Relationship between lung metastases of osteosarcoma and coagulation-fibrinolytic factors

• Project/Area Number: 23890070
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Kanazawa University
• Principal Investigator: KIMURA Hiroaki 金沢大学, 医学系, 助教 (50608693)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨肉腫 / 肺転移 / プラスミノーゲン活性化因子インヒビタ-1 (PAI-1) / ウロキナーゼ型プラスミノ-ゲン活性化因子 / 蛍光蛋白 / uPA / PAI-1 / 凝固・線溶因子

Research Abstract

With use of human osteosarcoma cell line “143B dual color”, in which GFP is expressing in nucleus and RFP is expressing in cytoplasm, we first developed multiple cell lines that have multiple metastatic potential. In each cell line, we evaluated expression level of uPA, PAI-1, and TF by ELIZA and PCR. The result showed strong relationship between metastatic potential and expression of uPA & PAI-1. We have now performed shRNA of uAP & PAI-1 for 143B cell line. Then we will evaluate how metastatic potential change after suppression of uPA and PAI-1 in osteosarcoma.

Research Products

• [Journal Article] Imaging the efficacy of UVC irradiation on superficial brain tumors and metastasis in live mice at the subcellular level. (2013)
  • Author(s): Momiyama M, Suetsugu A, Kimura H, Kishimoto H, Aki R, Yamada A, Sakurada H, Chishima T, Bouvet M, Endo I, Hoffman RM.
  • Journal Title: J Cell Biochem Volume: 114 (2) Issue: 2 Pages: 428-34
  • DOI: 10.1002/jcb.24381
  • Peer Reviewed
• [Journal Article] Imaging the efficacy of UVC irradiation on superficial brain tumors and metastasis in live mice at the subcellular level (2013)
  • Author(s): Momiyama M
  • Journal Title: J Cell Biochem Volume: 114 Pages: 428-34
  • Peer Reviewed
• [Journal Article] Inhibition and eradication of human glioma with tumor-targeting Salmonella typhimurium in an orthotopic nude-mouse model. (2012)
  • Author(s): Momiyama M, Zhao M, Kimura H, Tran B, Chishima T, Bouvet M, Endo I, Hoffman RM.
  • Journal Title: Cell Cycle Volume: 11(3) Issue: 3 Pages: 628-32
  • DOI: 10.4161/cc.11.3.19116
  • Peer Reviewed
• [Journal Article] Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice (2012)
  • Author(s): Kimura H, Tome Y, Momiyama M, Hayashi K, Tsuchiya H, Bouvet M, Hoffman RM
  • Journal Title: Int J Cancer Volume: 131 Issue: 9 Pages: 2027-33
  • DOI: 10.1002/ijc.27475
  • Peer Reviewed
• [Journal Article] Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice (2012)
  • Author(s): Kimura H,
  • Journal Title: Int J Cancer. Volume: 131 Pages: 2027-33
  • Peer Reviewed
• [Journal Article] Caffeine Induces Apoptosis of Osteosarcoma Cells by Inhibiting AKT/mTOR/S6K, NF-κB and MAPK Pathways. (2012)
  • Author(s): Miwa S
  • Journal Title: Anticancer Res Volume: 32 Pages: 3643-9
  • Peer Reviewed
• [Journal Article] Functional outcomes after total scapulectomy for malignant bone or soft tissue tumors in the shoulder girdle (2011)
  • Author(s): Hayashi K, Karita M, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Tsuchiya H
  • Journal Title: Int J Clin Oncol Volume: 16 Issue: 5 Pages: 568-73
  • DOI: 10.1007/s10147-011-0229-z
  • NAID: 10030246962
  • Peer Reviewed
• [Journal Article] Activity of bone morphogenetic protein-7 after treatment at various temperatures : Freezing vs. pasteurization vs. allograft (2011)
  • Author(s): Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H
  • Journal Title: Cryobiology Volume: 63 Issue: 3 Pages: 235-239
  • DOI: 10.1016/j.cryobiol.2011.09.001
  • NAID: 120003497601
  • Peer Reviewed
• [Presentation] Anti-β1 Integrin Antibody Inhibits Osteosarcoma Lung Seeding and Metastasis (2012)
  • Author(s): Kimura H
  • Organizer: 9thAsian-PacificMusculoSkeletal Tumor Society meeting
  • Place of Presentation: Hilton Kuala Lumpur (Malaysia)
  • Year and Date: 2012-09-12
• [Presentation] FISHを用いた軟部肉腫におけるMDM2遺伝子増幅の解析 (2012)
  • Author(s): 木村浩明
  • Organizer: 第85回日本整形外科学会総会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2012-05-18
• [Presentation] Real time imaging of single sarcoma cell dynmics of lung metastasis (2012)
  • Author(s): Kimura H
  • Organizer: AAOS annual meeting 2012
  • Place of Presentation: Moscone center (USA)
  • Year and Date: 2012-02-07
• [Presentation] Effect of anti-β1 integrin antibody on lung seeding of osteosarcoma cells in live mice visualized by single-cell in vivo imaging (2012)
  • Author(s): Kimura H
  • Organizer: 2012 Annual Meeting of American Society of Clinical Oncology
  • Place of Presentation: McCormic Place,Chicago, IL,USA
• [Presentation] FISHを用いた軟部肉腫におけるMDM2遺伝子増幅の解析 (2012)
  • Author(s): 木村浩明
  • Organizer: 第85回日本整形外科学会総会
  • Place of Presentation: 京都国際会議場、京都市、京都府
• [Presentation] Long-term follow-up of cannulation for solitary bone cysts (2011)
  • Author(s): Kimura H
  • Organizer: 16thGeneral meeting of International Society of Limb-Salvage
  • Place of Presentation: National Convention Center (China)
  • Year and Date: 2011-09-18
• [Presentation] 蛍光蛋白発現がん細胞を用いた肺転移形成のリアルタイムイメージング (2011)
  • Author(s): 木村浩明
  • Organizer: 第44回日本整形外科学会 骨軟部腫瘍学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-07-15