| Project/Area Number |
23870012
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 根端メリステム / 活性酸素種 / 転写因子細胞周期 / 転写因子 / 細胞周期 / 転写制御 / 細胞機能転換 / 活性酸素 / 発生発達 / 根端成長 |
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| Research Abstract |
To elucidate the mechanism of how ROS regulates the plant root growth, I analyzed that the effect of ROS to the cell cycle progression by using the molecular biology technique. The present study investigated the effect of hydrogen peroxide (H2O2), a ROS compound, on cell cycle-related gene expression. Gene expression analyses coupled with microdissected sections of the developmental zone of Arabidopsis root tips revealed that H2O2 affects the expression of cell cycle-related genes. Additionally, ROS scavenging enzymes were found to play an important role in the root growth phenotype induced by H2O2. Specifically, root growth inhibition by H2O2 was diminished in transgenic Arabidopis overexpressing peroxidase but increased in a catalase2 (cat2) mutant. Overall, these results confirm that ROS function not only as stress-related compounds but that they also function as signaling molecules to regulate the progression of the cell cycle in root tips.
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