Analysis of the relationship between DNA double strand break repair pathway and mutagenesis in response to ionizing radiation by using novel assay systems
| Project/Area Number |
24310038
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | DNA修復 / 体細胞突然変異 / 放射線感受性 / 放射線 / 突然変異 / 遺伝子 / 損傷修復 / DNA損傷修復 / 相同組換え / 放射線影響 |
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| Outline of Final Research Achievements |
DNA double strand break (DSB), which is often induced by ionizing radiation, is one of the most serious DNA damage. To clarify the relationship between DSB repair pathway and mutation induction, we developed a site-specific DSB induction system by using artificial nucleases. We analyzed DSB repair efficiency and mutation spectrum at the DSB site, and found that efficiency and accuracy of DSB repair could be separately regulated. We also found that mutation at FHA domain of NBS1 renders the cells to be sensitive to radiation without increase in mutation frequency. A part of our findings were reported as several papers in scientific journals.
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Report
(5 results)
Research Products
(25 results)
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[Journal Article] Mutations in the FHA-domain of ectopically expressed NBS1 lead to Radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination2014
Author(s)
Maki Ohara, Yumi Funyu, Shunsuke Ebara, Yuki Sakamoto, Ryota Seki, Kenta Iijima, Akiko Ohishi, Junya Kobayashi, Kenshi Komatsu, Akira Tachibana, and Hiroshi Tauchi
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Journal Title
J. Radiat. Res
Volume: 55
Issue: 4
Pages: 690-698
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Analysis of mutation spectrum induced by a site specific DNA double strand break.2015
Author(s)
Sakamoto, K., Kobayashi, H., Kobayashi, K., Honda, C., Iijima, K., kobayashi, J., Komatsu, K., Tachiban, A., Tauchi, H.
Organizer
15th International Congress of Radiation Research (ICRR2015)
Place of Presentation
京都
Year and Date
2015-05-28
Related Report
Int'l Joint Research
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[Presentation] Cell cycle dependence of DNA double strand break repair by homology-directed repair2015
Author(s)
Sakamoto, Y., Okawa, S., Kokuta, T., Teshigahara, A., Iijima, K., Takata, M., Komatsu, K., Tauch, H.
Organizer
15th International Congress of Radiation Research (ICRR2015)
Place of Presentation
京都
Year and Date
2015-05-28
Related Report
Int'l Joint Research
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[Presentation] Modification of camptothecin sensitivity by PARP inhibitor in DNA double strand break repair deficient cell lines
Author(s)
Ohara, M, Tanaka, A., Isaka, S., Tomatsu, S., Takata. M., Komatsu, K., Tatibana, A., Tauchi, H
Organizer
広島大学原爆放射線医科学研究所国際シンポジウム
Place of Presentation
広島
Related Report
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