DIfferent mechanisms of self-incompatibility in Rosaceae: non-self recognition by multiple factors type ans self recognition by single factor type

• Project/Area Number: 24380004
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Breeding science
• Research Institution: Chiba University
• Principal Investigator: SASSA Hidenori 千葉大学, 園芸学研究科, 准教授 (50295507)
• Co-Investigator(Kenkyū-buntansha): 牛島 幸一郎 岡山大学, 環境生命科学研究科, 准教授 (20379720)
• Project Period (FY): 2012-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 自家不和合性 / バラ科 / 花粉 / 雌ずい / RNase / F-box / S-RNase

Outline of Final Research Achievements

Pollen-expressed apple homologs of SBP1 and SSK1 were cloned and named MdSBP1 and MdSSK1, respectively, and subjected to further analyses. Both MdSBP1 and MdSSK1 interacted with SFBB. Further analyses showed that interaction of MdSSK1 with SFBB is stronger than that of MdSBP1, and that mRNA of MdSSK1 is much abundant than MdSBP1. Together, the results suggest that MdSSK1 containing SCFSFBB complex would play a major role in self-incompatibility in apple.

Research Products

• [Journal Article] Molecular mechanism of the S-RNase-based gametophytic self-incompatibility in fruit trees of Rosaceae (2016)
  • Author(s): Sassa, H.
  • Journal Title: Breeding Science Volume: 66 Issue: 1 Pages: 116-121
  • DOI: 10.1270/jsbbs.66.116
  • NAID: 130005141824
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of SFBB-containing canonical and noncanonical SCF complexes in pollen of apple (Malus × domestica). (2014)
  • Author(s): Minamikawa, F. M., Koyano, R., Kikuchi, S., Koba, T. and Sassa, H.
  • Journal Title: PLoS ONE Volume: 9 Issue: 5 Pages: e97642-e97642
  • DOI: 10.1371/journal.pone.0097642
  • Peer Reviewed / Open Access
• [Journal Article] Seed formation in triploid loquat (<i>Eriobotrya japonica</i>) through cross-hybridization with pollen of diploid cultivars (2014)
  • Author(s): Kikuchi, S., Iwasuna, M., Kobori, A., Tsutaki, Y., Yoshida, A., Murota, Y., Nishino, E., Sassa, H. and Koba, T.
  • Journal Title: Breeding Science Volume: 64 Issue: 2 Pages: 176-182
  • DOI: 10.1270/jsbbs.64.176
  • NAID: 130004146436
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed
• [Journal Article] Identification of an S-RNase binding protein1 (SBP1) homolog of apple (<i>Malus</i>&times;<i>domestica</i>) (2013)
  • Author(s): Minamikawa, F. M., Fujii, D., Kakui, H., Kotoda, N. and Sassa, H.
  • Journal Title: Plant Biotechnology Volume: 30 Issue: 2 Pages: 119-123
  • DOI: 10.5511/plantbiotechnology.13.0109a
  • NAID: 10031184115
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Divergence of expression pattern contributed to neofunctionalization of duplicated HD-Zip l transcription factor in barley (2013)
  • Author(s): Shun Sakuma, Mohammad Pourkheirandish, Goetz Hensel, Jochen Kumlehn, Nils Stein, Akemi Tagiri, Naoki Yamaji, Jian Feng Ma, Hidenori Sassa, Takato Koba, Takao Komatsuda
  • Journal Title: New Phytologist Volume: 197 Issue: 3 Pages: 939-948
  • DOI: 10.1111/nph.12068
  • Peer Reviewed
• [Journal Article] Interhaplotypic heterogeneity and heterochromatic features may contribute to recombination suppression at the S locus in apple (Malus×domestica) (2012)
  • Author(s): Wang, S., Kakui, H., Kikuchi, S., Koba, T. and Sassa, H.
  • Journal Title: Journal of Experimental Botany Volume: 63 Issue: 2 Pages: 4983-4990
  • DOI: 10.1093/jxb/err313
  • Peer Reviewed
• [Journal Article] Polyacrylamide gel electrophoresis of S-RNase fragments for identification of S-genotypes of Japanese pear (Pyrus pyrifolia) (2012)
  • Author(s): Kato, M., Kato, S. and Sassa, H.
  • Journal Title: Breeding Science Volume: 62 Pages: 348-351
  • NAID: 10031131504
  • Peer Reviewed
• [Journal Article] Structural features of the S locus of apple. (2012)
  • Author(s): Sassa H., Wang, S., Minamikawa, M., Kakui, H., Kikuchi, S. and Koba, T.
  • Journal Title: ActaHorticulturae Volume: 967 Pages: 89-94
  • Peer Reviewed
• [Journal Article] Identification of self- incompatibility related proteins in the pistil of Japanese pear (Pyrus pyrifolia (Burm. f.)) by proteome analysis. (2012)
  • Author(s): Uchida, A., Sassa, H., Takenaka, S., Sakakibara, Y., Suiko, M. and Kunitake, H.
  • Journal Title: Plant Omics Journal Volume: 5 Pages: 320-325
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of expressed proteins in the different developmental stages of the style development of self-incompatible 'Hyuganatsu' (Citrus tamurana hort. ex Tanaka). (2012)
  • Author(s): Uchida, A., Takenaka, S., Sakakibara, Y., Kurogi, S., Honsho, C., Sassa, H., Suiko, M. and Kunitake, H.
  • Journal Title: Journal of the Japanese Society for Horticultural Science Volume: 81 Pages: 150-158
  • Peer Reviewed
• [Presentation] トマト野生種Solanum arcanum におけるSLF(Slocus F-box) の単離と花粉側自家和合性変異体の解 析 (2017)
  • Author(s): 茅野 史香, 小糸 恵, 毛利 勇太, 南川 舞, 近藤 勝彦, 神山 康夫, 佐々 英徳
  • Organizer: 日本育種学会
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-03-30
• [Presentation] 新規SFBB の単離と自家不和合性の弱いニホンナ シ品種‘なつひかり’の解析 (2017)
  • Author(s): 齋藤 直美, 佐々 英徳
  • Organizer: 日本育種学会
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-03-30
• [Presentation] 花粉液体培養系における定量的評価法の確立 (2017)
  • Author(s): 小川 洋一, 佐々 英徳
  • Organizer: 日本育種学会
  • Place of Presentation: 名古屋大学（愛知県、名古屋市）
  • Year and Date: 2017-03-30
• [Presentation] Identification and characterization of S-RNase binding protein 1(SBP1)homolog of apple (Malus×domesitica) (2012)
  • Author(s): Minamikawa, M., Fujii, D., Koyano, R., Kakui, H., Kotoda, N. and Sassa, H.
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: ホテル名古屋ガーデンパレス
  • Year and Date: 2012-11-15
• [Presentation] Characterization of S-RNase binding protein1 (SBP1) homolog of apple (Malus × donmestica) (2012)
  • Author(s): Minamikawa, M., Fuii, D., Koyano, R., Kakui, H., Kotoda, N. and Sassa, H.
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: ICC, Jeju, Korea
  • Year and Date: 2012-10-24
• [Presentation] リンゴにおけるCullinlホモログとMdSBP1(Malus×domestica S-Rnase binding protein1)のタンパク質間相互作用解析 (2012)
  • Author(s): 南川舞、小谷野瑠璃子、藤井大輔、佐々英徳
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-09-15
• [Presentation] リンゴS遺伝子座のハプロタイプ間配列多様性とヘテロクロマチン化 (2012)
  • Author(s): 王三紅、角井宏行、菊池真司、木庭卓人、佐々英徳
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-09-15
• [Presentation] バラ科果樹の自家不和合性における自己認識と非自己認識
  • Author(s): 佐々英徳, 南川舞, 角井宏行
  • Organizer: 日本育種学会第124回講演会
  • Place of Presentation: 鹿児島大学
  • Invited
• [Presentation] リンゴ花粉側S 因子候補SFBB とSSK1 (Skp1-like1) およびSBP1 (S-RNase binding protein1) のタンパ ク質間相互作用解析
  • Author(s): 南川 舞, 佐々 英徳
  • Organizer: 日本育種学会第124回講演会
  • Place of Presentation: 鹿児島大学
• [Presentation] 弱い自家不和合性を示すニホンナシ品種' なつひかり' における花粉S 遺伝子候補SFBBの解析
  • Author(s): 平岡 崇志, 南川 舞, 加藤 雅樹, 佐々 英徳
  • Organizer: 日本育種学会第124回講演会
  • Place of Presentation: 鹿児島大学