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2015 Fiscal Year Final Research Report

How reciprocal regulation of the cell-cell adhesion and the apical membrane defines a unified epithelial system via the cytoskeleton and cell signaling in epithelial cell sheets

Research Project

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Project/Area Number 24247037
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionOsaka University

Principal Investigator

Tsukita Sachiko  大阪大学, 生命機能研究科, 教授 (00188517)

Co-Investigator(Renkei-kenkyūsha) Yamazaki Yuji  大阪大学, 生命機能研究科, 助教 (80527664)
TAMURA Atsushi  大阪大学, 医学系研究科, 助教 (00362525)
Takeuchi Kosei  新潟, 医歯学系, 准教授 (90206946)
Mineta Katsuhiko  北海道大学, 情報科学研究科, 准教授 (40374615)
Ito Masahiko  獨協医科大学, 医学部, 准教授 (70270486)
Project Period (FY) 2012-04-01 – 2016-03-31
Keywords上皮細胞シート / 細胞間接着装置 / アピカル膜 / イオン・水フロー / クローディン / タイトジャンクション
Outline of Final Research Achievements

The aim of this study was to learn how reciprocal regulation between the cell-cell adhesion of tight junctions (TJs) and the apical membrane defines a unified epithelial system via the cytoskeleton and cell signaling in epithelial cell sheets. Claudins, the primary constituents of TJs, have critical roles as a structural component of the paracellular barrier. A recent crystallographic structural analysis revealed the molecular structure of claudin. Our recent knockout mouse analyses elucidated the physiological relevance of the paracellular barriers/permeability in many biological systems. On the other hand, we recently discovered that a network of microtubules existed just below the apical membrane of the epithelial cell sheet. Since this apical microtubule network appeared to be connected to TJs, we propose that the TJ-apical complex is critical for organizing the TJ and apical membrane systems, and thus for creating and regulating the epithelial barrier and biological systems.

Free Research Field

細胞生物学

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Published: 2017-05-10   Modified: 2017-05-22  

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