Isolation and culture of c-kit-positive human cardiac stem cells (CSCs): Aiming for the first clinical trial in Japan

• Project/Area Number: 23890211
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Tokai University
• Principal Investigator: HOSODA Toru 東海大学, 創造科学技術研究機構, 准教授 (50608601)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 再生医学 / 体性幹細胞 / 心臓幹細胞 / c-kit

Research Abstract

In order to prepare for a potential domestic clinical trial, adult and pediatric heart tissues resected during cardiac surgeries were obtained. I have succeeded in reproducing the methods to isolate and culture c-kit-positive human cardiac stem cells. Additionally, immunostaining against c-kit was carried out to confirm the specificity of the sorting procedure. Moreover, population doubling time, BrdU positivity, clonogenicity of the stem cells were examined. These data are compared with clinical information to retrieve correlations.

Research Products

• [Journal Article] Dissecting the molecular relationship among various cardiogenic progenitor cells (2013)
  • Author(s): Dey D, Han L, Bauer M, Sanada F, Oikonomopoulos A, Hosoda T, Unno K, De Almeida P, Leri A, Wu JC
  • Journal Title: Circ Res Volume: 112 Issue: 9 Pages: 1253-62
  • DOI: 10.1161/circresaha.112.300779
  • Peer Reviewed
• [Journal Article] 再生医療-細胞移植治療の進歩と展望- (2013)
  • Author(s): 林 恵美子、細田 徹
  • Journal Title: カレントテラピー Volume: 31 Pages: 406-11
• [Journal Article] 心臓幹細胞とエイジング (2013)
  • Author(s): 林 恵美子、田中千陽、細田 徹
  • Journal Title: Heart View Volume: 17 Pages: 379-84
• [Journal Article] 心臓組織幹細胞 (2013)
  • Author(s): 林 恵美子、細田 徹
  • Journal Title: 血管医学 Volume: 14 Pages: 77-84
• [Journal Article] Dissecting the Molecular Relationship Among Various Cardiogenic Progenitor Cells. (2013)
  • Author(s): Devaveena Dey
  • Journal Title: Circulation Research Volume: 未定
  • Peer Reviewed
• [Journal Article] 心臓組織幹細胞. (2013)
  • Author(s): 林 恵美子
  • Journal Title: 血管医学 Volume: 14 Pages: 77-84
• [Journal Article] 心臓幹細胞とエイジング. (2013)
  • Author(s): 林 恵美子
  • Journal Title: Heart View Volume: 17 Pages: 379-384
• [Journal Article] 再生医療―細胞移植治療の進歩と展望―. (2013)
  • Author(s): 林 恵美子
  • Journal Title: カレントテラピー Volume: 31 Pages: 406-411
• [Journal Article] Cardiomyogenesis in the aging and failing human heart (2012)
  • Author(s): Kajstura J, Rota M, Cappetta D, Ogorek B, Arranto C, Bai Y, Ferreira-Martins J, Signore S, Sanada F, Matsuda A, Kostyla J, Caballero MV, Fiorini C, D'Alessandro DA, Michler RE, Del Monte F, Hosoda T, Perrella MA, Leri A, Buchholz BA, Loscalzo J, Anversa
  • Journal Title: Circulation Volume: 126 Issue: 15 Pages: 1869-81
  • DOI: 10.1161/circulationaha.112.118380
  • Peer Reviewed
• [Journal Article] Tracking chromatid segregation to identify human cardiac stem cells that regenerate extensively the infarcted myocardium (2012)
  • Author(s): Kajstura J, Bai Y, Cappetta D, Kim J, Arranto C, Sanada F, D'Amario D, Matsuda A, Bardelli S, Ferreira-Martins J, Hosoda T, Leri A, Rota M, Loscalzo J, Anversa P
  • Journal Title: Circ Res Volume: 111 Issue: 7 Pages: 894-906
  • DOI: 10.1161/circresaha.112.273649
  • Peer Reviewed
• [Journal Article] 心臓幹細胞の臨床への応用 (2012)
  • Author(s): 細田 徹
  • Journal Title: 心臓 Volume: 44 Pages: 1095-8
• [Journal Article] MicroRNAによるc-kit陽性心臓幹細胞の分化制御 (2012)
  • Author(s): 細田 徹
  • Journal Title: 循環器内科 Volume: 71 Pages: 355-9
  • NAID: 40019322910
• [Journal Article] Cardiomyogenesis in the developing heart is regulated by c-kit-positive cardiac stem cells (2012)
  • Author(s): Ferreira-Martins J, Ogorek B, Cappetta D, Matsuda A, Signore S, D'Amario D, Kostyla J, Steadman E, Ide-Iwata N, Sanada F, Iaffaldano G, Ottolenghi S, Hosoda T, Anversa P, Leri A, Kajstura J, Rota M
  • Journal Title: Circ Res Volume: 110 Issue: 5 Pages: 701-15
  • DOI: 10.1161/circresaha.111.259507
  • Peer Reviewed
• [Journal Article] Carbon 14 Birth Dating of Human Cardiomyocytes (2012)
  • Author(s): Kajstura J, Rota M, Hosoda T, Anversa P, Leri A. Response to Bergmann et al
  • Journal Title: Circ Res Volume: 110 Issue: 1
  • DOI: 10.1161/circresaha.111.259721
  • Peer Reviewed
• [Journal Article] C-kit-positive cardiac stem cells and myocardial regeneration (2012)
  • Author(s): Hosoda T
  • Journal Title: Am J Cardiovasc Dis Volume: 2 Pages: 58-67
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257153/
  • Peer Reviewed
• [Journal Article] Editorial: The Heart heals itself (2012)
  • Author(s): Hosoda T
  • Journal Title: Anatom Physiol Volume: 2 Issue: 01
  • DOI: 10.4172/2161-0940.1000e112
  • Peer Reviewed
• [Journal Article] C-kit-positive cardiac stem cells and myocardial regeneration. (2012)
  • Author(s): Toru Hosoda
  • Journal Title: American Journal of Cardiovascular Disease Volume: 2 Pages: 58-67
  • Peer Reviewed
• [Journal Article] MicroRNAによるc-kit陽性心臓幹細胞の分化制御. (2012)
  • Author(s): 細田 徹
  • Journal Title: 循環器内科 Volume: 71 Pages: 355-359
  • NAID: 40019322910
• [Journal Article] 心臓幹細胞の臨床への応用. (2012)
  • Author(s): 細田 徹
  • Journal Title: 心臓 Volume: 44 Pages: 1095-1098
• [Journal Article] C-kit-positive cardiac stem cells and myocardial regeneration (2012)
  • Author(s): Hosoda T.
  • Journal Title: American Journal of Cardiovascular Disease Volume: 2 Pages: 58-67
  • Peer Reviewed
• [Journal Article] Cardiac stem cells in patients with ischaemic cardiomyopathy (SCIPIO): initial results of a randomized phase 1 trial (2011)
  • Author(s): Bolli R, Chugh AR, D'Amario D, Loughran JH, Stoddard MF, Ikram S, Beache GM, Wagner SG, Leri A, Hosoda T, Sanada F, Elmore JB, Goichberg P, Cappetta D, Solankhi NK, Fahsah I, Rokosh DG, Slaughter MS, Kajstura J, Anversa P
  • Journal Title: Lancet Volume: 378 Issue: 9806 Pages: 1847-57
  • DOI: 10.1016/s0140-6736(11)61590-0
  • Peer Reviewed
• [Journal Article] Response to letter regarding article, "Human cardiac stem cell differentiation is regulated by a mircrine mechanism" (2011)
  • Author(s): Hosoda T, Zheng H, Cabral-da-Silva MC,Sanada F, Ide-Iwata N, Ogorek B, Ferreira-Martins J, Arranto C, D'Amario D, Del Monte F, Urbanek K, D'Alessandro DA, Michler RE, Anversa P, Rota M, Kajstura J, Leri A
  • Journal Title: Circulation Volume: 124 Issue: 17
  • DOI: 10.1161/circulationaha.111.051110
  • Peer Reviewed
• [Journal Article] Identification of a coronary stem cell in the human heart (2011)
  • Author(s): Leri A, Hosoda T, Kajstura J, Anversa P, Rota M
  • Journal Title: J Mol Med Volume: 89 Issue: 10 Pages: 947-59
  • DOI: 10.1007/s00109-011-0769-8
  • Peer Reviewed
• [Journal Article] 心臓幹細胞による劇的な再生医療～ボストンから世界初の報告、神奈川から全国への発信 (2011)
  • Author(s): 細田 徹
  • Journal Title: MRIC Pages: 321-321
  • URL: http://medg.jp/mt/2011/11/vol321.html
• [Journal Article] Effects of age and heart failure on human cardiac stem cell function (2011)
  • Author(s): Cesselli D, Beltrami AP, D'Aurizio F, Marcon P, Bergamin N, Toffoletto B, Pandolfi M, Puppato E, Marino L, Signore S, Livi U, Verardo R, Piazza S, Marchionni L, Fiorini C, Schneider C, Hosoda T, Rota M, Kajstura J, Anversa P, Beltrami CA, Leri A
  • Journal Title: Am J Pathol Volume: 179 Issue: 1 Pages: 349-66
  • DOI: 10.1016/j.ajpath.2011.03.036
  • Peer Reviewed
• [Journal Article] Role of stem cells in cardiovascular biology (2011)
  • Author(s): Hosoda T, Rota M, Kajstura J, Leri A, Anversa P
  • Journal Title: J Thromb Haemost Volume: 9 Suppl 1 Pages: 151-61
  • DOI: 10.1111/j.1538-7836.2011.04363.x
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor-1 receptor identifies a pool of human cardiac stem cells with superior therapeutic potential for myocardial regeneration. (2011)
  • Author(s): D'Amario D, Cabral-Da-Silva MC, Zheng H, Fiorini C, Goichberg P, Steadman E, Ferreira-Martins J, Sanada F, Piccoli M, Cappetta D, D'Alessandro DA, Michler RE, Hosoda T, Anastasia L, Rota M, Leri A, Anversa P, Kajstura J
  • Journal Title: Circ Res Volume: 108 Issue: 12 Pages: 1467-81
  • DOI: 10.1161/circresaha.111.240648
  • Peer Reviewed
• [Journal Article] Functionally competent cardiac stem cells can be isolated from endomyocardial biopsies of patients with advanced cardiomyopathies (2011)
  • Author(s): D'Amario D, et al
  • Journal Title: Circulation Research Volume: 108 Issue: 7 Pages: 857-861
  • DOI: 10.1161/circresaha.111.241380
  • Peer Reviewed
• [Journal Article] The ephrin A1-EphA2 system promotes cardiac stem cell migration after infarction (2011)
  • Author(s): Goichberg P, et al
  • Journal Title: Circulation Research Volume: 108 Issue: 9 Pages: 1071-1083
  • DOI: 10.1161/circresaha.110.239459
  • Peer Reviewed
• [Journal Article] Evidence for human lung stem cells (2011)
  • Author(s): Kaistura J, et al
  • Journal Title: New England Journal of Medicine Volume: 364 Issue: 19 Pages: 1795-1806
  • DOI: 10.1056/nejmoa1101324
  • Peer Reviewed
• [Presentation] The Third-Generation Cardiac Stem Cells Defined by Asymmetric Chromatid Segregation and "Mircrine" Mechanism Contributing to Myocardial Regeneration (2013)
  • Author(s): Hosoda T
  • Organizer: 第77回日本循環器学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2013-03-15
• [Presentation] 心臓幹細胞が創出する医療イノベーション (2013)
  • Author(s): 細田 徹
  • Organizer: 医療分野におけるイノベーションの社会・経済評価研究会
  • Place of Presentation: 東京大学・本郷
  • Year and Date: 2013-02-21
• [Presentation] 心臓幹細胞が形作る未来 (2013)
  • Author(s): 細田 徹
  • Organizer: 日本医師会生涯教育講座
  • Place of Presentation: 旭市ホテルサンモール(千葉)
  • Year and Date: 2013-01-25
• [Presentation] Clinical Applications of Cardiac Stem Cells (2012)
  • Author(s): Hosoda T
  • Organizer: 第3回東海大学テニュアトラック制度シンポジウム
  • Place of Presentation: 東海大学高輪校舎(東京)
  • Year and Date: 2012-12-14
• [Presentation] トピックス/心臓幹細胞による心不全治療 (2012)
  • Author(s): 細田 徹
  • Organizer: 第16回核医学フォーラム
  • Place of Presentation: 東京コンファレンスセンター・品川
  • Year and Date: 2012-11-10
• [Presentation] 心臓幹細胞による心不全治療 (2012)
  • Author(s): 細田 徹
  • Organizer: 第13回Vascular and Brain Conferenc
  • Place of Presentation: 大阪帝国ホテル
  • Year and Date: 2012-11-10
• [Presentation] 心臓幹細胞が形作る未来 (2012)
  • Author(s): 細田 徹
  • Organizer: 第8回総合医学研究所研修会
  • Place of Presentation: 湯河原(神奈川)
  • Year and Date: 2012-10-12
• [Presentation] 心臓幹細胞による心不全治療 (2012)
  • Author(s): 細田 徹
  • Organizer: 第51回幹細胞治療研究フォーラム
  • Place of Presentation: 東京大学医科学研究所(東京)
  • Year and Date: 2012-09-20
• [Presentation] 心臓幹細胞が形作る未来 (2012)
  • Author(s): 細田 徹
  • Organizer: 第45回M&Bホスピタル・ソリューション会
  • Place of Presentation: NTT西日本本社(大阪)
  • Year and Date: 2012-07-18
• [Presentation] 心血管再生医療の臨床応用 (2012)
  • Author(s): 細田 徹
  • Organizer: 新宿Cardiovascular Seminar
  • Place of Presentation: 榊原記念病院(東京)
  • Year and Date: 2012-06-29
• [Presentation] Say hello to 心臓幹細胞 (2012)
  • Author(s): 細田 徹
  • Organizer: ボストン日本人研究者交流会
  • Place of Presentation: マサチューセッツ工科大学(米国)
  • Year and Date: 2012-04-21
• [Presentation] "Second-Generation" Cardiac Stem Cell Therapy for the Failing Heart (2012)
  • Author(s): Hosoda T
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-03-16
• [Presentation] "Second-Generation" Cardiac Stem Cell Therapy for the Failing Heart (2012)
  • Author(s): 細田徹
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡国際会議場他
  • Year and Date: 2012-03-16
• [Presentation] 心臓幹細胞による心不全治療 (2012)
  • Author(s): 細田 徹
  • Organizer: 循環器エキスパートミーティング
  • Place of Presentation: 慶應義塾大学・信濃町
  • Year and Date: 2012-01-24
• [Presentation] 心臓幹細胞による心不全治療 (2012)
  • Author(s): 細田徹
  • Organizer: 循環器エキスパートミーティング
  • Place of Presentation: 慶應義塾大学医学部、東京(招待講演)
  • Year and Date: 2012-01-24
• [Presentation] Pursuit of the "Second-Generation" Cardiac Stem Cell Therapy (2011)
  • Author(s): Hosoda T
  • Organizer: 第2回東海大学テニュアトラック制度シンポジウム
  • Place of Presentation: 東海大学校友会館(東京)
  • Year and Date: 2011-12-19
• [Presentation] Myocardial regeneration utilizing "superior" cardiac stem cells (2011)
  • Author(s): Hosoda T
  • Organizer: 第28回国際心臓研究会 日本部会学術集会
  • Place of Presentation: 学術総合センター(東京)
  • Year and Date: 2011-12-03
• [Presentation] Myocardial regeneration utilizing "superior" cardiac stem cells (2011)
  • Author(s): 細田徹
  • Organizer: 第28回国際心臓研究会日本部会学術集会
  • Place of Presentation: 学術総合センター、東京(招待講演)
  • Year and Date: 2011-12-03
• [Presentation] 心臓幹細胞の臨床応用 (2011)
  • Author(s): 細田徹
  • Organizer: 虚血性心疾患セミナー
  • Place of Presentation: ラジオ日経(オンエア)(招待講演)
  • Year and Date: 2011-11-08
• [Presentation] 心臓幹細胞と再生 (2011)
  • Author(s): 細田 徹
  • Organizer: 第4回Translational Research Conference
  • Place of Presentation: 日本大学医学部・板橋
  • Year and Date: 2011-08-09
• [Presentation] 心臓幹細胞と再生 (2011)
  • Author(s): 細田徹
  • Organizer: 第4回Translational Research Conference
  • Place of Presentation: 日本大学医学部、東京(招待講演)
  • Year and Date: 2011-08-09
• [Presentation] Myocyte Turnover in the Adult Human Heart (2011)
  • Author(s): Hosoda T
  • Organizer: 第75回日本循環器学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-08-03
• [Presentation] Myocyte Turnover in the Adult Human Heart (2011)
  • Author(s): 細田徹
  • Organizer: 第75回日本循環器学会学術集会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2011-08-03
• [Presentation] wo Distinct Subpopulations of Adult Human Cardiac Stem Cells Contribute to the Therapeutic Regeneration of the Failing Heart (2011)
  • Author(s): Hosoda T
  • Organizer: 第23回国際血栓止血学会学術集会
  • Place of Presentation: 国際会議場(京都)
  • Year and Date: 2011-07-26
• [Presentation] Two Distinct Subpopulations of Adult Human Cardiac Stem Cells Contribute to the Therapeutic Regeneration of the Failing Heart (2011)
  • Author(s): 細田徹
  • Organizer: 第23回国際血栓止血学会学術集会
  • Place of Presentation: 国際会議場、京都
  • Year and Date: 2011-07-26
• [Presentation] Say hello to 心臓幹細胞.
  • Author(s): 細田 徹
  • Organizer: ボストン日本人研究者交流会
  • Place of Presentation: マサチューセッツ州、米国
  • Invited
• [Presentation] 心血管再生医療の臨床応用.
  • Author(s): 細田 徹
  • Organizer: 新宿Cardiovascular Seminar
  • Place of Presentation: 榊原記念病院、東京
  • Invited
• [Presentation] 心臓幹細胞が形作る未来.
  • Author(s): 細田 徹
  • Organizer: Ｍ＆Ｂホスピタル・ソリューション会
  • Place of Presentation: NTT西日本本社、大阪
  • Invited
• [Presentation] 心臓幹細胞による心不全治療.
  • Author(s): 細田 徹
  • Organizer: 第51回 幹細胞治療研究フォーラム
  • Place of Presentation: 東京大学医科学研究所
  • Invited
• [Presentation] 心臓幹細胞が形作る未来.
  • Author(s): 細田 徹
  • Organizer: 第8回 総合医学研究所 研修会
  • Place of Presentation: 湯河原、神奈川
• [Presentation] 心臓幹細胞による心不全治療.
  • Author(s): 細田 徹
  • Organizer: 第13回 Vascular and Brain Conference
  • Place of Presentation: 大阪帝国ホテル
  • Invited
• [Presentation] トピックス／心臓幹細胞による心不全治療.
  • Author(s): 細田 徹
  • Organizer: 第16回 核医学フォーラム
  • Place of Presentation: 東京コンファレンスセンター・品川
  • Invited
• [Presentation] Clinical Applications of Cardiac Stem Cells.
  • Author(s): Toru Hosoda
  • Organizer: 第3回 東海大学テニュアトラック制度シンポジウム
  • Place of Presentation: 東海大学高輪校舎
• [Presentation] 心臓幹細胞が形作る未来.
  • Author(s): 細田 徹
  • Organizer: 日本医師会生涯教育講座
  • Place of Presentation: 匝瑳地区、千葉
  • Invited
• [Presentation] 心臓幹細胞が創出する医療イノベーション.
  • Author(s): 細田 徹
  • Organizer: 医療分野におけるイノベーションの社会・経済評価研究会
  • Place of Presentation: 文京区、東京
  • Invited
• [Presentation] The Third-Generation Cardiac Stem Cells Defined by Asymmetric Chromatid Segregation and “Mircrine” Mechanism Contributing to Myocardial Regeneration.
  • Author(s): Toru Hosoda
  • Organizer: 第77回 日本循環器学会学術集会
  • Place of Presentation: パシフィコ横浜、神奈川
• [Presentation] 心臓幹細胞による心不全治療.
  • Author(s): 細田 徹
  • Organizer: 第53回 城西循環器病研究会
  • Place of Presentation: 新宿
  • Invited
• [Book] 臨床試験事例. （再生医療事業の課題解決のための手引書） (2013)
  • Author(s): 細田 徹
  • Publisher: 技術情報協会
• [Remarks]
  • URL: http://www.u-tokai.ac.jp/tuiist/tt/announcement_hosoda.html
• [Remarks] 東海大学創造科学技術研究機構 研究者紹介 告知・成果・実績
  • URL: http://www.u-tokai.ac.jp/tuiist/tt/announcement_hosoda.html
• [Remarks]
  • URL: http://www.u-tokai.ac.jp/tuiist/tt/announcement_hosoda.html