SUMOylation of small G proteins in malignant melanomas

• Project/Area Number: 23890198
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Iwate Medical University
• Principal Investigator: TSUNODA Kanako 岩手医科大学, 医学部, 助教 (10611030)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 悪性黒色腫 / SUMO / NACC1 / RAC1 / Small G蛋白 / HDAC6 / 皮膚科学 / small G / 浸潤 / 転移 / 翻訳後修飾 / SUMO化

Research Abstract

NACC1 is a member of pluripotent transcription factor, and associates with malignant phenotypes of tumor cells. The present study investigated that the molecular mechanisms of NACC1 in malignant melanomas. We represented that NACC1 directly bound to cortactin, and deacetylated cortactin. This deacetylation introduced acceleration of motility and invasion of tumor cells. The SUMOlylation occurred at NACC1-K167 site, but did not in RAC1. CYLD1 induced-RAC1 activation has been also demonstrated in melanoma cells. These results suggested that NACC1 related proteins may regulated motility and invasion activities of melanoma cells.

Research Products

• [Journal Article] Downregulation of cylindromatosis gene, CYLD, confers a growth advantage on malignant melanoma cells while negatively regulating their migration activity (2012)
  • Author(s): Ishikawa Y, Tsunoda K, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.
  • Journal Title: Int J Oncol Volume: 41(1) Pages: 53-60
  • DOI: 10.3892/ijo.2012.1424
  • Peer Reviewed
• [Journal Article] Downregulation of cylindromatosis gene, CYLD, confers a growth advantage on malignant melanoma cells while negatively regulating their migration activity (2012)
  • Author(s): Ishikawa Y
  • Journal Title: Int J Oncol Volume: (in press) Issue: 3 Pages: 314-5
  • DOI: 10.1111/j.1346-8138.2011.01259.x
  • Peer Reviewed
• [Journal Article] Downregulation of microRNA-211 is involved in expression of preferentially expressed antigen of melanoma in melanoma cells (2011)
  • Author(s): Sakurai E, Maesawa C, Shibazaki M, Yasuhira S, Oikawa H, Sato M, Tsunoda K, Ishikawa Y, Watanabe A, Takahashi K, Akasaka T, Masuda T
  • Journal Title: Int J Oncol Volume: 39 Pages: 665-672
  • DOI: 10.3892/ijo.2011.1084
  • Peer Reviewed
• [Journal Article] Nucleusaccumbens-associated 1 contributes to cortactin deacetylation and augments the migration of melanoma cells. (2011)
  • Author(s): Tsunoda K, Oikawa H, Tada H, Tatemichi Y, Muraoka S, Miura S, Shibazaki M,Maeda F, Takahashi K, Akasaka T, Masuda T, Maesawa C
  • Journal Title: J Invest Dermatol. Volume: 131(8) Issue: 8 Pages: 1710-9
  • DOI: 10.1038/jid.2011.110
  • Peer Reviewed
• [Journal Article] 悪性黒色腫におけるnucleus accumbens associated 1の発現に関する免疫組織学的研究 (2011)
  • Author(s): 角田 加奈子, 及川 浩樹
  • Journal Title: 岩手医学雑誌(0021-3284) Volume: 63巻1号 Pages: 23-32
  • NAID: 110008601619
  • Peer Reviewed
• [Presentation] cuTipTMによる毛細血管拡張病変の治療経験 (2012)
  • Author(s): 角田加奈子
  • Organizer: 日本皮膚科学会総会
  • Place of Presentation: 京都
• [Presentation] NACC1/HDAC6脱アセチル化機構はアクチンおよび微小間依存性の腫瘍細胞の運動能に影響を与える(NACC1/HDAC6 deacetylation system accelerates tumor cell migration via an actin- and microtubule-dependent process)(英語) (2011)
  • Author(s): 前沢 千早, 多田 広志, 角田 加奈子, 柴崎 晶彦, 安平 進士, 及川 弘樹, 管野 公徳, 石川 雄一, 増田 友之
  • Organizer: 第70回日本癌学会総会記事
  • Place of Presentation: 名古屋
• [Remarks]