Next generation molecular ecology of contrasting adaptive strategies in flowering plants

• Project/Area Number: 24247005
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: Kyushu University
• Principal Investigator: YAHARA Tetsukazu 九州大学, 理学(系)研究科(研究院), 教授 (90158048)
• Co-Investigator(Kenkyū-buntansha): SUYAMA Yoshihisa 東北大学, 農学研究科, 准教授 (60282315) ; TACHIDA Hidenori 九州大学, 大学院理学研究院, 教授 (70216985) ; TESHIMA Kosuke 九州大学, 大学院理学研究院, 助教 (20447593) ; MIMURA Makiko 玉川大学, 農学部, 准教授 (60451689)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥46,280,000 (Direct Cost: ¥35,600,000、Indirect Cost: ¥10,680,000); Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2013: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2012: ¥21,710,000 (Direct Cost: ¥16,700,000、Indirect Cost: ¥5,010,000)
• Keywords: 分子生態学 / 次世代シークエンサー / ゲノム / 花色 / 葉の寿命 / Hemerocallis / Rubus / Taraxacum / 繁殖戦略 / 種分化 / アントシアニン / 進化 / 交雑 / 外来種 / 無性生殖 / 耐病性遺伝子

Outline of Final Research Achievements

Using Next Generation Sequencer, we analyzed genetic backgrounds of evolutionary changes between two pairs of sister species with contrasting adaptive strategies: Hemerocallis fulva and H. citrina having swallowtail-pollinated red flowers and hawkmoth-pollinated yellow flowers, and Rubus palmaus and R. grayanus having deciduous and evergreen leaves. In the Hemerocallis pair, we identified a group of genes of Anthocyanin color-pigments biosynthesis in which expression levels are correlated with the amounts of Anthocyanin color-pigments. This finding supported that changes in a regulatory system of Anthocyanin biosynthesis caused the evolution of flower color. In the Rubus pair, we found that a gene suppressing the receptor of red light (phytochrome) is highly differentiated between species. This finding suggests that divergence of evergreen/deciduous leaves were driven by regulatory changes of a light receptor.

Research Products

• [Journal Article] Difference in flowering time can initiate speciation of nocturnally flowering species. (2015)
  • Author(s): Matsumoto, T., Yasumoto, A. A., Nitta, K., Hirota, K. S., Yahara, T., Tachida, H.
  • Journal Title: Journal of Theoretical Biology Volume: 370 Pages: 61-71
  • DOI: 10.1016/j.jtbi.2015.01.036
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A genome-wide AFLP replacement in a hybrid population derived from two closely related Viola species from contrasting habitats. (2014)
  • Author(s): Toyama H, Kamiyama T, Yahara T.
  • Journal Title: Plant Systematics & Evolution Volume: 301 Issue: 4 Pages: 1073-1084
  • DOI: 10.1007/s00606-014-1137-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Demographic history of a common pioneer tree, Zanthoxylum ailanthoides, reconstructed using isolation-with-migration model. (2014)
  • Author(s): Yoshida T, Tamekuni M, Yahara T, Inomata, Tachida H.
  • Journal Title: Tree Genetics & Genomes Volume: 5 Issue: 5 Pages: 1213-1222
  • DOI: 10.1007/s11295-014-0755-8
  • Peer Reviewed
• [Journal Article] Formation of a hybrid triploid agamosperm on a sexual diploid plant : evidence from progeny tests in Taraxacum platycarpum Dahlst (2014)
  • Author(s): Chika Mitsuyuki・Akihiko Hoya・Hiroyuki Shibaike・Mikio Watanabe・Tetsukazu Yahara
  • Journal Title: Plant Systematics and Evolution Volume: 300 Issue: 5 Pages: 863-870
  • DOI: 10.1007/s00606-013-0925-4
  • Peer Reviewed
• [Journal Article] Pollinator-Mediated Selection on Flower Color, Flower Scent and Flower Morphology of Hemerocallis: Evidence from Genotyping Individual Pollen Grains On the Stigma (2013)
  • Author(s): Hirota SK, Nitta K, Suyama Y, Kawakubo N, Yasumoto AA, Yahara T.
  • Journal Title: PLoS ONE Volume: 8 Issue: 12 Pages: e85601-e85601
  • DOI: 10.1371/journal.pone.0085601
  • Peer Reviewed
• [Journal Article] Directional selection for early flowering is imposed by a re-associated herbivore - but no evidence of directional evolution (2013)
  • Author(s): Fukano Y, Tanaka K, Yahara T.
  • Journal Title: Basic and Applied Ecology Volume: 14 Issue: 5 Pages: 387-395
  • DOI: 10.1016/j.baae.2013.05.009
  • Peer Reviewed
• [Journal Article] Difference in flowering time as an isolating barrier. (2013)
  • Author(s): Matsumoto T, Yasumoto AA, Nitta K, Yahara T and Tachida H
  • Journal Title: Journal of Theoretical Biology Volume: 317 Pages: 161-167
  • DOI: 10.1016/j.jtbi.2012.10.001
  • Peer Reviewed
• [Journal Article] Changes in defense of an alien plant Ambrosia artemislifolia before and after the invasion of a native specialist enemy Ophraella co〓 (2012)
  • Author(s): Yuya Fukano, Tetsukazu Yahara
  • Journal Title: PLoS ONE Volume: 11 Issue: 11 Pages: e49114-e49114
  • DOI: 10.1371/journal.pone.0049114
  • Peer Reviewed
• [Journal Article] Relative role of flower color and scent on pollinator attraction : experimental tests using F1 and F2 hybrids of davlity and nightlily (2012)
  • Author(s): Shun K. Hirota, et al.
  • Journal Title: PLoS ONE Volume: 7 Issue: 6 Pages: e39010-e39010
  • DOI: 10.1371/journal.pone.0039010
  • Peer Reviewed
• [Presentation] 薄暮性および夜行性スズメガによる花形質への選好性 (2015)
  • Author(s): 廣田峻・矢原徹一
  • Organizer: 第62回日本生態学会大会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2015-03-21
• [Presentation] 受粉花粉の直接遺伝解析に基づくアゲハチョウを介した送粉パターンの解明
  • Author(s): 廣田峻，新田梢，陶山佳久，安元暁子，矢原徹一
  • Organizer: 日本生態学会
  • Place of Presentation: 広島
• [Presentation] 気候変動による種の接触と浸透交雑
  • Author(s): 三村真紀子，矢原徹一
  • Organizer: 日本生態学会
  • Place of Presentation: 広島
• [Presentation] タンポポ耐病性遺伝子(R-gene)の種間、集団間変異
  • Author(s): 満行 知花, 矢原 徹一
  • Organizer: 日本生態学会
  • Place of Presentation: 広島