Visualization of altered brain metabolism in the ischemic mouse brain by advanced-analytical technology

• Project/Area Number: 23810027
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Keio University
• Principal Investigator: SUGIURA Yuki 慶應義塾大学, 医学部, 特任講師 (30590202)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 質量分析 / 神経科学 / 糖代謝 / 脳梗塞 / 虚血 / 死後分解 / 代謝

Research Abstract

Because abnormal presence/absence of bioactive metabolites can induce many diseases,understanding their local production and diffusion within tissues is important. However, visualization technology for such small molecules was lacked. We established imagi

Research Products

• [Journal Article] Microscopic imaging mass spectrometry assisted by on-tissue chemical derivatization for visualizing multiple amino acids in human colon cancer xenografts (2013)
  • Author(s): Toue S, SugiuraY, Kubo A, Ohmura M, Karakawa S, Mizukoshi T, Yoneda J, MiyanoH, Noguchi Y, Kobayshi T, Kabe Y, and Suematsu M
  • Journal Title: Proteomics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Visualization and quantification of cerebral metabolic fluxes of glucose in the awake mice (2013)
  • Author(s): Sugiura Y, Honda K, Kajimura M and Suematsu M
  • Journal Title: Proteomics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Microscopic imaging mass spectrometry assisted by on-tissue chemical derivatization for visualizing multiple amino acids in human colon cancer xenografts (2013)
  • Author(s): Sakino Toue and Yuki Sugiura
  • Journal Title: Proteomics Volume: in press
  • Peer Reviewed
• [Journal Article] Visualization of acetylcholine distribution in central nervous system tissue sections by tandem imaging mass spectrometry. (2012)
  • Author(s): Sugiura Y, Zaima N, Setou M, Ito S, Yao I.
  • Journal Title: Anal Bioanal Chem. Volume: 403 Issue: 7 Pages: 1851-1861
  • DOI: 10.1007/s00216-012-5988-5
  • Peer Reviewed
• [Journal Article] Spatiotemporal alteration of phospholipids and prostaglandins in a rat model of spinal cord injury (2012)
  • Author(s): Hanada M, Sugiura Y, Shinjo R, Masaki N, Imagama S, Ishiguro N, Matsuyama Y, Setou M
  • Journal Title: Anal Bioanal Chem Volume: 403巻 Issue: 7 Pages: 1873-1884
  • DOI: 10.1007/s00216-012-5900-3
  • Peer Reviewed
• [Journal Article] Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics. (2012)
  • Author(s): Yang HJ, Sugiura Y, Ikegami K, Konishi Y, Setou M.
  • Journal Title: J Biol Chem Volume: 287 Pages: 5290-300
  • Peer Reviewed
• [Journal Article] 質量分析法による代謝物質のイメージングと定量 (2012)
  • Author(s): 杉浦 悠毅
  • Journal Title: 実験医学 Volume: 30 Pages: 1949-1954
• [Journal Article] Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics (2012)
  • Author(s): Yang HJ, Sugiura Y, Ikegami K, Konishi Y, Setou M
  • Journal Title: J.Biol.Chem. Volume: 287 Pages: 5290-300
  • Peer Reviewed
• [Journal Article] Spatiotemporal alteration of phospholipids and prostaglandins in a rat model of spinal cord injury (2012)
  • Author(s): Hanada M, Sugiura Y, Shinjo R, Imagama S, Ishiguro N, Matsuyama Y, Setou M
  • Journal Title: Anal.Bioanal.Chem. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Visualization of acetylcholine distribution in central nervous system tissue sections by tandem imaging mass spectrometry (2012)
  • Author(s): Sugiura Y, Zaima N, Setou M, Ito S, Yao I
  • Journal Title: Anal.Bioanal.Chem. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Neuroaxonal dystrophy in calcium-independent phospholipase A2β deficiency results from insufficient remodeling and degeneration of mitochondrial and presynaptic membranes. (2011)
  • Author(s): Beck G, Sugiura Y, Shinzawa K, Kato S, Setou M, Tsujimoto Y, Sakoda S, Sumi-Akamaru H
  • Journal Title: J Neurosci Volume: 31 Pages: 11411-20
  • Peer Reviewed
• [Journal Article] Hydroxylated and non-hydroxylated sulfatide are distinctly distributed in the human cerebral cortex. (2011)
  • Author(s): Yuki D, Sugiura Y, Zaima N, Akatsu H, Hashizume Y, Yamamoto T, Fujiwara M, Sugiyama K, Setou M.
  • Journal Title: Neuroscience Volume: 193
  • URL: http://doi.org/10.1016/j.neuroscience.2011.07.045
  • Peer Reviewed
• [Journal Article] Hydroxylated and non-hydroxylated sulfatide are distinctly distributed in the human cerebral cortex (2011)
  • Author(s): Yuki D, Sugiura Y, Zaima N, Akatsu H, Hashizume Y, Yamamoto T, Fujiwara M, Sugiyama K, Setou M
  • Journal Title: Neuroscience Volume: 193巻 Pages: 44-53
  • DOI: 10.1016/j.neuroscience.2011.07.045
  • Peer Reviewed
• [Journal Article] Insufficient remodeling of membranes of mitochondria and axon terminals causes neuroaxonal dystrophy in calcium-independent phospholipase A2b deficiency. (2011)
  • Author(s): Beck, G.
  • Journal Title: Journal of Neuroscience Volume: VOL. 31 Issue: 31 Pages: 11411-11420
  • DOI: 10.1523/jneurosci.0345-11.2011
  • Peer Reviewed
• [Presentation] グルコース代謝フラックスのイメージングマススペクトロメトリー (2012)
  • Author(s): 杉浦 悠毅
  • Organizer: 第7回メタボロームシンポジウム
  • Place of Presentation: 山形県鶴岡市
  • Year and Date: 2012-10-10
• [Presentation] グルコース代謝フラックスのイメージングマススペクトロメトリー (2012)
  • Author(s): 杉浦 悠毅
  • Organizer: 第7回メタボロームシンポジウム
  • Place of Presentation: 山形県, 鶴岡市
• [Presentation] 質量分析による生体の代謝可視化 (2011)
  • Author(s): 杉浦 悠毅
  • Organizer: 第6回メタボロームシンポジウム
  • Place of Presentation: 大阪大学
  • Year and Date: 2011-10-14
• [Presentation] 質量分析による生体の代謝可視化-プラクティカルな実験のポイントについて- (2011)
  • Author(s): 杉浦悠毅
  • Organizer: 第6回メタボロームシンポジウム
  • Place of Presentation: 大阪府(大阪大学)(招待講演)
  • Year and Date: 2011-10-14
• [Presentation] イメージングマススペクトロメトリーによる脳の代謝の視覚化 (2011)
  • Author(s): 杉浦 悠毅
  • Organizer: 第36回日本医用マススペクトル学会年会
  • Place of Presentation: ホテル阪急エキスポパーク
  • Year and Date: 2011-09-16
• [Presentation] イメージングマススペクトロメトリーによる脳の代謝の視覚化 (2011)
  • Author(s): 杉浦悠毅
  • Organizer: 第36回日本医用マススペクトル学会年会
  • Place of Presentation: 大阪府(ホテル阪急エキスポパーク)(招待講演)
  • Year and Date: 2011-09-16
• [Presentation] Visualization of cellular energy status and oxidative stress by using multiple metabolite ions in imaging mass spectrometry (2011)
  • Author(s): Sugiura Y, Setou M.
  • Organizer: 59th ASMS Conference On Mass Spectrometry
  • Place of Presentation: Denver, USA
  • Year and Date: 2011-06-04
• [Book] 実験医学別冊: 質量分析実験ガイド (2013)
  • Author(s): 杉浦 悠毅, 末松誠
  • Total Pages: 238
  • Publisher: 羊土社
• [Book] 実験医学別冊 (2013)
  • Author(s): 杉浦 悠毅 (編)
  • Total Pages: 238
  • Publisher: 質量分析実験ガイド