Metal accumulation mechanism of Araliaceae plants and their application for phytoremediation of radioactive Sr.

• Project/Area Number: 24380038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Mie University
• Principal Investigator: MIZUNO Takafumi 三重大学, 生物資源学研究科, 准教授 (50346003)
• Co-Investigator(Kenkyū-buntansha): TAKENAKA Chisato 名古屋大学, 大学院生命農学研究科, 教授 (40240808) ; HARADA Emiko 滋賀県立大学, 環境科学部, 准教授 (20232845) ; TOMIOKA Rie 名古屋大学, 大学院生命農学研究科, 助教 (40456588)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
• Keywords: コシアブラ / 土壌浄化 / 放射性ストロンチウム / ヤナギ / タカノツメ / 放射性セシウム / ストロンチウム / セシウム / 金属集積 / ウコギ科植物 / 培養細胞 / 放射光解析

Outline of Final Research Achievements

Non-radioactive Sr concentration in Chengiopanax sciadophylloides in field showed a correlation with those of Mn and Ca. With SEM－EDX observation we could find crystal compounds that contain Ca and Sr in the bark of Salix sp, Chengiopanax sciadophylloides and Eleutherococcus innovan, suggesting that Sr absorbed in trees stored in crystalized materials and stored for long period. We also identified several genes including Ca-conjugated protein as genes related for Sr accumulation in trees. Roots with higher 137Cs had higher colonization rate of AM fungi in the Chengiopanax sciadophylloides rhizosphere.

Research Products

• [Journal Article] Elemental composition of plants from the serpentine soil of Sugashima Island, Japan (2015)
  • Author(s): Takafumi Mizuno and Yoko Kirihata
  • Journal Title: Australian Journal of Botany Volume: 未定 Issue: 4 Pages: 252-252
  • DOI: 10.1071/bt14226
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Induction of Nickel Accumulation in Response to Zinc Deficiency in Arabidopsis thaliana (2015)
  • Author(s): Sho Nishida , Aki Kato , Chisato Tsuzuki, Junko Yoshida and Takafumi Mizuno
  • Journal Title: International Journal of Molecular Science Volume: 16 Issue: 5 Pages: 9420-9430
  • DOI: 10.3390/ijms16059420
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Microbeam methodologies as powerful tools in manganese hyperaccumulation research: present status and future directions. (2013)
  • Author(s): Denise Rita Fernando, Alan Marshall, Alan John Martin Baker, Takafumi Mizuno.
  • Journal Title: Frontiers in Plant Physiology Volume: 4 Pages: 1-9
  • DOI: 10.3389/fpls.2013.00319
  • Peer Reviewed
• [Journal Article] Manganese hyperaccumulation from no-contaminated soil in Chengiopanax sciadophylloides and its correlation with calcium accumulation. (2013)
  • Author(s): Takafumi Mizuno, Kanae Emori and Shin-ichiro Ito
  • Journal Title: Soil Science and Plant Nutrition Volume: 59 Issue: 4 Pages: 591-602
  • DOI: 10.1080/00380768.2013.807213
  • Peer Reviewed
• [Presentation] 菅島の蛇紋岩土壌に生育する植物の元素組成と、ツゲの特異的なニッケル・鉄集積 (2015)
  • Author(s): 水野隆文
  • Organizer: 第126回日本森林学会大会
  • Place of Presentation: 北海道大学 札幌
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 木本植物におけるストロンチウム（Sr）蓄積機構 (2014)
  • Author(s): 原田英美子1，稲田和幸1，森卓弥1，木村ひろみ1，庄美冴子1，竹中千里2、富岡利恵2、保倉明子3、寺田靖子4、〇水野隆文5
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学生田キャンパス 神奈川県川崎市
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Distribution of arbuscular mycorrhizas within Chengiopanax sciadophylloides rootsystem contaminated with radiocesium (2014)
  • Author(s): Seitaro Deguchi, Yuki Sugiura, Chisato Takenaka, Yosuke Matsuda. Takafumi Mizuno, Hajime Ozawa,
  • Organizer: nternational symposium on physiological processes in roots of woody plants
  • Place of Presentation: Nagoya Univ. Japan.
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] Chemical characteristics of the plants grown on serpentine soil of Suga Island (2014)
  • Author(s): Yoko Kirihata and Takafumi Mizuno
  • Organizer: 8th International Conference on Serpentine Ecology
  • Place of Presentation: Kota Kinabalu, Malaysia
  • Year and Date: 2014-06-09 – 2014-06-13
• [Presentation] Manganese hyperaccumulation from non-contaminated soil in Chengiopanax sciadophylloides and its correlation with calcium accumulation. (2014)
  • Author(s): Takafumi Mizuno, Kanae Emori, Rie Tomioka, Emiko Harada, Chisato Takenaka and Shin-ichiro Ito
  • Organizer: 8th International Conference on Serpentine Ecology
  • Place of Presentation: Kota Kinabalu, Malaysia
  • Year and Date: 2014-06-09 – 2014-06-13
• [Presentation] Mineral properties of the plants grown on serpentine soil areas in Aichi and Wakayama prefectures (2014)
  • Author(s): Taiki Hori, Mai Matsushima, Takafumi Mizuno
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学/富山
• [Presentation] Function analysis of the N-terminal transcated form of NjZNT1, derived from a splicing variants in the Zn hyperaccumulator Noccaea japonicum. (2014)
  • Author(s): Sho Nishida, Sayaka Aisu, Takafumi Mizuno, Toru Fujiwara
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学/富山
• [Presentation] 放射光Ｘ線で読み解く植物の重金属蓄積機構 (2014)
  • Author(s): 原田英美子、保倉明子
  • Organizer: 第74回分析化学討論会
  • Place of Presentation: 日本大学、郡山市
  • Invited
• [Presentation] Each metal distribution and chemical form of Cd and Zn on the metal accumulator Gamblea innovans. (2014)
  • Author(s): 櫻井美鈴、富岡利恵、保倉明子、増山文博、寺田靖子
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学/富山
• [Presentation] Mineral properties of the plants grown on serpentine soil of Suga Island (2014)
  • Author(s): Mizuno et al （詳細未定）
  • Organizer: International Conference Serpentine Ecology
  • Place of Presentation: Kota Kinabalu/Malasia
• [Presentation] Manganese hyperaccumulation from no-contaminated soil in Chengiopanax sciadophylloides and its correlation with calcium accumulation. (2014)
  • Author(s): Mizuno et al （詳細未定）
  • Organizer: International Conference Serpentine Ecology
  • Place of Presentation: Kota Kinabalu/Malasia
• [Presentation] Characterization of Nickel and Zinc Tolerance, Accumulation and Mineral Profile in the Metal Hyperaccumulator Noccaea japonica (Noccaea cochleariforme) (2013)
  • Author(s): Sho Nishida,Junko Yoshida, Toshihiro Watanabe, Toru-Fujiwara, Takafumi Mizuno
  • Organizer: 17th International Plant Nutrition Colloquium (IPNC)
  • Place of Presentation: Istanbul / Turkey
• [Presentation] 東海・近畿地方の蛇紋岩土壌地帯における土壌の化学組成と植生、およびツゲのニッケル超集積について (2013)
  • Author(s): 水野隆文・堀 太紀
  • Organizer: 日本土壌肥料学会2013大会
  • Place of Presentation: 名古屋大学・名古屋
• [Presentation] Possible involvement of alternative splicing of TjZNTl in zinc accumulation in the metal hyperaccumulator Thlaspi japonicum (2013)
  • Author(s): Sho Nishida Ayaka Aisu Natsuki Nishimura, Takafumi Mizuno, Torn Fujiwara
  • Organizer: The 16th International Workshop on Plant Membrane Biology
  • Place of Presentation: Kurashiki (Japan)
• [Presentation] 鳥羽市菅島の蛇紋岩質土壌に分布する植物の金属集積特性 (2012)
  • Author(s): 水野隆文・桐畑陽子
  • Organizer: 日本土壌肥料学会2012大会
  • Place of Presentation: 鳥取大学(鳥取)
• [Presentation] 和歌山県天然記念物キイシモツケの分子系統分類学的および生理形態的性格付け : イワシモツケおよびトサシモツケとの比較 (2012)
  • Author(s): 明渡絵里朱, 平田智子, 上井和幸, 高木裕子, 水野隆文、小林真, 小池孝良, 大和勝幸, 秋田求,泉井桂
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学(姫路)
• [Presentation] タカネグンバイ由来ZIPトランスポーターTjZNTlの発現が酵母のニッケル耐性と亜鉛集積性にあたえる影響 (2012)
  • Author(s): 愛須彩加・西田翔・水野隆文
  • Organizer: 日本土壌肥料学会2012大会
  • Place of Presentation: 鳥取大学(鳥取)
• [Book] バイオベース元素戦略―都市鉱山・海底鉱山に眠る貴金属・レアメタル回収技術― (2015)
  • Author(s): 水野隆文その他
  • Publisher: シーエムシー
• [Remarks]
  • URL: http://www.bio.mie-u.ac.jp/junkan/busshitsu/lab4/SSPN/index.html