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Yeast Phenome analysis with super-hidimensional morphological profiling

Research Project

Project/Area Number 24370002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionThe University of Tokyo

Principal Investigator

OHYA Yoshikazu  東京大学, 新領域創成科学研究科, 教授 (20183767)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords出芽酵母 / 表現型解析 / 画像解析 / 細胞形態 / 自然淘汰 / 遺伝子破壊株 / 液泡 / 酸性化 / バイオイメージング / システムズバイオロジー / オーミクス / オルガネラ
Outline of Final Research Achievements

The primary goal of automated morphological analysis of subcellular structures using image extraction and quantification is to ensure objectivity and reproducibility. A well-designed experiment followed by appropriate statistical analyses can provide a wealth of biologically meaningful information. With regard to budding yeast, it is essential to quantitatively study the phenotype of numerous sets of mutants to extract genetically and biologically meaningful information. In this study, we addressed some of the biological findings to which quantitative morphological analysis has contributed significantly.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (28 results)

All 2015 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (13 results) (of which Invited: 6 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Plant-derived antifungal agent poacic acid targets β-1,3-glucan2015

    • Author(s)
      Piotrowski JS, Okada H, Lu F, Li SC, Hinchman L, Ranjan A, Smith DL, Higbee AJ, Ulbrich A, Coon JJ, Deshpande R, Bukhman YV, McIlwain S, Ong IM, Myers CL, Boone C, Landick R, Ralph J, Kabbage M, Ohya Y
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 112 Issue: 12

    • DOI

      10.1073/pnas.1410400112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ohnuki S, Okada H, Ohya Y.2015

    • Author(s)
      Image-based prediction of drug target in yeast.
    • Journal Title

      Methods Mol Biol

      Volume: 1263 Pages: 319-327

    • DOI

      10.1007/978-1-4939-2269-7_24

    • ISBN
      9781493922680, 9781493922697
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Unveiling nonessential gene deletions that confer significant morphological phenotypes beyond natural yeast strains2014

    • Author(s)
      Yang M, Ohnuki S, Ohya Y
    • Journal Title

      BMC Genomics

      Volume: 15 Issue: 1 Pages: 932-932

    • DOI

      10.1186/1471-2164-15-932

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Distinct roles of cell wall biogenesis in yeast morphogenesis as revealed by multivariate analysis of high-dimensional morphometric data.2014

    • Author(s)
      Okada H, Ohnuki S, Roncero C, Konopka JB, Ohya Y
    • Journal Title

      Mol Biol Cell

      Volume: 25 Pages: 222-233

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Distinct roles of cell wall biogenesis in yeast morphogenesis as revealed by multivariate analysis of high-dimensional morphometric data.2014

    • Author(s)
      Okada H, Ohnuki S, Roncero C, Konopka JB, Ohya Y.
    • Journal Title

      Molecular biology of the cell

      Volume: 25(2) Issue: 2 Pages: 222-33

    • DOI

      10.1091/mbc.e13-07-0396

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic changes in brewing yeast cells in culture revealed by statistical analyses of yeast morphological data.2013

    • Author(s)
      Ohnuki S, Enomoto K, Yoshimoto H, Ohya Y.
    • Journal Title

      Journal of bioscience and bioengineering

      Volume: 117(3) Issue: 3 Pages: 278-84

    • DOI

      10.1016/j.jbiosc.2013.08.005

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Organelle acidification is important for localisation of vacuolar proteins in Saccharomyces cerevisiae.2013

    • Author(s)
      Matsumoto R
    • Journal Title

      Protoplasma

      Volume: 250 Issue: 6 Pages: 1283-1293

    • DOI

      10.1007/s00709-013-0510-2

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profilin is required for Ca^<2+> homeostasis and Ca^<2+>-modulated bud formation in yeast.2013

    • Author(s)
      Yoshida M1, Ohnuki S, Yashiroda Y, Ohya Y.
    • Journal Title

      Molecular genetics and genomics

      Volume: 288(7-8) Issue: 7-8 Pages: 317-28

    • DOI

      10.1007/s00438-013-0752-x

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vanillin Inhibits Translation and Induces Messenger Ribonucleoprotein (mRNP) Granule Formation in Saccharomyces cerevisiae: Application and Validation of High-Content, Image-Based Profiling2013

    • Author(s)
      Iwaki A, Ohnuki S, Suga Y, Izawa S and Ohya Y
    • Journal Title

      PLoS ONE

      Volume: 8(4) Issue: 4 Pages: 61748-61748

    • DOI

      10.1371/journal.pone.0061748

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-cell phenomics reveals intra-species variation of phenotypic noise in yeast.2013

    • Author(s)
      Yvert G, Ohnuki S, Nogami S, Imanaga Y, Fehrmann S, Schacherer J. Ohya Y.
    • Journal Title

      BMC systems biology

      Volume: 7 Issue: 1 Pages: 54-54

    • DOI

      10.1186/1752-0509-7-54

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of the biological activity of a novel 24-membered macrolide JBIR-19 in Saccharomyces cerevisiae by the morphological imaging program CalMorph.2012

    • Author(s)
      Shinsuke Ohnuki, et al.
    • Journal Title

      FEMS Yeast Res

      Volume: 12 Pages: 293-304

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] High-Resolution Phenotyping to Elucidate Essential Complex Cellular Processes2014

    • Author(s)
      Yoshikazu Ohya
    • Organizer
      Yeast Phenome Meeting
    • Place of Presentation
      NYU, New York, USA
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Distinct roles of cell wall biogenesis in yeast morphogenesis2014

    • Author(s)
      2) Yoshikazu Ohya
    • Organizer
      The 10th International Mycological Congress
    • Place of Presentation
      Bangkok, Thai
    • Year and Date
      2014-08-04 – 2014-08-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The cell wall contributes to phenotypic robustness in budding yeast2013

    • Author(s)
      Yoshikazu Ohya
    • Organizer
      The 2013 ASCB Annual Meeting
    • Place of Presentation
      New Orleans Ernest N. Morial Convention Center (New Orleas, USA)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 微細藻類の多機能画像解析ソフトウェア2012

    • Author(s)
      大貫 慎輔
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場 マリンメッセ福岡
    • Year and Date
      2012-12-13
    • Related Report
      2012 Annual Research Report
  • [Presentation] 出芽酵母の細胞形態プロファイリングとダイナミクスの解析 : 画像から見えないものを見る2012

    • Author(s)
      大矢 禎一
    • Organizer
      バイオイメージ・インフォマティクスワークショップ2012
    • Place of Presentation
      理化学研究所神戸 発生・再生科学総合研究センター
    • Year and Date
      2012-11-01
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 新規生理活性物質JBIR-19は細胞形態の伸長とアクチンの脱局在を引き起こす2012

    • Author(s)
      大貫 慎輔
    • Organizer
      酵母遺伝学フォーラム第45回研究報告会
    • Place of Presentation
      京都大学宇治キャンパス おうばくプラザ きはだホール
    • Year and Date
      2012-09-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] 出芽酵母の形態形成における細胞壁の各構成成分の役割2012

    • Author(s)
      岡田 啓希
    • Organizer
      酵母遺伝学フォーラム第45回研究報告会
    • Place of Presentation
      京都大学宇治キャンパス おうばくプラザ きはだホール
    • Year and Date
      2012-09-04
    • Related Report
      2012 Annual Research Report
  • [Presentation] 出芽とCa2+恒常性維持の連携機搆2012

    • Author(s)
      吉田 光範
    • Organizer
      酵母遺伝学フォーラム第45回研究報告会
    • Place of Presentation
      京都大学宇治キャンパス おうばくプラザ きはだホール
    • Year and Date
      2012-09-04
    • Related Report
      2012 Annual Research Report
  • [Presentation] Image-based systems biology in the budding yeast Saccharomyces cerevisiae2012

    • Author(s)
      Yoshikazu Ohya
    • Organizer
      ICSB The 13th International Conference on Systems Biology
    • Place of Presentation
      Toronto University (Toronto, CANADA)
    • Year and Date
      2012-08-21
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Chemical-genetic approaches for the elucidation of cell wall biogenesis in Saccharomyces cerevisiae2012

    • Author(s)
      Shinsuke Ohnuki
    • Organizer
      ICSB The 13th International Conference on Systems Biology
    • Place of Presentation
      Toronto University (Toronto, CANADA)
    • Year and Date
      2012-08-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] Profilin is required for Ca (2+) homeostasis and bud formation2012

    • Author(s)
      Mitsunori Yoshida
    • Organizer
      2012 Yeast Genetics and Molecular Biology Meeting
    • Place of Presentation
      Princeton University (Princeton, USA)
    • Year and Date
      2012-08-03
    • Related Report
      2012 Annual Research Report
  • [Presentation] Multi-target drugs induce complex morphological changes in the budding yeast Saccharomyces cerevisiae.2012

    • Author(s)
      Shinsuke Ohnuki
    • Organizer
      2012 Yeast Genetics and Molecular Biology Meeting
    • Place of Presentation
      Princeton University (Princeton, USA)
    • Year and Date
      2012-08-01
    • Related Report
      2012 Annual Research Report
  • [Presentation] Chemical-genetic analysis on the elucidation of cell wall biogenesis in Saccharomyces cerevisiae2012

    • Author(s)
      Yoshikazu Ohya
    • Organizer
      5th International Conference on Molecular Mecha-nisms of Fungal Cell Wall Biogenesis
    • Place of Presentation
      Hotel Zora, Kravata Conference Hall (CROATIA)
    • Year and Date
      2012-06-08
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks] 生命応答システム分野

    • URL

      http://ps.k.u-tokyo.ac.jp/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 生命応答システム分野

    • URL

      http://ps.k.u-tokyo.ac.jp/

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://ps.k.u-tokyo.ac.jp/

    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞観察装置、細胞観察方法及びそのプログラム2012

    • Inventor(s)
      大矢禎一他
    • Industrial Property Rights Holder
      JST
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-11-28
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

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