Studies on the role of LIN28A in mouse trophoblast stem cell

Research Project

Project/Area Number	24248048
Research Category	Grant-in-Aid for Scientific Research (A)
Allocation Type	Single-year Grants
Section	一般
Research Field	Applied animal science
Research Institution	The University of Tokyo
Principal Investigator	TANAKA Satoshi 東京大学, 農学生命科学研究科, 准教授 (90242164)
Co-Investigator(Renkei-kenkyūsha)	HATA Kenichiro 独立行政法人国立成育医療研究センター, 周産期病態研究部, 部長 (60360335)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000) Fiscal Year 2014: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000) Fiscal Year 2013: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000) Fiscal Year 2012: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Keywords	栄養膜幹細胞 / LIN28A / RNA結合タンパク質 / TS細胞 / ＬＩＮ２８A / ＲＮＡ結合タンパク質 / ＬＩＮ２８Ａ
Outline of Final Research Achievements	To elucidate a role of LIN28A, an RNA-binding protein, in mouse trophoblast stem cells, we identified LIN28A-binding mRNAs by RIP-seq analysis. The LIN28A-binding mRNAs revealed by the present study were largely different from those identified in human ES cells, implying that a physiological role of LIN28A is different between TS and ES cells. Knockdown of LIN28A only slightly perturbed gene expression profile of TS cells. However, LIN28A KD TS cells mostly died within 24 hours after induction of differentiation. These results suggested that LIN28A is required for cell survival in early phases of trophoblast cell differentiation.