Functional analysis of viral RNA recognition sensor MDA5

• Project/Area Number: 23890004
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Hokkaido University
• Principal Investigator: ADACHI Yoshihirokurisutofua 北海道大学, 遺伝子病制御研究所, 博士研究員 (10616204)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 自然免疫 / MDA5 / SCI2 / RIG-I

Research Abstract

Previous reports shown that RIG-I-like receptor, includes RIG-I, MDA5 senses the presence of virus specific double-strand RNA (dsRNA), thereby activating innate immune system in the cells invaded by virus.Here our study identified that SCI2 bind with Polyinosinic:polycytidylic acid (poly I:C), subsequent serve as positive regulatory molecule in the downstream innate immunity-related inflammatory pathways.Our data, moreover, not only show SCI2 plays a potent role for stimulating IFN activation during viral infection, but bind with MDA5, sensor of poly I:C, suggesting that SCI2 participate in the regulation of MDA5-mediated antiviral innate immunity.