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High throughput single molecule manipulation device for real-time observation of DNA binding proteins

Research Project

Project/Area Number 23810034
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Nanomaterials/Nanobioscience
Research InstitutionNagoya University

Principal Investigator

ARATA Hideyuki  名古屋大学, 理学研究科, 特任講師 (00582446)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsMEMS / DNA / 一分子計測 / ハイスループット
Research Abstract

Future goal of this project was to develop high-throughput single molecule system utilizing technical aspects of both bioMEMS and single molecule manipulation. To achieve this goal, we have developed a silicon-based microdevice consists of two microchannels and multiple nanochannels. We also confirmed that a single DNA molecule can be inserted and manipulated in a nanochannel.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • Research Products

    (23 results)

All 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (17 results)

  • [Journal Article] Rapid and sensitive microRNA detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip2012

    • Author(s)
      新田 英之
    • Journal Title

      PLOS ONE

      Volume: 7 Issue: 11 Pages: e48329-e48329

    • DOI

      10.1371/journal.pone.0048329

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid MicroRNA Detection Using Power-Free Microfluidic Chip: Coaxial Stacking Effect Enhances the Sandwich Hybridization2012

    • Author(s)
      Hideyuki Arata, Hiroshi Komatsu, Aishan Han, Kazuo Hosokawa, and Mizuo Maeda
    • Journal Title

      Analyst

      Volume: Vol. 137 Pages: 3234-3237

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid MicroRNA Detection Using Power-Free Microfluidic Chip: Coaxial Stacking Effect Enhances the Sandwich Hybridization2012

    • Author(s)
      Hideyuki Arata, Hiroshi Komatsu, Aishan Han, Kazuo Hosokawa, Mizuo Maeda
    • Journal Title

      Analyts

      Volume: 137 Issue: 14 Pages: 3234-3237

    • DOI

      10.1039/c2an16154k

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 相同組み換えタンパク質によるDNAねじり運動の実時間計測2011

    • Author(s)
      新田 英之
    • Journal Title

      生物物理

      Volume: 51 Pages: 2-3

    • NAID

      10029139976

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マイクロ高速加熱デバイスを用いた生体分子挙動の顕微鏡下ミリ秒計測2011

    • Author(s)
      新田 英之、藤田博之
    • Journal Title

      分析化学

      Volume: Vol. 60, No. 4 Pages: 325-332

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] マイクロ高速加熱デバイスを用いた生体分子挙動の顕微鏡下ミリ秒計測2011

    • Author(s)
      新田英之、藤田博之
    • Journal Title

      分析化学

      Volume: 60 Pages: 325-332

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 花粉管内標的遺伝子の機能阻害と花粉管誘引アッセイに対する新アプローチ2012

    • Author(s)
      水多陽子, 洞出光洋, 加地範匡, 後藤宏旭, 新田英之, 東山哲也
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Plant-on-a-Chip Microfluidic-System for Quantitative Analysis of Pollen Tube Guidance by Signaling Molecule: Towards Cell-to-Cell Communication Study2012

    • Author(s)
      Mitsuhiro Horade, Yoko Mizuta, Noritada Kaji, Tetsuya Higashiyama, and Hideyuki Arata
    • Organizer
      microTAS
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Sub-Attomole Detection of MicroRNA in Twenty Minutes Using Power-Free Microfluidic Chip: Towards Popint-of-Care Testing2012

    • Author(s)
      Hideyuki Arata, Hiroshi Komatsu, Kazuo Hosokawa, and Mizuo Maeda
    • Organizer
      microTAS
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] PDMSマイクロチャネルを用いた花粉管細胞伸長の計測と制御2012

    • Author(s)
      洞出 光洋, 水多 陽子, 加地 範匡, 新田英之, 東山 哲也
    • Organizer
      第25回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      崇城大学(熊本県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Plant-on-a-Chip Microfluidic-System for Quantitative Analysis of Pollen Tube Guidance by Signaling Molecule: Towards Cell-to-Cell Communication Study2012

    • Author(s)
      Mitsuhiro Horade, Yoko Mizuta, Noritada Kaji, Tetsuya Higashiyama, Hideyuki Arata
    • Organizer
      MicroTAS 2012
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] PDMSマイクロチャネルを用いた花粉管細胞伸長の計測と制御2012

    • Author(s)
      洞出 光洋, 水多 陽子, 加地 範匡, 新田 英之, 東山 哲也
    • Organizer
      第25回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      崇城大学(熊本市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 自律駆動マイクロ流体チップを用いたマイクロRNA高感度検出2011

    • Author(s)
      小松仁, 新田英之, 細川和生, 前田瑞夫
    • Organizer
      第23回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      千葉大学
    • Year and Date
      2011-10-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] HIGHLY SENSITIVE MicroRNA DETECTION USING GOLD-NANO-PARTICLES ON POWER-FREE MICROFLUIDIC CHIP : TOWARDS POINT-OF-CARE EARLY-STAGE CANCER DIAGNOSIS2011

    • Author(s)
      Hideyuki Arata, Hiroshi Komatsu, Aishan Han, Kazuo Hosokawa, Mizuo Maeda
    • Organizer
      MicroTAS 2011
    • Place of Presentation
      米国、シアトル
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微細加工技術を用いて製作した極微小デバイスによるタンパク質分子の顕微鏡下ミリ秒計測2011

    • Author(s)
      新田 英之, 藤田 博之
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2011-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 微細加工技術を用いて製作したごく微小デバイスによるタンパク質分子の顕微鏡下ミリ秒計測2011

    • Author(s)
      新田英之, 藤田博之
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 自律駆動マイクロ流体チップと金ナノ粒子によるマイクロRNAの検出2011

    • Author(s)
      小松 仁, 新田 英之, 韓 愛善, 細川 和生, 前田 瑞生
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2011-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 自律駆動マイクロ流体チップと金ナノ粒子によるマイクロRNAの検出2011

    • Author(s)
      小松仁, 新田英之, 韓愛善, 細川和生, 前田瑞生
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] MicroRNA Detection in Thirty Minutes Using Gold Nanoparticles on Power-Free Microfluidic Chip2011

    • Author(s)
      H. Komatsu, H. Arata, A. Han, K. Hosokawa, M. Maeda
    • Organizer
      JAIMA Discussion on Analytical Science & Technology, JAIMA Conference
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2011-09-08
    • Related Report
      2012 Final Research Report
  • [Presentation] MicroRNA Detection in Thirty Minutes Using Gold Nanoparticles on Power-Free Microfluidic Chip2011

    • Author(s)
      H.Komatsu, H.Arata, A.Han, K.Hosokawa, M.Maeda
    • Organizer
      JAIMA Discussion on Analytical Science & Technology, JAIMA Conference
    • Place of Presentation
      幕張メッセ、千葉県
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Highly Sensitive MicroRNA Detection Using Gold-Nano-Particles on Power-Free Microfluidic Chip: Towards Point-of-Care Early-Stage Cancer Diagnosis2011

    • Author(s)
      Hideyuki Arata, Hiroshi Komatsu, Aishan Han, Kazuo Hosokawa and Mizuo Maeda
    • Organizer
      Proc. of microTAS
    • Place of Presentation
      Seattle
    • Related Report
      2012 Final Research Report
  • [Presentation] 自律駆動マイクロ流体チップを用いたマイクロRNA高感度検出2011

    • Author(s)
      小松 仁, 新田 英之, 細川 和生, 前田瑞夫
    • Organizer
      第23回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      千葉大学(千葉県)
    • Related Report
      2012 Final Research Report
  • [Presentation] 花粉管ガイダンスの分子実体解明に向けてーマイクロ流体デバイスを用いた試み

    • Author(s)
      水多 陽子, 洞出 光洋, 後藤 宏旭, 加地 範匡, 新田 英之, 東山 哲也
    • Organizer
      日本植物形態学会第24回大会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス(兵庫県)
    • Related Report
      2012 Annual Research Report

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Published: 2011-09-05   Modified: 2019-07-29  

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