Analysis for endocannabinoid signaling in the epileptogenic circuit

• Project/Area Number: 23800001
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Hokkaido University
• Principal Investigator: UCHIGASHIMA Motokazu 北海道大学, 大学院・医学研究科, 助教 (10614662)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 内因性カンナビノイド / カンナビノイド受容体 CB1 / 歯状回 / てんかん / 神経科学 / 海馬 / シナプス

Research Abstract

This research project reveled the molecular anatomical basis fora major endocannabinoid, 2-arachidonoyl glycerol (2-AG), to effectively inhibit synaptic transmission at mossy cell-granule cell synapses, which constitute a potentiaepilectogenic circuit in the CNS. This suggests that 2-AG plays a role as a synapticcircuit breaker to prevent the epiletogenesis.

Research Products

• [Journal Article] Type 2 K+ -Cl- cotransporter ispreferentially recruited to climbingfiber synapses during development andthe stellate cell-targeting dendriticzone at adulthood in cerebellar Purkinje cells (2013)
  • Author(s): Kawakita I, Uchigashima M, Konno K, Miyazaki T, Yamasaki M, Watanabe M
  • Journal Title: Eur J Neurosci Volume: 37(4) Issue: 4 Pages: 532-43
  • DOI: 10.1111/ejn.12076
  • Peer Reviewed
• [Journal Article] Three types of neurochemical projection from the bed nucleus of the stria terminalis to the ventral tegmental area in adult mice. (2012)
  • Author(s): Kudo T, Uchigashima M, Miyazaki T, Konno K, Yamasaki Y, Yanagawa Y, Minami M, Watanabe M
  • Journal Title: J. Neurosci. Volume: 32 Issue: 50 Pages: 18035-18046
  • DOI: 10.1523/jneurosci.4057-12.2012
  • NAID: 120005295651
  • Peer Reviewed
• [Journal Article] Striatal indirect pathway contributes to selection accuracy of learned motor actions. (2012)
  • Author(s): Nishizawa K
  • Journal Title: J. Neurosci. Volume: 32 Issue: 39 Pages: 13421-13432
  • DOI: 10.1523/jneurosci.1969-12.2012
  • Peer Reviewed
• [Journal Article] Localization of acetylcholine-related molecules in the retina: implication of the communication from photoreceptor to retinal pigment epithelium. (2012)
  • Author(s): Hidetaka Matsumoto
  • Journal Title: PloS one Volume: 7 Issue: 8 Pages: e42841-e42841
  • DOI: 10.1371/journal.pone.0042841
  • Peer Reviewed
• [Journal Article] Synapse type-independent degradation of the endocannabinoid 2-arachidonoylglycerol after retrograde synaptic suppression (2012)
  • Author(s): Tanimura A
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Issue: 30 Pages: 12195
  • DOI: 10.1073/pnas.1204404109
  • Peer Reviewed
• [Journal Article] Lack ofmolecular-anatomical evidence forGABAergic influence on axon initialsegment of cerebellar Purkinje cells by the pinceau formation (2012)
  • Author(s): Iwakura A, Uchigashima M, Miyazaki T,Yamasaki M, Watanabe M
  • Journal Title: J Neurosci Volume: 32(27) Issue: 27 Pages: 9438-48
  • DOI: 10.1523/jneurosci.1651-12.2012
  • Peer Reviewed
• [Journal Article] Striatal direct pathway modulates response time in execution of visual discrimination. (2012)
  • Author(s): Fukabori R
  • Journal Title: Eur. J. Neurosci. Volume: 35巻 Issue: 5 Pages: 784-797
  • DOI: 10.1111/j.1460-9568.2012.08005.x
  • Peer Reviewed
• [Journal Article] TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and indices neuritogenesis (2012)
  • Author(s): Yaguchi, H., Okumura, F., Takahashi, H., Kano, T., Kameda, H., Uchigashima, M., Tanaka, S., Watanabe, M., Sasaki, H. and Hatakeyama, S.
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 15 Pages: 12050-12059
  • DOI: 10.1074/jbc.m111.307678
  • Peer Reviewed
• [Journal Article] Unbalance of CB1 receptors expressed in GABAergic and glutamatergic neurons in a transgenic mouse model of Huntington's disease (2012)
  • Author(s): Chiodi, Valentina ; Uchigashima, Motokazu ; Beggiato, Sarah, et
  • Journal Title: Neurobiology of Disease Volume: 45(3) Issue: 3 Pages: 983-991
  • DOI: 10.1016/j.nbd.2011.12.017
  • Peer Reviewed
• [Journal Article] Selective Neural Pathway Targeting Reveals Key Roles of Thalamostriatal Projection in the Control of Visual Discrimination (2011)
  • Author(s): Kato, S.; Kuramochi, M.; Kobayashi, K., et al
  • Journal Title: Journal of Neuroscience Volume: 31(47) Issue: 47 Pages: 17169-17179
  • DOI: 10.1523/jneurosci.4005-11.2011
  • Peer Reviewed
• [Journal Article] Molecular and morphological configuration for 2-Arachidonoylglycerol-mediated retrograde signaling at mossy cell-granule cell synapses in the dentate gyrus (2011)
  • Author(s): Uchigashima, M.; Yamazaki, M.; Yamasaki, M., et al
  • Journal Title: Journal of Neuroscience Volume: 31 Issue: 21 Pages: 7700
  • DOI: 10.1523/jneurosci.5665-10.2011
  • NAID: 120003521155
  • Peer Reviewed
• [Presentation] 黒質線条体ドーパミンシナプスは GABA 作動性シナプスの分子機構を利用した係留性接着装置である (2013)
  • Author(s): 内ヶ島基政
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポートホール高松・かがわ国際会議場(香川県)
• [Presentation] 黒質線条体ドーパミンシナプスはGABA作動性シナプスの分子機構を利用した係留性接着装置である (2013)
  • Author(s): 内ヶ島基政
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: サンポートホール高松・かがわ国際会議場（香川県）
• [Presentation] ドーパミンは背外側線条体においてシナプス伝達とボリューム伝達の中間的伝達様式を示す (2012)
  • Author(s): 内ヶ島基政
  • Organizer: 第58回日本解剖学会東北・北海道連合支部学術集会
  • Place of Presentation: 山形大学(山形市)
• [Presentation] The mode of nigrostriataldopaminergic transmission isintermediate between wired and volumetransmission (2012)
  • Author(s): 内ヶ島基政
  • Organizer: 第58回日本神経科学大会(NEUROSCIENCE 2012)
  • Place of Presentation: 名古屋国際会議場(愛知県)
• [Presentation] 黒質線条体系ドーパミン投射における伝達様式の再検討 (2012)
  • Author(s): 内ヶ島基政
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学(甲府市)
• [Presentation] ドーパミンは背外側線条体においてシナプス伝達とボリューム伝達の中間的伝達様式を示す (2012)
  • Author(s): 内ヶ島基政
  • Organizer: 第58回日本解剖学会東北・北海道連合支部学術集会
  • Place of Presentation: 山形大学（山形市）
• [Presentation] The mode of nigrostriatal dopaminergic transmission is intermediate between wired and volume transmission. (2012)
  • Author(s): 内ヶ島基政
  • Organizer: 第58回日本神経科学大会（NEUROSCIENCE 2012）
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] 黒質線条体系ドーパミン投射における伝達様式の再検討 (2012)
  • Author(s): 内ヶ島基政
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学(甲府)
• [Presentation] Immunohistochemicalcharacterization of dopaminergicsynapses in the mouse striatum (2011)
  • Author(s): 内ヶ島基政
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] ボリューム伝達様式を介した線条体におけるドーパミン神経伝達 (2011)
  • Author(s): 内ヶ島基政
  • Organizer: 第57回日本解剖学会東北・北海道連合支部学術集会
  • Place of Presentation: 岩手大学(盛岡市)
• [Presentation] Molecular and morphologicalconfiguration for2-arachidonoylglycerol-mediatedretrograde signaling at mossycell-granule cell synapses in thedentate gyrus (2011)
  • Author(s): 内ヶ島基政
  • Organizer: 8th IBRO World Congressof Neuroscience
  • Place of Presentation: フィレンツェ(イタリア)、the Fortezza da Basso
• [Presentation] Molecular and morphological configuration for 2-arachidonoylglycerol-mediated retrograde signaling at mossy cell-granule cell synapses in the dentate gyrus (2011)
  • Author(s): 内ヶ島基政
  • Organizer: 8th IBRO World Congress of Neuroscience
  • Place of Presentation: Congress Centre(イタリア)
• [Presentation] ボリューム伝達様式を介した線条体におけるドーパミン神経伝達 (2011)
  • Author(s): 内ヶ島基政
  • Organizer: 第57回日本解剖学会東北・北海道連合支部学術集会
  • Place of Presentation: 岩手大学(盛岡)
• [Presentation] Immunohistochemical characterization of dopaminergic synapses in the mouse striatum (2011)
  • Author(s): 内ヶ島基政
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川)