Establishment of cerebellar connectome
Project/Area Number |
24370088
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Developmental biology
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Research Institution | Nagoya University |
Principal Investigator |
Hibi Masahiko 名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)
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Co-Investigator(Renkei-kenkyūsha) |
TAKEUCHI MIKI 名古屋大学, 生物機能開発利用研究センター, 研究員 (60625127)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
|
Keywords | 小脳 / 神経回路形成 / ゼブラフィッシュ / 蛍光タンパク質 / 可視化 / ライブイメージング / 変異体 / 神経発生 |
Outline of Final Research Achievements |
We isolated transgenic zebrafish lines that express a modified version of Gal4 in the cerebellar neural circuits: granule, Purkinje, eurydendroid cells, Bergmann glia, and or the neurons in the inferior olive nuclei. Using these lines, we investigated the anatomy and developmental processes of the cerebellar neural circuitry. Identification of the locus of the zebrafish mutant shiomaneki, which displays aberrant axons of granule cells, revealed that type IV collagen controls the formation of granule cell axons by regulating the integrity of the basement membrane. Analysis of the medaka mutant ro, which shows abnormal swimming behavior, revealed that Contactin1 plays a conserved role in the formation and/or function of the neural network involved in motor coordination. We carried out transcriptome analysis of cerebellar neurons to identify molecules controlling the cerebellar neural circuit formation.
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Report
(5 results)
Research Products
(32 results)
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[Journal Article] ype IV collagen controls the axogenesis of cereberr granule cells by regulating basement membrane integrity in zebrafish.2015
Author(s)
Takeuchi, M., Yamaguchi, S., Yonemura, S., Kakiguchi, K., Sato, Y., Higashiyama, T., Shimizu, T., and, Hibi, M.
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Journal Title
PLoS genet.
Volume: 11
Issue: 10
Pages: e1005587-e1005587
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination.2015
Author(s)
Kim, J.D., Park, K.E., Ishida, J., Kako, K., Hamada, J., Kani, S., Takeuchi, M., Namiki, K., Fukui, H., Fukuhara, S., Hibi, M,. Kobayashi, M., Kanaho, Y., Kasuya, Y., Mochizuki, N., Fukamizu, A.
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Journal Title
Sci. Adv.
Volume: 1
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry2015
Author(s)
Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., and Hibi, M.
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Journal Title
Developmental Biology
Volume: 397
Issue: 1
Pages: 1-17
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Sox5 functions as a fate switch in medaka pigment cell development.2014
Author(s)
Nagao Y, Suzuki T, Shimizu A, Kimura T, Seki R, Adachi T, Inoue C, Omae Y, Kamei Y, Hara I, Taniguchi Y, Naruse K, Wakamatsu Y, Kelsh RN, Hibi M, Hashimoto H
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Journal Title
PLoS genetics
Volume: 10(4)
Issue: 4
Pages: e1004246-e1004246
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Lesion-induced generation of interneuron cell types in specific dorso-ventral domains in the spinal cord of adult zebrafish2012
Author(s)
Kuscha, V., Fraser, S. L., Dias, T. B., Hibi, M., Becker, T., and Becker, C. G.
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Journal Title
J. Comp. Neurol
Volume: (in press)
Issue: 16
Pages: 3604-3616
DOI
Related Report
Peer Reviewed
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[Presentation] Evolution and development of cerebellar neural circuitry in teleosts.2015
Author(s)
Hibi M, Takeuchi M, Yamaguchi S, Matsuda K, Hayashi T, Hara Y, Hayashi T, Sakakibara Y, Yoshida M, Kuraku S, Shimizu T.
Organizer
第48回日本発生生物学会大会
Place of Presentation
つくば
Year and Date
2015-06-02
Related Report
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[Presentation] Control of axon guidance of cerebellar granule cells by the basement membrane.2014
Author(s)
Takeuchi M, Hayashi T, Yamaguchi S, Yonemura S, Asakawa K, Kawakami K, Takada S, Kuraku S, Shimizu T, Hibi M.
Organizer
11th International Conference on Zebrafish Development and Genetics
Place of Presentation
Wisconsin, USA
Year and Date
2014-06-24 – 2014-06-28
Related Report
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[Presentation] Dissection of cerebellar neural circuitry with zebrafish Gal4 gene/enhancer trap lines.2014
Author(s)
Matsuda K, Takeuchi M, Asakawa K, Ohkura M, Miyasaka N, Yoshihara Y, Hayashi T, Kuraku S, Kawakami K, Hibi M, Shimizu T.
Organizer
第47回日本発生生物学会大会
Place of Presentation
名古屋
Year and Date
2014-05-27 – 2014-05-30
Related Report
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