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Establishment of cerebellar connectome

Research Project

Project/Area Number 24370088
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Developmental biology
Research InstitutionNagoya University

Principal Investigator

Hibi Masahiko  名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)

Co-Investigator(Renkei-kenkyūsha) TAKEUCHI MIKI  名古屋大学, 生物機能開発利用研究センター, 研究員 (60625127)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords小脳 / 神経回路形成 / ゼブラフィッシュ / 蛍光タンパク質 / 可視化 / ライブイメージング / 変異体 / 神経発生
Outline of Final Research Achievements

We isolated transgenic zebrafish lines that express a modified version of Gal4 in the cerebellar neural circuits: granule, Purkinje, eurydendroid cells, Bergmann glia, and or the neurons in the inferior olive nuclei. Using these lines, we investigated the anatomy and developmental processes of the cerebellar neural circuitry. Identification of the locus of the zebrafish mutant shiomaneki, which displays aberrant axons of granule cells, revealed that type IV collagen controls the formation of granule cell axons by regulating the integrity of the basement membrane. Analysis of the medaka mutant ro, which shows abnormal swimming behavior, revealed that Contactin1 plays a conserved role in the formation and/or function of the neural network involved in motor coordination. We carried out transcriptome analysis of cerebellar neurons to identify molecules controlling the cerebellar neural circuit formation.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (32 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (20 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (2 results) Remarks (1 results)

  • [Journal Article] ype IV collagen controls the axogenesis of cereberr granule cells by regulating basement membrane integrity in zebrafish.2015

    • Author(s)
      Takeuchi, M., Yamaguchi, S., Yonemura, S., Kakiguchi, K., Sato, Y., Higashiyama, T., Shimizu, T., and, Hibi, M.
    • Journal Title

      PLoS genet.

      Volume: 11 Issue: 10 Pages: e1005587-e1005587

    • DOI

      10.1371/journal.pgen.1005587

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination.2015

    • Author(s)
      Kim, J.D., Park, K.E., Ishida, J., Kako, K., Hamada, J., Kani, S., Takeuchi, M., Namiki, K., Fukui, H., Fukuhara, S., Hibi, M,. Kobayashi, M., Kanaho, Y., Kasuya, Y., Mochizuki, N., Fukamizu, A.
    • Journal Title

      Sci. Adv.

      Volume: 1 Issue: 11

    • DOI

      10.1126/sciadv.1500615

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry2015

    • Author(s)
      Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., and Hibi, M.
    • Journal Title

      Developmental Biology

      Volume: 397 Issue: 1 Pages: 1-17

    • DOI

      10.1016/j.ydbio.2014.09.030

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sox5 functions as a fate switch in medaka pigment cell development.2014

    • Author(s)
      Nagao Y, Suzuki T, Shimizu A, Kimura T, Seki R, Adachi T, Inoue C, Omae Y, Kamei Y, Hara I, Taniguchi Y, Naruse K, Wakamatsu Y, Kelsh RN, Hibi M, Hashimoto H
    • Journal Title

      PLoS genetics

      Volume: 10(4) Issue: 4 Pages: e1004246-e1004246

    • DOI

      10.1371/journal.pgen.1004246

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish2013

    • Author(s)
      Kwon HB, Fukuhara S, Asakawa K, Ando K, Kashiwada T, Kawakami K Hibi M, Kwon YG, Kim KW, Alitalo K, Mochizuki N.
    • Journal Title

      Development

      Volume: 140 Issue: 19 Pages: 4081-4090

    • DOI

      10.1242/dev.091702

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Requirement of zebrafish ataxin-7 in differentiation of photoreceptors and cerebellar neurons.2012

    • Author(s)
      Yanicostas, C.
    • Journal Title

      PLoS One

      Volume: 7 Issue: 11 Pages: e50705-e50705

    • DOI

      10.1371/journal.pone.0050705

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish.2012

    • Author(s)
      Tran, L.D.
    • Journal Title

      Development

      Volume: 139 Issue: 19 Pages: 3644-3652

    • DOI

      10.1242/dev.082362

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lesion-induced generation of interneuron cell types in specific dorso-ventral domains in the spinal cord of adult zebrafish2012

    • Author(s)
      Kuscha, V., Fraser, S. L., Dias, T. B., Hibi, M., Becker, T., and Becker, C. G.
    • Journal Title

      J. Comp. Neurol

      Volume: (in press) Issue: 16 Pages: 3604-3616

    • DOI

      10.1002/cne.23115

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution.2012

    • Author(s)
      Yuuta Moriyama
    • Journal Title

      Current Biology

      Volume: 21 Issue: 7 Pages: 601-607

    • DOI

      10.1016/j.cub.2012.01.063

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 真骨魚類に見る小脳の進化と発生2016

    • Author(s)
      日比正彦、竹内未紀、山口信悟*、松田光司*、原雄一郎、榊原考将、工樂樹洋、吉田将之、清水貴史
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      郡山
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Transcriptome analysis of granule cells and Purkinje cells in zebrafish cerebellum.2015

    • Author(s)
      Takeuchi M, Yamaguchi S, Hayashi T, Sakakibara Y, Matsuda K, Hara Y, Hayashi T, Kuraku S, Shimizu T, Hibi M.
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Evolution and development of cerebellar neural circuitry in teleosts.2015

    • Author(s)
      Hibi M, Takeuchi M, Yamaguchi S, Matsuda K, Hayashi T, Hara Y, Hayashi T, Sakakibara Y, Yoshida M, Kuraku S, Shimizu T.
    • Organizer
      第48回日本発生生物学会大会
    • Place of Presentation
      つくば
    • Year and Date
      2015-06-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Integrity of basement membrane is important for axogenesis of cerebellar granule cells.2015

    • Author(s)
      Takeuchi M, Yamaguchi S, Hayashi T, Yonemura S, Hara Y, Hayashi T, Kuraku S, Shimizu T, Hibi M.
    • Organizer
      第48回日本発生生物学会大会
    • Place of Presentation
      つくば
    • Year and Date
      2015-06-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Formation and function of cerebellar neural circuitry.2015

    • Author(s)
      Hibi M.
    • Organizer
      Zebrafish Systems Biology Workshop
    • Place of Presentation
      Jaenlia Research Campus, Virginia
    • Year and Date
      2015-04-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Precise assembly of the basement membrane is important for the axogenesis of cerebellar granule cells.2014

    • Author(s)
      Takeuchi M, Yamaguchi S, Yonemura S, Asakawa K, Kawakami K, Takada S, Shimizu T, Hibi M.
    • Organizer
      第20回小型魚類研究会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-20 – 2014-09-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Basement membrane structure is important for axogenesis of cerebellar granule cells.2014

    • Author(s)
      Takeuchi M, Yonemura S, Asakawa K, Kawakami K, Takada S, Kuraku S, Hayashi T, Yamaguchi S, Shimizu T, Hibi M.
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Control of axon guidance of cerebellar granule cells by the basement membrane.2014

    • Author(s)
      Takeuchi M, Hayashi T, Yamaguchi S, Yonemura S, Asakawa K, Kawakami K, Takada S, Kuraku S, Shimizu T, Hibi M.
    • Organizer
      11th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Wisconsin, USA
    • Year and Date
      2014-06-24 – 2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dissection of cerebellar neural circuitry with zebrafish Gal4 gene/enhancer trap lines.2014

    • Author(s)
      Matsuda K, Takeuchi M, Asakawa K, Ohkura M, Miyasaka N, Yoshihara Y, Hayashi T, Kuraku S, Kawakami K, Hibi M, Shimizu T.
    • Organizer
      第47回日本発生生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Precise axogenesis of the cerebellar granule cells requires interaction between their axons and the basement membrane.2014

    • Author(s)
      Takeuchi M, Yonemura S, Asakawa K, Kawakami K, Takada S, Shimizu T, Hibi M.
    • Organizer
      第47回日本発生生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Microtubule-Dependent Dorsal Determination in zebrafish.2013

    • Author(s)
      日比 正彦
    • Organizer
      CDB symposium 2013
    • Place of Presentation
      理化学研究所(兵庫県)(招待講演)
    • Year and Date
      2013-03-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of cerebellar granule cell axons in zebrafish.2013

    • Author(s)
      Yamaguchi S, Takeuchi M, Asakawa K, Kawakami K, Shimizu T, and Hibi M.
    • Organizer
      第46回日本発生生物学会大会
    • Place of Presentation
      松江
    • Related Report
      2013 Annual Research Report
  • [Presentation] Basement membrane integrity is required for axogenesis of cerebellar granule cells.2013

    • Author(s)
      Takeuchi M, Yonemura S, Asakawa K, Kawakami K, Takada S, Shimizu T, Hibi M.
    • Organizer
      第46回日本発生生物学会大会
    • Place of Presentation
      松江
    • Related Report
      2013 Annual Research Report
  • [Presentation] 真骨魚類における小脳神経回路形成2012

    • Author(s)
      日比 正彦
    • Organizer
      総合研究大学院大学融合「脳の進化」研究会
    • Place of Presentation
      総合研究大学院大学(神奈川県)(招待講演)
    • Year and Date
      2012-11-13
    • Related Report
      2012 Annual Research Report
  • [Presentation] Formation of cerebellar neural circuits in zebrafish.2012

    • Author(s)
      日比 正彦
    • Organizer
      Swiss-Japanese Developmental Biology Meeting
    • Place of Presentation
      京都ガーデンパレス(京都府)
    • Year and Date
      2012-11-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] Genetic control for neural circuit formation in teleost cerebellum.2012

    • Author(s)
      日比 正彦
    • Organizer
      第45回日本発生生物学会合同大会
    • Place of Presentation
      神戸国際会議場(兵庫県)(招待講演)
    • Year and Date
      2012-05-31
    • Related Report
      2012 Annual Research Report
  • [Presentation] Role of basement membrane in axogenesis of cerebellar granule cells in zebrafish2012

    • Author(s)
      日比 正彦
    • Organizer
      Asia-Pacific Developmental Biology Conference
    • Place of Presentation
      台北(台湾)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Deciphering cerebellar neural circuitry using the zebrafish model.

    • Author(s)
      Hibi M, Takeuchi M, Yamaguchi S, Matsuda K, Yonemura S, Asakawa K, Kawakami K, and Shimizu T.
    • Organizer
      高等研プロジェクト会議「ゲノム工学とイメージングサイエンスに基づく生命システム研究の新展開」
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of functional cerebellar neural circuitry in zebrafish.

    • Author(s)
      Hibi M, Takeuchi M, Yamaguchi S, Matsuda K, Yonemura S, Asakawa K, Kawakami K, and Shimizu T.
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of cerebellar neural circuitry.

    • Author(s)
      Hibi M.
    • Organizer
      Janelia Workshop on Zebrafish Genetics, Transgenesis, and Systems Biology
    • Place of Presentation
      Ashburn, VA, USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] "Deciphering Cerebellar Neural Circuitry Involved in Higher Order Functions" in New Principles in Developmental Processes.2014

    • Author(s)
      Hibi M, Shimizu T.
    • Total Pages
      24
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] New Principles in Developmental Processes, Chapter 13 Deciphering Cerebellar Neural Circuitry Involved in Higher Order Functions Using the Zebrafish Model2014

    • Author(s)
      Hibi M, Shimizu T.
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Remarks] 名古屋大学生物機能開発利用研究センター動物器官機能研究分野

    • URL

      http://bbc.agr.nagoya-u.ac.jp/~junkei/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

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