Transcriptional network for regulating the root growth via the ROS

• Project/Area Number: 23870012
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: TSUKAGOSHI Hironaka 名古屋大学, 高等研究院(農), 特任講師 (30594056)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 根端メリステム / 活性酸素種 / 転写因子細胞周期 / 転写因子 / 細胞周期 / 転写制御 / 細胞機能転換 / 活性酸素 / 発生発達 / 根端成長

Research Abstract

To elucidate the mechanism of how ROS regulates the plant root growth, I analyzed that the effect of ROS to the cell cycle progression by using the molecular biology technique. The present study investigated the effect of hydrogen peroxide (H2O2), a ROS compound, on cell cycle-related gene expression. Gene expression analyses coupled with microdissected sections of the developmental zone of Arabidopsis root tips revealed that H2O2 affects the expression of cell cycle-related genes. Additionally, ROS scavenging enzymes were found to play an important role in the root growth phenotype induced by H2O2. Specifically, root growth inhibition by H2O2 was diminished in transgenic Arabidopis overexpressing peroxidase but increased in a catalase2 (cat2) mutant. Overall, these results confirm that ROS function not only as stress-related compounds but that they also function as signaling molecules to regulate the progression of the cell cycle in root tips.

Research Products

• [Journal Article] Defective root growth triggered by oxidative stress is controlled through the expression of cell cycle-related genes (2012)
  • Author(s): Tsukagoshi H
  • Journal Title: Plant Sci Volume: 197 Pages: 30-39
  • Peer Reviewed
• [Journal Article] 植物の根のサイズ決定に関わる分子メカニズム. (2012)
  • Author(s): 塚越啓央
  • Journal Title: 化学と生物 Volume: 50 Pages: 255-261
  • NAID: 10030819419
• [Journal Article] Defective root growth triggered by oxidative stress is controlled through the expression of cell cycle-related genes. (2012)
  • Author(s): Hironaka Tsukagoshi
  • Journal Title: Plant science Volume: 197 Pages: 30-39
  • DOI: 10.1016/j.plantsci.2012.08.011
  • Peer Reviewed
• [Journal Article] Cell identity regulators link development and stress responses in the Arabidopsis root (2011)
  • Author(s): Iyer-Pascuzzi AS, Jackson T, Cui H, Petricka JJ, Busch W, Tsukagoshi H, Benfey PN.
  • Journal Title: Dev. Cell Volume: 21 Pages: 770-782
  • Peer Reviewed
• [Journal Article] Cell identity regulators link development and stress responses in the Arabidopsis root (2011)
  • Author(s): Lyer-Pscuzzi, AS., Jackson, T., Cui, H., Petricka, JJ., Busch, W., Tsukagoshi, H., Benfey, PN.
  • Journal Title: Developmental cell Volume: 21 Issue: 4 Pages: 770-782
  • DOI: 10.1016/j.devcel.2011.09.009
  • Peer Reviewed
• [Presentation] Defective root growth caused by oxidative stress is regulated through the expression of cell cycle-related genes (2013)
  • Author(s): Tsukagoshi H.
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山
• [Presentation] UPB1 controls transition from proliferation to differentiation in the root via ROS (2013)
  • Author(s): Tsukagoshi H, Busch W, Benfey P.N.
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] Defective root growth caused by oxidative stress is regulated through the expression of cell cycle-related genes. (2013)
  • Author(s): Hironaka Tsukagoshi
  • Organizer: 第５４回日本植物生理学会
  • Place of Presentation: 岡山
• [Presentation] UPB1 controls transition from proliferation to differentiation in the root via ROS (2012)
  • Author(s): 塚越啓央
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都)
  • Year and Date: 2012-03-16
• [Presentation] Analysis of the ROS signaling that affects the root growth (2012)
  • Author(s): Tsukagoshi H, Busch W, Benfey P.N.
  • Organizer: The 23th International Conference on Arabidopsis Research (ICAR)
  • Place of Presentation: Vienna, Austria
• [Presentation] ANALYSIS OF THE ROS SIGNALING THAT AFFECT THE ROOT GROWTH (2012)
  • Author(s): Hironaka Tsukagoshi
  • Organizer: The 23th International Conference on Arabidopsis Research (ICAR)
  • Place of Presentation: Vienna, Austria
• [Presentation] UPB1, which controls transition from proliferation to differentiation in the root tip (2011)
  • Author(s): 塚越啓央
  • Organizer: Intenational symposium, Strategies of Plants against Global environmental change
  • Place of Presentation: 倉敷市芸文館(岡山)(招待講演)
  • Year and Date: 2011-12-10
• [Presentation] 植物根端から分化への移行を制御する新奇転写因子UP BEAT1 (2011)
  • Author(s): 塚越啓央
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学(東京)(招待講演)
  • Year and Date: 2011-09-17
• [Presentation] 植物根端の細胞分裂から細胞分化への移行を調節する転写因子UP BEAT1の解析 (2011)
  • Author(s): 塚越啓央
  • Organizer: 阿蘇フロンティアサミット
  • Place of Presentation: 阿蘇グランヴィリホテル(熊本)(招待講演)
  • Year and Date: 2011-08-23
• [Presentation] UPB1, which controls transition from proliferation to differentiation in the root tip (2011)
  • Author(s): Tsukagoshi H
  • Organizer: International Symposium, Strategies of Plants against Global Environmental Change
  • Place of Presentation: 倉敷
• [Presentation] 植物根端における細胞分裂から分化への移行を制御する新奇転写因子UP BEAT1 (2011)
  • Author(s): Tsukagoshi H, Busch W, Benfey P.N.
  • Organizer: 日本植物学会第75回大会シンポジウム「根の発生の分子機構」
  • Place of Presentation: 東京
• [Presentation] UPB1,植物根端の細胞分裂から細胞分化への移行を調節する転写因子UP BEAT1の解析 (2011)
  • Author(s): Tsukagoshi H, Busch W, Benfey P.N
  • Organizer: 岡田学術創成研究費 阿蘇フロンティアサミットシンポジウム、植物の形作りの明日を語る
  • Place of Presentation: 阿蘇
• [Remarks]
  • URL: http://hiroarabidoros.the-ninja.jp
• [Remarks] HIRO's web
  • URL: http://hiroarabidoros.the-ninja.jp
• [Remarks]
  • URL: http://hiroarabidoros.the-ninja.jp/