Analysis of evolution of sexual reproductive system in Closterium peracerosum-strigosum-littorale complex, comparing sex-chromosome like regions

• Project/Area Number: 24370038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Women's University
• Principal Investigator: Sekimoto Hiroyuki 日本女子大学, 理学部, 教授 (20281652)
• Co-Investigator(Kenkyū-buntansha): 西山 智明 金沢大学, 学際科学実験センター, 助教 (50390688)
• Co-Investigator(Renkei-kenkyūsha): ABE Jun 日本女子大学, 理学部, 学術研究員 (10424764) ; TSUCHIKANE Yuki 日本女子大学, 理学部, 助教 (20434152)
• Research Collaborator: KAWAI Junko 日本女子大学, 理学部, 学術研究員 (30727673) ; OOTSUKI Ryo 日本女子大学, 理学部, 学術研究員 (10646962) ; KOMIYA Ayumi ; FUJIWARA Anri ; KANDA Naho ; KANAZAWA Manaki
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: ミカヅキモ / 性決定遺伝子 / 性染色体 / 有性生殖 / 生殖様式 / 性決定 / 進化 / 性フェロモン / ゲノム

Outline of Final Research Achievements

We focused on Closterium peracerosum-strigosum-littorale complex (C. psl. complex), which is one of the unicellular charophycean algae. They have two sexes: mating type plus (mt+) and mating type minus (mt-). However, the mechanism of sex determination had not been clarified. In this study, we found a gene, which was highly linked to mt- phenotype of the progenies. It encoded a putative transcription factor and was named CpMinus1. To evaluate the function, a construct for ectopic expression was prepared and introduced into mt+ strain. Among 6 stable transformants, 5 showed sexual reaction without mating partners, like as the case of homothallic strains. Two transformants (R5 and R6) tended to form zygotes with mt+ strain but not with mt- strain. The gene expression profile of strain R5 was drastically changed to that of mt- strains. We concluded that the CpMinus1 gene was responsible for both the expression of mt- phenotype and the suppression of mt+ phenotype in C. psl. complex.

Research Products

• [Journal Article] Preparation of knockdown transformants of unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. (2016)
  • Author(s): Abe, J., Hirano, N., Komiya, A., Kanda, N., Fujiwara, A., Hori, S., Tsuchikane, Y., Sekimoto, H.
  • Journal Title: Bio-protocol Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A receptor-like kinase, related with cell wall sensor of higher plants, is required for sexual reproduction in the unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. (2015)
  • Author(s): Hirano, N., Marukawa, Y., Abe, J., Hashiba, S., Ichikawa, M., Tanabe, Y., Ito, M., Nishii, I., Tsuchikane, Y., Sekimoto, H.
  • Journal Title: Plant Cell Physiol. Volume: 56 Issue: 7 Pages: 1456-1462
  • DOI: 10.1093/pcp/pcv065
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The algal ancestor of land plants was pre-adapted for symbiosis. (2015)
  • Author(s): Delaux, P-M., Radhakrishnan, G.V., Jayaraman, D., Cheema, J., Malbreil, M., Volkening, J.D., Sekimoto, H., Nishiyama, T., Melkonian, M., Pokorny, L., Rothfels, C.J., Sederoff, H.W., Stevenson, D.W., Surek, B., Zhang, Y., Sussman, M.R., Dunand, C., Morris, R.J., Roux, C., Wong, G.K-S., Oldroyd, G.E.D., Ané, J-M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 112 Issue: 43 Pages: 13390-13395
  • DOI: 10.1073/pnas.1515426112
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Receptor-like kinase, related with cell wall sensor of higher plants, is required for sexual reproduction in the unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. (2015)
  • Author(s): Hirano, N., Marukawa, Y., Abe, J., Hashiba, S., Ichikawa, M., Tanabe, Y., Ito, M., Nishii, I., Tsuchikane, Y., Sekimoto, H.
  • Journal Title: Plant Cell Physiol. Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization of sexual reproductive processes in Chara braunii (Charales, Charophyceae). (2014)
  • Author(s): Sato, M., Sakayama, H., Sato, M., Ito, M., Sekimoto, H.
  • Journal Title: Phycol. Res. Volume: 62 Issue: 3 Pages: 214-221
  • DOI: 10.1111/pre.12056
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Detailed analyses on the parthenospore formation in Closterium moniliferum (Zygnematophyceae, Charophyta). (2014)
  • Author(s): Yuki Tsuchikane, Ayaka Nakai, and Hiroyuki Sekimoto
  • Journal Title: Phycologia. Volume: 53 Issue: 6 Pages: 571-578
  • DOI: 10.2216/14-35r1.1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The evolution of male-female sexual dimorphism predates the gender-based divergence of the mating locus gene MAT3/RB. (2013)
  • Author(s): Rintaro Hiraide
  • Journal Title: Molecular Biology and Evolution Volume: 30 Issue: 5 Pages: 1038-1040
  • DOI: 10.1093/molbev/mst018
  • Peer Reviewed
• [Journal Article] Properties of cell surface carbohydrates in sexual reproduction of the Closterium peracerosum-strigosum-littorale complex (Zygnematophyceae, Charophyta) (2012)
  • Author(s): Hori, S., Sekimoto, H., Abe, J
  • Journal Title: Phycol. Res Volume: 60 Issue: 4 Pages: 254-260
  • DOI: 10.1111/j.1440-1835.2012.00656.x
  • NAID: 10031136641
  • Peer Reviewed
• [Journal Article] New insights into the regulation of sexual reproduction in Closterium (2012)
  • Author(s): Sekimoto, H., Abe, J., Tsuchikane, Y.
  • Journal Title: The international review of Cell and Molecular Biology Volume: 297 Pages: 309-338
  • DOI: 10.1016/b978-0-12-394308-8.00014-5
  • ISBN: 9780123943088
  • Peer Reviewed
• [Journal Article] ミカヅキモの生殖について (2012)
  • Author(s): 土金勇樹、関本弘之
  • Journal Title: 海洋と生物 Volume: 34 Pages: 426-434
• [Journal Article] 単細胞シャジクモ藻類ミカヅキモにおける生殖隔離、種分化機構の研究 (2012)
  • Author(s): 関本弘之
  • Journal Title: IFO Research communications Volume: 26 Pages: 71-85
• [Presentation] ヒメミカヅキモにおける組換え型性フェロモンを用いた生殖隔離障壁の解析 (2016)
  • Author(s): 土金勇樹、吉田睦未、関本弘之
  • Organizer: 日本藻類学会第40回大会
  • Place of Presentation: 日本歯科大学（東京都千代田区）
  • Year and Date: 2016-03-20
• [Presentation] CpMinus1遺伝子はヒメミカヅキモの性を決定する (2015)
  • Author(s): 小宮 あゆみ,阿部 淳,川井 絢子,鈴木 穣,豊田 敦,藤山 秋佐夫,大槻 涼,土金 勇樹, 西山 智明,関本 弘之
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ（新潟市）
  • Year and Date: 2015-09-08
• [Presentation] 接合藻ヒメミカヅキモにおける新規交配群 G の発見とトランスクリプトーム解析 (2015)
  • Author(s): 土金 勇樹,渡邊 樹梨,加藤 万智,鈴木 穣,西山 智明,関本 弘之
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ（新潟市）
  • Year and Date: 2015-09-08
• [Presentation] シャジクモ藻類ヒメミカヅキモには、２つのクラスのKNOX遺伝子が存在する (2015)
  • Author(s): 金澤愛樹、坂本季美枝、鈴木穣、豊田敦、藤山秋佐夫、西山智明、関本弘之
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ（新潟市）
  • Year and Date: 2015-09-08
• [Presentation] ヒメミカヅキモの受容体型タンパク質CpRLP1の機能解析 (2015)
  • Author(s): 神田奈保,市川真知子,小野彩花,土金勇樹,関本弘之
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ（新潟市）
  • Year and Date: 2015-09-07
• [Presentation] Sexual reproduction and sex determination of green algae (2015)
  • Author(s): Hiroyuki Sekimoto
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ（新潟市）
  • Year and Date: 2015-09-07
  • Invited
• [Presentation] The CpMinus1 gene, specifically localized on the mating-type minus genome, is responsible for the sex determination of heterothallic Closterium peracerosum-strigosum-littorale complex (2015)
  • Author(s): Komiya, A., Abe, J., Kawai, J., Suzuki, Y., Ootsuki, R., Tsuchikane, Y., Nishiyama, T., Sekimoto, H.
  • Organizer: 6th European Phycological Congress
  • Place of Presentation: London, England
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] Characterization of a sex-specific receptor-like protein, expressing during the conjugation of heterothallic Closterium peracerosum-strigosum-littorale complex (2015)
  • Author(s): Kanda, N., Ichikawa, M., Ono, A., Tsuchikane, Y., Abe, J., Sekimoto, H.
  • Organizer: 6th European Phycological Congress
  • Place of Presentation: London, England
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] A receptor-like kinase, related with cell wall sensor of higher plants, is responsible for sexual reproduction in the unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex (2015)
  • Author(s): Sekimoto, H.
  • Organizer: 6th European Phycological Congress
  • Place of Presentation: London, England
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] ホモタリックなヒメミカヅキモにおけるCpMinus1相同遺伝子の機能解析 (2015)
  • Author(s): 土金勇樹、横山佳奈、鈴木穣、西山智明、関本弘之
  • Organizer: 日本藻類学会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] 自殖するミカヅキモにおける性決定遺伝子CpMinus1の機能解析 (2015)
  • Author(s): 横山佳奈、関本弘之、鈴木穣、西山智明、土金勇樹
  • Organizer: 日本植物分類学会
  • Place of Presentation: 福島大学（福島県福島市）
  • Year and Date: 2015-03-06 – 2015-03-08
• [Presentation] ヤブソテツにおける葉緑体全長配列の解読 (2015)
  • Author(s): 大槻涼、関本弘之、三澤計治
  • Organizer: 日本植物分類学会
  • Place of Presentation: 福島大学（福島県福島市）
  • Year and Date: 2015-03-06 – 2015-03-08
• [Presentation] ヒメミカヅキモの－型細胞ゲノム特異的遺伝子CpMinus1の機能解析 (2014)
  • Author(s): 小宮あゆみ、阿部淳、川井絢子、鈴木穣、豊田敦、藤山秋佐夫、大槻涼、土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 植物細胞リン酸輸送機構とその進化について (2014)
  • Author(s): 藤原ひとみ、大西美輪、坂山英俊、石崎公庸、豊倉浩一、関本弘之、西山智明、七條千津子、小菅桂子、深城英弘、三村徹郎
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] ヒメミカヅキモの性フェロモンと結合するファシクリンIタンパク質の機能解析 (2014)
  • Author(s): 市原健介、滝口若菜、原真由美、土金勇樹、阿部淳、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 接合藻ヒメミカヅキモにおけるホモタリズムの進化 (2014)
  • Author(s): 土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] シャジクモ藻類ヒメミカヅキモにおける陸上植物特異的な転写因子の探索と発現解析 (2014)
  • Author(s): 川井絢子、阿部淳、鈴木穣、豊田敦、藤山秋佐夫、土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学（神奈川県川崎市）
  • Year and Date: 2014-09-12
• [Presentation] ヒメミカヅキモの－型細胞特異的遺伝子CpMinus1の解析 (2014)
  • Author(s): 小宮あゆみ、阿部淳、川井絢子、鈴木穣、豊田敦、藤山秋佐夫、大槻涼、土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学
• [Presentation] 植物細胞リン酸輸送機構とその進化について (2014)
  • Author(s): 藤原ひとみ、大西美輪、坂山英俊、石崎公庸、関本弘之、西山智明、七條千津子、深城英弘、三村徹郎
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学
• [Presentation] 生殖隔離に関わる性フェロモンのゲノムレベルでの特徴 (2013)
  • Author(s): 土金勇樹、西山智明、関本弘之
  • Organizer: 日本藻類学会
  • Place of Presentation: 山梨大学
  • Year and Date: 2013-03-28
• [Presentation] A receptor-like kinase responsible for the progress of sexual reproduction of a charophycean alga, Closterium peracerosum-strigosum-littorale complex (2013)
  • Author(s): Sekimoto, H., Hirano, N., Marukawa, Y., Abe, J., Tsuchikane, Y.
  • Organizer: 10th International phycological congress
  • Place of Presentation: Orlando, Florida USA
• [Presentation] Characterization of a conjugation processes in homothallic Closterium peracerosum-strigosum-littorale complex (2013)
  • Author(s): Tsuchikane, Y., Sekimoto, H
  • Organizer: 10th International phycological congress
  • Place of Presentation: Orlando, Florida USA
• [Presentation] ヒメミカヅキモの有性生殖に関わる受容体型キナーゼCpRLK1の生理機能 (2013)
  • Author(s): 平野直子、土金勇樹、丸川祐佳、阿部淳、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 北海道大学
• [Presentation] シャジクモ藻類ヒメミカヅキモの有性生殖制御 (2013)
  • Author(s): 関本弘之、平野直子、大槻涼、川井絢子、土金勇樹、西山智明、阿部淳
  • Organizer: 日本植物学会
  • Place of Presentation: 北海道大学
  • Invited
• [Presentation] 同型配偶子接合を行うヒメミカヅキモの接合過程における性特異的な挙動の探索 (2013)
  • Author(s): 田中優希、関本弘之、土金勇樹
  • Organizer: 日本植物学会
  • Place of Presentation: 北海道大学
• [Presentation] 接合藻ジュズミカヅキモ（Closterium moniliferum）における単為胞子形成機構 (2013)
  • Author(s): 土金勇樹、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 北海道大学
• [Presentation] シャジクモ藻類ヒメミカヅキモの有性生殖機構 (2013)
  • Author(s): 関本弘之
  • Organizer: 日本動物学会
  • Place of Presentation: 岡山大学
  • Invited
• [Presentation] Intercellular communication in sexual reproduction of a unicellular charaphycean alga, Closterium peracerosum-strigosum-littorale comple (2012)
  • Author(s): Sekimoto, H., Hirano, N., Marukawa, Y., Tsuchikane, Y., Abe, J.
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: 名古屋ガーデンパレス(名古屋市)(招待講演)
  • Year and Date: 2012-11-16
• [Presentation] Sexual recognition between homothallic and heterothallic strains of Closterium (2012)
  • Author(s): Tsuchikane, Y., Sekimoto, H
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: 名古屋ガーデンパレス(名古屋市)
  • Year and Date: 2012-11-15
• [Presentation] A novel receptor-like protein kinase, CpRLKl, involved in the sexual rep roduction of a unicellular charophycean alga, Closteriurm peracerosum-strigosum-littorale complex (2012)
  • Author(s): Hirano, N., Marukawa, Y., Hashiba, S., Tsuchikane, Y., Abe, J., Sekimoto, H.
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: 名古屋ガーデンパレス(名古屋市)
  • Year and Date: 2012-11-14
• [Presentation] 同型配偶子接合を行うヒメミカヅキモの接合過程を追う (2012)
  • Author(s): 田中優希、土金勇樹、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2012-09-15
• [Presentation] ヒメミカヅキモの有性生殖に関わる受容体型タンパク質キナーゼCpRLKlの特性解析 (2012)
  • Author(s): 平野直子、橋場彩有里、土金勇樹、阿部淳、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2012-09-15
• [Presentation] ヒメミカヅキモの性フェロモンPR-IP Inducer結合分子の特性解析 (2012)
  • Author(s): 関本弘之、滝口若菜、原真由美、高橋紗也香、土金勇樹、阿部淳
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2012-09-15
• [Presentation] 接合藻ヒメミカヅキモにおける生殖隔離に関わる性フェロモンの進化 (2012)
  • Author(s): 土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2012-09-15
• [Presentation] 接合藻ミカヅキモ(Closterium moniliferunisにおける単為胞子形成株の生理学的解析 (2012)
  • Author(s): 土金勇樹、中井彩香、関本弘之
  • Organizer: 日本藻類学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2012-07-15
• [Presentation] カヅキモの有性生殖に関わる細胞間情報伝達機構の解析 (2012)
  • Author(s): 関本弘之
  • Organizer: 化学生態学研究会
  • Place of Presentation: 湯の川プリンスホテル渚亭(函館市(招待講演)
  • Year and Date: 2012-06-30
• [Book] 動植物の受精学 (2014)
  • Author(s): 野崎久義、関本弘之
  • Total Pages: 333
  • Publisher: 化学同人
• [Book] Reproductive Biology of Plants (2014)
  • Author(s): Sekimoto, H., Abe, J., Tsuchikane, Y.
  • Total Pages: 380
  • Publisher: CRC press.
• [Book] Reproductive Biology of Plants (2014)
  • Author(s): Sekimoto, H., Abe, J., Tsuchikane, Y.
  • Publisher: CRC press
• [Book] Sexual Reproduction in Animals and Plants (2014)
  • Author(s): Sekimoto, H., Tsuchikane, Y., Abe, J.
  • Total Pages: 480
  • Publisher: Springer
• [Book] 生物の受精(澤田均他編著)、「第4章 藻類の有性生殖」を分担執筆 (2013)
  • Author(s): 野崎久義、関本弘之
  • Publisher: 化学同人(印刷中)
• [Book] 藻類ガイドブック(渡辺信他 編著)、「性フェロモン」を分担執筆 (2012)
  • Author(s): 関本弘之
  • Total Pages: 768
  • Publisher: NTS