Analysis of the mechanism of action of antipsychotics by highly sensitive quantitative proteomics
| Project/Area Number |
23890200
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
ARIME Yosefu 獨協医科大学, 医学部, 助教 (80609404)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 統合失調症 / モデルマウス / 行動 / 抗精神病薬 / 受容体占拠率 / 定量プロテオミクス / 行動試験 / 動物モデル / 神経精神薬理 / 行動薬理 |
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| Research Abstract |
The purpose of this study was to set up the experimental system of quantitative proteomics for mouse brain protein and explore the mechanism of action of antipsychotics. We found that enhanced response to an acute phencyclidine (PCP) challenge in mice previously undergoing chronic PCP treatment, called as sensitization,was significantly attenuated by treatment with clinically relevant antipsychotics dose regimen using osmotic minipump. And, we established a method of cell fractionation, peptides specific for each target protein including membrane protein and simultaneous quantification method for their proteins using LC-MS/MS.
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Report
(3 results)
Research Products
(5 results)
