Development of novel cell detection system targeting microRNAexpression

• Project/Area Number: 23890226
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Fujita Health University
• Principal Investigator: YAMADA Hiroya 藤田保健衛生大学, 医学部, 助教 (80610352)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: フローサイトメトリ / 細胞検出法 / microRNA / 遺伝子検査 / mRNA quantification after FACS / FACS-mQ / 臨床検査 / FACS / 細胞分離 / フローサイトメトリー / In situ hybridization

Research Abstract

Recently, we have established an in-tube in situ hybridization method named mRNA quantification after fluorescence activated cell sorting (FACS-mQ), in which a specific mRNA in a particular cell type is stained with a florescent dye, allowing the stained cells to be selected by FACS without suffering excessive RNA degradation. In this study, we tried to apply this method to micro RNA detecting in the various cells.

Research Products

• [Journal Article] 新規臨床検査法:FACS-mQの開発[Development of mRNA quantification after fluorescence activated cell sorting] (2012)
  • Author(s): 山田宏哉
  • Journal Title: 臨床病理 Volume: 60(2) Pages: 139-144
  • Peer Reviewed
• [Journal Article] Development of mRNA quantification after fluorescence activated cell sorting (2012)
  • Author(s): 山田宏哉
  • Journal Title: 臨床病理 Volume: 60 (2) Pages: 139-144
  • NAID: 10030463158
  • Peer Reviewed
• [Presentation] 住民健診受診者を対象とした脂肪肝における血清microRNA(miR-122,miR-34a)の解析 (2012)
  • Author(s): 山田宏哉、鈴木康司、市野直浩、安藤嘉崇、沢田章、大橋鉱二、寺平良治、井上孝、橋本修二
  • Organizer: 第59回日本臨床検査医学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 2012-11-30
• [Presentation] 住民健診受診者における脂肪肝と血清microRNA(miR-21,miR-34a,miR-122)との関連 (2012)
  • Author(s): 山田宏哉、鈴木康二、井上孝、伊藤宜則、浜島信之、橋本修二
  • Organizer: 第71回日本公衆衛生学会総会
  • Place of Presentation: 山口
  • Year and Date: 2012-10-25
• [Presentation] 住民健診受診者における脂肪肝と血清microRNA(miR-21, miR-34a, miR-122)との関連 (2012)
  • Author(s): 山田宏哉
  • Organizer: 第71回日本公衆衛生学会総会
  • Place of Presentation: 山口
• [Presentation] 住民健診受診者を対象とした脂肪肝における血清microRNA(miR-122, miR-34a)の解析 (2012)
  • Author(s): 山田宏哉
  • Organizer: 第59回日本臨床検査医学会学術総会
  • Place of Presentation: 京都
• [Presentation] シンポジウム次世代臨床検査診断法の最前線新規臨床検査法:FACSmQの開発臨床検査医学会近畿支部総会 (2011)
  • Author(s): 山田宏哉
  • Organizer: 第54回日本検査医学会近畿支部総会
  • Place of Presentation: 滋賀
  • Year and Date: 2011-10-29
• [Presentation] 新規臨床検査法:FACS-mQの開発 (2011)
  • Author(s): 山田宏哉
  • Organizer: 第54回日本検査医学会近畿支部総会
  • Place of Presentation: 滋賀(招待講演)
  • Year and Date: 2011-10-29