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Multi-omics study for understanding of secondary metabolite accumulation in grape berry and its application

Research Project

Project/Area Number 24380019
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionNagoya University

Principal Investigator

SHIRATAKE Katsuhiro  名古屋大学, 生命農学研究科, 准教授 (90303586)

Research Collaborator NAKABAYASHI Ryo  
FUKAO Yoichiro  
MARTINOIA Enrico  
JASINSKI Michal  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsブドウ / 二次代謝 / アントシアニン / レスベラトロール / トランスクリプトミクス / プロテオミクス / メタボロミクス / 代謝マップ / 二次代謝産物 / オミクス解析 / アントシアン / 果皮 / 培養細胞
Outline of Final Research Achievements

Grape accumulates several secondary metabolites, such as catechin, resveratrol and anthocyanin. In this study we profiled inductions of different secondary metabolite accumulations by different stimuli in grape berry skins or in grape culture cells using multi-omics, i.e. combination of transcriptomics, proteomics and metabolomics. This study revealed clear inductions of anthocyanin synthetic pathway in grape skin during berry ripening and in grape cells by light irradiation, and resveratrol synthetic pathway in grape skins by ultra violet irradiation and in grape cells by elicitor+jasmonic acid treatment.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (31 results)

All 2015 2014 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 4 results) Presentation (21 results) (of which Invited: 4 results)

  • [Journal Article] Multi omics in grape berry skin revealed specific induction of stilbene synthetic pathway by UV-C irradiation.2015

    • Author(s)
      Suzuki M., Nakabayashi R., Ogata Y., Sakurai N., Tokimatsu T., Goto S., Suzuki M., Jasinski M., Martinoia E., Otagaki S., Matsumoto S., Saito K. and Shiratake K.
    • Journal Title

      Plant Physiology

      Volume: - Issue: 1 Pages: 47-59

    • DOI

      10.1104/pp.114.254375

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular cloning and characterization of ABCG/PDR-type ABC transporter in grape berry skin.2014

    • Author(s)
      Suzuki M., Jasinski M., Martinoia M., Nakabayashi R., Suzuki M. and Shiratake K.
    • Journal Title

      Advances in Horticultural Science

      Volume: 28 Pages: 53-63

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Comprehensive element analysis of fruit flesh from European pear ‘La France’ and its giant fruit bud mutant indicates specific roles for B and Ca in fruit development.2014

    • Author(s)
      Reuscher S., Isuzugawa K., Kawachi M., Oikawa A. and Shiratake K.
    • Journal Title

      Scientia Horticulturae

      Volume: 176 Pages: 255-260

    • DOI

      10.1016/j.scienta.2014.07.019

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Transcriptome analysis of flower receptacles of the European pear (Pyrus communis L.) 'La France' and its giant fruit sport using next-generation sequencing technology.2014

    • Author(s)
      Nashima K., Terakami S., Nishitani C., Yamamoto T., Habu T., Takahashi H., Nakazono M., Isuzugawa K., Hanada T., Takashina T., Matsumoto S., Otagaki S., Mori H., Oikawa A. and Shiratake K.
    • Journal Title

      J. Hortic. Sci. Biotech.

      Volume: 89 Pages: 293-300

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular cloning and expression analyses of FaFT, FaTFL and FaAP1 genes in cultivated strawberry: their correlation to flower bud formation2014

    • Author(s)
      Nakajima R., Otagaki S., Yamada K., Shiratake K. and Matsumoto S.
    • Journal Title

      Biologia Plantarum

      Volume: -

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome analysis an European pear (Pyrus communis L.) and its giant fruit sport using next-generation sequencing technolog2014

    • Author(s)
      Nashima K., Terakami S., Nishitani C., Yamamoto T., Habu T., Takahashi H., Nakazono M., Isuzugawa K., Hanada T., Takashina T., Matsumoto S., Otagaki S., Mori H., Oikawa A. and Shiratake K.
    • Journal Title

      J. Hortic. Sci. Biotech.

      Volume: -

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microarray analysis of gene expression patterns during fruit development in European pear (Pyrus communis)2013

    • Author(s)
      Nashima K., Shimizu T., Nishitani C., Yamamoto T., Takahashi H., Nakazono M., Itai A., Isuzugawa K., Hanada T., Takashina T., Matsumoto S., Otagaki S., Oikawa A. and Shiratake K.
    • Journal Title

      Scientia Horticulturae

      Volume: 164 Pages: 466-473

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome analysis of giant pear fruit with fruit-specific DNA reduplication on a mutant branch2013

    • Author(s)
      Nashima K., Takahashi H., Nakazono M., Shimizu T., Nishitani C., Yamamoto T., Itai A., Isuzugawa K., Hanada T., Takashina T., Kato M., Matsumoto S., Oikawa A. and Shiratake K.
    • Journal Title

      J. Japan. Soc. Hort. Sci.

      Volume: 82 Pages: 301-311

    • NAID

      130004510794

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rose phytoene desaturase gene silencing by apple latent spherical virus vectors2013

    • Author(s)
      Ito H., Ochiai, M., Kato, H., Shiratake, K., Takemoto, D., Otagaki, S. and Matsumoto S.
    • Journal Title

      HortScience

      Volume: 47 Pages: 1278-1282

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 果樹における遺伝子の機能解明No.8 : 大果枝変わり酉洋ナシの統合オミクス解析2012

    • Author(s)
      及川彰, 白武勝裕
    • Journal Title

      果実日本

      Volume: 67 Pages: 107-111

    • Related Report
      2012 Annual Research Report
  • [Presentation] Genetic relationships among some persimmon rootstocks and their influence on scion tree growth2015

    • Author(s)
      Hu M.J., Zhang P.X., Chen L., Luo Z.R., Zhang Q.L. and Shiratake K.
    • Organizer
      平成27年度園芸学会春季大会
    • Place of Presentation
      千葉大学
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Update of the KEGG database and the KaPPA-View 4 KEGG system of grape and multi-omics study of grape berry skin2015

    • Author(s)
      Katsuhiro Shiratake, Mami Suzukia Ryo Nakabayashib Yoshiyuki Ogatac Nozomu Sakuraid Toshiaki Tokimatsu, Susumu Goto and Kazuki Saito
    • Organizer
      2nd Plant Genomics Congress Asia
    • Place of Presentation
      Kuala Lumpur
    • Year and Date
      2015-03-19 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] プロテオミクスによるセイヨウナシ果実成長における代謝のプロファイルと糖蓄積に関わるトランスポーターの同定2014

    • Author(s)
      ロイシャ シュテファン,森本玲奈,深尾陽一朗,白武勝裕
    • Organizer
      平成26年度園芸学会秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] タイプ2赤果肉リンゴ品種・系統ごとの着色関連遺伝子群の発現パターン2014

    • Author(s)
      佐藤秀人,太田垣駿吾,阿部和幸,岡田和馬,小松宏光,前島勤,白武勝裕,松本省吾
    • Organizer
      平成26年度園芸学会秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 異なる二次代謝産物蓄積様式を示すブドウ培養細胞の性状解析2014

    • Author(s)
      戸田奈津実,松本省吾,白武勝裕,太田垣 駿吾
    • Organizer
      平成26年度園芸学会秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] プロテオミクスによるブドウの新規アントシアニントランスポーターの探索2014

    • Author(s)
      阪本浩嗣, 深尾陽一朗郎, 太田垣駿吾, 松本省吾, 白武勝裕
    • Organizer
      第32回日本植物細胞分子生物学会大会・シンポジウム
    • Place of Presentation
      岩手大学
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] トマトのゲノムデータベースおよびESTデータベースにおけるABCトランスポーターの探索と発現解析果実の成長と品質に関わるトランスポーターの探索 -トマトにおけるゲノムワイド解析およびセイヨウナシとブドウにおけるマルチオミクス-2014

    • Author(s)
      白武勝裕,阪本浩嗣,川崎智世,Stefan Reuscher,秋山真仁,水野彩夏,鈴木真実,牧野治子,森本玲奈,太田垣駿吾
    • Organizer
      第9回トランスポーター研究会年会
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2014-06-14 – 2014-06-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] プロテオミクスによるブドウのを用いた新規アントシアニントランスポーターの探索2014

    • Author(s)
      阪本浩嗣, 深尾陽一朗郎, 太田垣駿吾, 松本省吾, 白武勝裕
    • Organizer
      第9回トランスポーター研究会年会
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2014-06-14 – 2014-06-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Multi-Omics of Bud Mutant of Pear Setting Giant Fruits2013

    • Author(s)
      K Shiratake, K Isuzugawa, A Oikawa, T Takashina, H Mori, R Nakabayashi, Y Jikumaru, T Hanada, K Nashima, R Morimoto, T Otsuka, M Suzuki, M Kato, S Otagaki, Y Fukao, Y Ogata, H Murayama
    • Organizer
      Gordon Research Conferences “Plant Metabolic Engineering”
    • Place of Presentation
      Boston
    • Related Report
      2013 Annual Research Report
  • [Presentation] Multi-omics of pear fruit and grape berry2013

    • Author(s)
      Katsuhiro Shiratake, Kanji Isuzugawa, Akira Oikawa et al.
    • Organizer
      7th EPSO Conference “Plants for a Greening Economy”
    • Place of Presentation
      Porto Heli
    • Related Report
      2013 Annual Research Report
  • [Presentation] Multi-omics for fruit developmental study2013

    • Author(s)
      Katsuhiro Shiratake
    • Organizer
      2013 International Symposium on Biodesign
    • Place of Presentation
      Busan
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Genome-wide identification and expression analysis ofABC transporters in Solanum lycopersicum.2013

    • Author(s)
      Mizuno, A., Sone, K., Suzuki, M., Hioki, J., Otagaki, S., Matsumoto, S., Aoki, K., Shibata, D. and Shiratake, K.
    • Organizer
      10th JSOL International Symposium on Solanaceae Genomics
    • Place of Presentation
      Osaka
    • Related Report
      2013 Annual Research Report
  • [Presentation] ブドウ果皮および培養細胞におけるアントシアニンおよびレスベラトロールの誘導とマルチオミクス解析2013

    • Author(s)
      阪本浩嗣, 鈴木真実, 中林亮,鈴木実,尾形善之,櫻井望,太田垣駿吾,松本省吾,白武勝裕
    • Organizer
      第31回日本植物細胞分子生物学会大会・シンポジウム
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] トマトのゲノムデータベースおよびESTデータベースにおけるABCトランスポーターの探索と発現解析2013

    • Author(s)
      水野彩夏,鈴木真美,曽根一友,日置淳平,太田垣駿吾,松本省吾,青木考,柴田大輔,白武勝裕
    • Organizer
      第31回日本植物細胞分子生物学会大会・シンポジウム
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] セイヨウナシおよびブドウにおける果実形質解析のためのマルチオミクス解析2013

    • Author(s)
      白武勝裕
    • Organizer
      平成25年度園芸学会秋季大会シンポジウム
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] トマトにおけるABCトランスポーターのゲノムワイド解析2013

    • Author(s)
      水野彩夏,鈴木真実,曽根一友,日置淳平,太田垣駿吾,松本省吾,青木考,柴田大輔,白武勝裕
    • Organizer
      平成25年度園芸学会秋季大会
    • Place of Presentation
      岩手
    • Related Report
      2013 Annual Research Report
  • [Presentation] 光刺激またはエリシター処理によるブドウ懸濁培養細胞の二次代謝誘導2013

    • Author(s)
      阪本浩嗣, 太田垣駿吾,松本省吾,白武勝裕
    • Organizer
      平成25年度園芸学会秋季大会
    • Place of Presentation
      岩手
    • Related Report
      2013 Annual Research Report
  • [Presentation] バラ科果樹統合オミクスデータベース“Fruits Omics Database”2013

    • Author(s)
      大塚貴生,中林亮,軸丸裕介,五十鈴川寛司,村山秀樹,斉藤和季,白武勝裕,及川彰
    • Organizer
      平成25年度園芸学会秋季大会
    • Place of Presentation
      岩手
    • Related Report
      2013 Annual Research Report
  • [Presentation] 実用化植物でのGO解析・パスウェイ解析用データベースの整備2013

    • Author(s)
      尾形善之, 櫻井望, 鈴木秀幸, 白武勝裕, 柴田大輔
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ブドウ懸濁培養細胞の増殖特性および光刺激による二次代謝産物蓄積の誘導2012

    • Author(s)
      阪本浩嗣, 太田垣駿吾, 松本省吾, 白武勝裕
    • Organizer
      園芸学会春季大会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] セイヨウナシのマルチオミクスとFruit Omics Database

    • Author(s)
      白武勝裕,大塚貴生,及川彰
    • Organizer
      平成26年度園芸学会春季大会小集会
    • Place of Presentation
      筑波
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2012-04-24   Modified: 2019-07-29  

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