| Budget Amount *help |
¥43,940,000 (Direct Cost: ¥33,800,000、Indirect Cost: ¥10,140,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2014: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Outline of Final Research Achievements |
Plants have an RNA silencing amplification system, in which secondary small interfering RNAs (siRNAs) are produced from RISC-cleaved RNAs. This system plays a crucial role in virus resistance. To elucidate the molecular mechanisms of RNA silencing amplification, we analyzed Arabidopsis microRNA173-triggered trans-acting siRNA production using an in vitro RISC formation system. We discovered that SGS3 forms a complex that contains AGO1 and cleaved RNAs, and stabilizes RISC-cleaved RNAs. We also found that ribosomes associate with TAS2 RNA that had been thought to be non-coding. Further, we showed that a short open reading frame (ORF) that encompasses the microRNA173 target site on TAS2 RNA positively regulates production of tasiRNAs.
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