai, Y., Gotoh, N., Maruyama, R., and Tachi, M.
  • Journal Title: J. Dermatol. Sci. Volume: 70 Issue: 2 Pages: 130-138
  • DOI: 10.1016/j.jdermsci.2013.01.004
  • Peer Reviewed
• [Journal Article] 皮膚創傷治癒過程におけるbFGF産生へのTNF-αの関与 (2012)
  • Author(s): 菅野恵美,丹野寛大,館正弘
  • Journal Title: 日本褥瘡学会誌 Volume: 14(2) Pages: 113-120
  • Peer Reviewed
• [Journal Article] Wound healing in skin promoted by inoculation with Pseudomonas aeruginosa PAO1: the critical role of tumor necrosis factor-α secreted from infiltrating neutrophils (2011)
  • Author(s): Emi Kanno, Kazuyoshi Kawakami, Masae Ritsu, Keiko Ishii, Hiromasa Tanno, Souhachi Toriyabe, Yoshimichi Imai, Ryoko Maruyama and Masahiro Tachi
  • Journal Title: Wound Repair and Regeneration Volume: 19(5) Issue: 5 Pages: 608-621
  • DOI: 10.1111/j.1524-475x.2011.00721.x
  • Peer Reviewed
• [Presentation] Skin wound healing promoted by a quorum-sensing moleclue,N-(3-Oxododecanoyl)-_L-homoserine lactone from Pseudomonas aeruginosa (2012)
  • Author(s): Emi Kanno, Kazuyoshi Kawakami, Shinichi Miyairi, Hiromasa Tanno, Keiko Ishii, Denso Hayashi, Ryoko Maruyama, Masahiro Tachi
  • Organizer: 4th Congress of the World Union of Wound Healing Societies
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2012-09-26
• [Presentation] N-(3-oxododecanoyl)-L-homoserine lactone, a quorum-sensing moleculefrom Pseudomonas aeruginosa, promotesacute wound healing (2012)
  • Author(s): KANNO Emi, TANNO Hiromasa, ISHII Keiko, KAWAKAMI Kazuyoshi
  • Organizer: 第41回日本免疫 学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 緑膿菌クオラムセンシング分子N-butanoyl-L-homoserine lactoneが創傷治癒過程に与える影響 (2012)
  • Author(s): 菅野恵美,丹野寛大,川上和義,宮入伸一,石井恵子,丸山良子,館正弘
  • Organizer: 第42回日本創傷治癒学会
  • Place of Presentation: 札幌
• [Presentation] Skin wound healing and cytokine response promoted by a quorum-sensing moleclue from Pseudomonas aeruginosa (2012)
  • Author(s): Emi Kanno, Kazuyoshi Kawakami, Shinichi Miyairi, Hiromasa Tanno, Keiko Ishii, Denso Hayashi, Ryoko Maruyama, Masahiro Tachi
  • Organizer: IMMUNOLOGY
  • Place of Presentation: Boston, USA
• [Presentation] Skin wound healing promoted by a quorum-sensing moleclue, N-(3-Oxododecanoyl)-L-homoserine lactone from Pseudomonas aeruginosa (2012)
  • Author(s): Emi Kanno
  • Organizer: 4th Congress of the World Union of Wound Healing Societies
  • Place of Presentation: Yokohama, Japan
• [Presentation] Skin wound healing and cytokine response promoted by a quorum-sensing moleclue from Pseudomonas aeruginosa (2012)
  • Author(s): Emi Kanno
  • Organizer: IMMUNOLOGY 2013
  • Place of Presentation: Boston, USA
• [Presentation] N-(3-oxododecanoyl)-L-homoserine lactone, a quorum-sensing molecule from Pseudomonas aeruginosa, promotes acute wound healing (2012)
  • Author(s): KANNO Emi
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸市
• [Presentation] 緑膿菌クオラムセンシング分子N-butanoyl-L-homoserine lactoneが創傷治癒過程に与える影響 (2012)
  • Author(s): 菅野恵美
  • Organizer: 第42回日本創傷治癒学会
  • Place of Presentation: 札幌市
• [Presentation] 緑膿菌クオラムセンシング分子による創傷治癒の促進と創部炎症反応の誘導 (2011)
  • Author(s): 菅野恵美,川上和義,乙丸礼乃,石井恵子,丹野寛大,林殿聡,丸山良子,館正弘
  • Organizer: 第41回日本創傷治癒学会
  • Place of Presentation: 名古屋
• [Presentation] 緑膿菌クオラムセンシング機構が創傷治癒過程と創部炎症反応に与える影響 (2011)
  • Author(s): 菅野恵美, 川上和義, 乙丸礼乃, 石井恵子, 丹野寛大, 林殿聡, 丸山良子, 館正弘
  • Organizer: 第13回日本褥瘡学会学術集会
  • Place of Presentation: 福岡
• [Book] 第V章感染症2.慢性感染症Critical colonization.市岡滋監修,創傷のすべて (2012)
  • Author(s): 菅野恵美,館正弘
  • Publisher: 克誠堂出版
• [Book] ナースのためのアドバンスド創傷ケア (PartIIアドバンスド創傷ケアの実際 2. 実際編 創傷病態生理学: 各論 慢性創傷の病態学) (2012)
  • Author(s): 菅野恵美
  • Publisher: 照林社
• [Book] 創傷のすべて (第V章 感染症 2. 慢性感染症 Critical colonization.) (2012)
  • Author(s): 菅野恵美
  • Publisher: 克誠堂出版
• [Book] 創傷ケアの基礎知識と実践,第1章4.慢性創傷に関する知識 (2011)
  • Author(s): 菅野恵美,館正弘
  • Publisher: メディカ出版
• [Book] 創傷ケアの基礎知識と実践 第1章 創傷ケアに必要な基礎知識 4 慢性創傷に関する知識 (2011)
  • Author(s): 菅野恵美, 館正弘
  • Publisher: メディカ出版
• [Remarks]
  • URL: http://www.snp.med.tohoku.ac.jp/
• [Remarks] 東北大学大学院医学系研究科 看護アセスメント学分野
  • URL: http://www.snp.med.tohoku.ac.jp/