| Project/Area Number |
24247037
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Yamazaki Yuji 大阪大学, 生命機能研究科, 助教 (80527664)
TAMURA Atsushi 大阪大学, 医学系研究科, 助教 (00362525)
Takeuchi Kosei 新潟, 医歯学系, 准教授 (90206946)
Mineta Katsuhiko 北海道大学, 情報科学研究科, 准教授 (40374615)
Ito Masahiko 獨協医科大学, 医学部, 准教授 (70270486)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2014: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2013: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2012: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
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| Keywords | 上皮細胞シート / 細胞間接着装置 / アピカル膜 / イオン・水フロー / クローディン / タイトジャンクション / 水フロー / 細胞骨格 / アピカル膜複合体 / バリアー / シグナル伝達 / 細胞・組織 / 生理学 / 生体分子 |
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| Outline of Final Research Achievements |
The aim of this study was to learn how reciprocal regulation between the cell-cell adhesion of tight junctions (TJs) and the apical membrane defines a unified epithelial system via the cytoskeleton and cell signaling in epithelial cell sheets. Claudins, the primary constituents of TJs, have critical roles as a structural component of the paracellular barrier. A recent crystallographic structural analysis revealed the molecular structure of claudin. Our recent knockout mouse analyses elucidated the physiological relevance of the paracellular barriers/permeability in many biological systems. On the other hand, we recently discovered that a network of microtubules existed just below the apical membrane of the epithelial cell sheet. Since this apical microtubule network appeared to be connected to TJs, we propose that the TJ-apical complex is critical for organizing the TJ and apical membrane systems, and thus for creating and regulating the epithelial barrier and biological systems.
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