| Project/Area Number |
24249036
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
ANDO Yukio 熊本大学, 生命科学研究部, 教授 (20253742)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Taro 熊本大学, 医学部附属病院, 特任教授 (90381003)
YAMASHITA Satoshi 熊本大学, 大学院生命科学研究部, 准教授 (20457592)
UEDA Mituharu 熊本大学, 医学部附属病院, 講師 (60452885)
OBAYASHI Konen 熊本大学, 医学部附属病院, 特任教授 (90361899)
JONO Hirofumi 熊本大学, 医学部附属病院, 准教授 (40515483)
TASAKI Masayoshi 熊本大学, 大学院生命科学研究部, 助教 (50613402)
神力 悟 熊本大学, 医学部附属病院, その他 (00583048)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥47,970,000 (Direct Cost: ¥36,900,000、Indirect Cost: ¥11,070,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥38,480,000 (Direct Cost: ¥29,600,000、Indirect Cost: ¥8,880,000)
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| Keywords | アミロイドーシス / トランスサイレチン / LC-MS/MS / 家族性アミロイドポリニューロパチー / アミロイド原因蛋白質 / マススペクトロメトリー / ポリニューロパチー家族性アミロイド / ＡＬアミロイドーシス / 遺伝子変異 / レーザーマイクロダイセクション / ALアミロイドーシス |
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| Outline of Final Research Achievements |
To elucidate the usefulness of the analyzing system using LC/ MS MS, both prospective and retrospective studies were performed. To make sure the usefulness of this analyzing system as the retrospective study, we used the formalin fixed cardiac and nerve samples in amyloidosis patients. The coincidence of this analyzing system to the diagnosed type of amyloidosis was 97%. As the prospective study, undetermined samples of amyloidosis suspected patients. Coincidence of this method and clinical diagnosis was 95%. From those results, this analyzing system was found to be useful method for amyloidosis diagnosis. ③To unknown amyloidosis found in the intestine of aged patients, combination of laser microdisection and LC/ MS MS system was applied. By the analysis, Fibrin3 was identified as the precursor protein of the amyloid. This protein was also found to be the component of amyloid body in the brain.
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