| Project/Area Number |
24248030
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Kyushu University |
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Principal Investigator |
KONDO Ryuichiro 九州大学, (連合)農学研究科(研究院), 特任教授 (80091370)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Hirofumi 静岡大学, 農学部, 教授 (70322138)
KAMEI Ichiro 宮崎大学, 農学部, 教授 (90526526)
山岸 賢治 東北農業研究センター環境保全型農業研究領域, その他部局等, 主任研究員 (80355304)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2013: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2012: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
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| Keywords | バイオエタノール / 白色腐朽菌 / 褐色腐朽菌 / リグニン / 微生物分解 / 広葉樹 / 針葉樹 / アルコール発酵 / 針葉樹材 / 広葉樹材 / IFFP / キチリメンタケ / 担子菌 / 糖化酵素 / 脱リグニン / 糖化発酵 / 木質バイオリファイナリー / 木質バイオマス |
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| Outline of Final Research Achievements |
Future expansion has to be based on bioethanol from lignocellulosic materials, such as agricultural residues and forest residues, as well as from dedicated crops.
The white-rot fungus Phlebia sp. strain MG-60 was proposed as a candidate for integrated fungal fermentation process (IFFP), which unifies aerobic delignification and semi-aerobic consolidated biological processing by a single microorganism based on its ability to efficiently degrade lignin and ferment the sugars from cellulose. To improve IFFP, the development of a molecular breeding method for strain MG-60 is necessary. The study is to establish the transformation method for the strain MG-60 and to obtain the over-expressing transformants of lignin-degrading enzyme, manganese peroxidase.The MG-60 transformation provides a useful methodology for widely accessible to interested researches. These results indicate the possibility of metabolic engineering of strain MG-60 for improving IFFP.
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