Analysis of biomarkers specific for cancer stem cell in hepatocellular carcinoma usingmass spectrometry
| Project/Area Number |
23890164
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
DOI Koichi 熊本大学, 医学部附属病院, 非常勤診療医師 (40608858)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | Side population 細胞 / ATP-Binding Cassette トランスポーター / Side population細胞 / 肝細胞癌 / 質量分析法 |
|---|
| Research Abstract |
The aim of this study was to identify the biomarker specific for side popupation (SP)cell in hepatocellular carcinoma. SP cells and major population (MP) cells in both welldifferentiated and poorly differentiated cell lines were sorted for protein expressionanalysis. We found 66kDa protein which is specific for SP cells. Mass spectrometricanalysis showed two candidate proteins, Alb and TCP1. However, these proteins were notoverexpressed in SP cells using Western blot analysis, suggesting that proteomic analysisstill needs some improvements in its process.
|
Report
(3 results)
Research Products
(35 results)
-
-
[Journal Article] Assessment of safety in hepaticresection for hepatocellularcarcinoma while focusing on indirecthyperbilirubinemia.2013
Author(s)
Masuda T, Beppu T, Nakagawa S, OkabeH, Chikamoto A, Mima K, Otao R, Hayashi H, Doi K, Ishiko T, Takamori H, Baba H
-
Journal Title
J Hepatobiliary Pancreat Sci
Volume: 20
Issue: 3
Pages: 370-4
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Liver functional volumetry forportal vein embolization using anewly-developed 99mtc-galactosylhuman serum albumin scintigraphyspect-computed tomography fusionsystem.2011
Author(s)
Beppu T, Hayashi H, Okabe H, Masuda T,Mima K, Sugiyama S, Horino K, Otao R,Chikamoto A, Doi K, Ishiko T, TakamoriH, Yoshida M, Shiraishi S, YamashitaY, Baba H
-
Journal Title
J Gastroenterol
Volume: 46
Issue: 7
Pages: 938-43
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
