Molecular analyses of RNA silencing amplification system in plants
| Project/Area Number |
24248010
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
Tesuo Meshi 国立研究開発法人農業生物資源研究所, その他部局等, その他 (40157813)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Masayuki 農業生物資源研究所, 植物科学研究領域・植物-微生物間相互作用研究ユニット, ユニット長 (70192482)
YOSHIKAWA Manabu 農業生物資源研究所, 植物科学研究領域・植物-微生物間相互作用研究ユニット, 主任研究員 (80391564)
ISHIBASHI Kazuhiro 農業生物資源研究所, 植物科学研究領域・植物-微生物間相互作用研究ユニット, 主任研究員 (20611742)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥43,940,000 (Direct Cost: ¥33,800,000、Indirect Cost: ¥10,140,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2014: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Keywords | RISC複合体 / trans-acting siRNA / microRNA / リボソーム / ウイルス / サプレッサー / RISC / RNAサイレンシングサプレッサー / AGO1 / SGS3 |
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| Outline of Final Research Achievements |
Plants have an RNA silencing amplification system, in which secondary small interfering RNAs (siRNAs) are produced from RISC-cleaved RNAs. This system plays a crucial role in virus resistance. To elucidate the molecular mechanisms of RNA silencing amplification, we analyzed Arabidopsis microRNA173-triggered trans-acting siRNA production using an in vitro RISC formation system. We discovered that SGS3 forms a complex that contains AGO1 and cleaved RNAs, and stabilizes RISC-cleaved RNAs. We also found that ribosomes associate with TAS2 RNA that had been thought to be non-coding. Further, we showed that a short open reading frame (ORF) that encompasses the microRNA173 target site on TAS2 RNA positively regulates production of tasiRNAs.
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Report
(5 results)
Research Products
(8 results)
