Analysis of coenzyme Q biosynthesis in eukaryotes

• Project/Area Number: 24380056
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Shimane University
• Principal Investigator: KAWAMUKAI MAKOTO 島根大学, 生物資源科学部, 教授 (70186138)
• Co-Investigator(Kenkyū-buntansha): KAINO Tomohiro 島根大学, 生物資源科学部, 准教授 (90541706)
• Co-Investigator(Renkei-kenkyūsha): MATSUO Yasuhiro 島根大学, 生物資源科学部, 助教 (70596832)
• Research Collaborator: YANAI Ryota ; MOCHIZUKI Shiomi ; YOKOMI Kazumasa
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: コエンザイムQ / ユビキノン / 分裂酵母 / 抗酸化物質 / coenzyme Q / ubiquinone / fission yeast / S. pombe / CoQ / PKA / 電子伝達系 / ミトコンドリア / 酵母 / イソプレノイド / 抗酸化物資 / 抗酸化 / 遺伝病 / 生合成 / システイン

Outline of Final Research Achievements

Coenzyme Q is essential for energy production through the electron transfer system and also acts as antioxidant. This study aims to understand the biosynthetic pathway of coenzyme Q in fission yeast. This analysis also contributes an understanding of human disease involved in CoQ synthesis. When we expressed genes for CoQ biosynthesis from human or plant into fission yeast, they complemented the function of fission yeast corresponding deletion mutants. The analysis of intermediate compounds accumulated in coq mutant was succeeded. The binding site of CoQ10 protein was unveiled in this study.

Research Products

• [Journal Article] Biosynthesis of coenzyme Q in eukaryotes. (2016)
  • Author(s): Kawamukai,M
  • Journal Title: Biosci Biotechnol Biochem. Volume: 80 Issue: 1 Pages: 23-33
  • DOI: 10.1080/09168451.2015.1065172
  • NAID: 120006819045
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Production of CoQ10 in fission yeast by expression of genes responsible for CoQ10 biosyntheis. (2015)
  • Author(s): Daisuke Moriyama, Kouji Hosono, Makoto Fujii, Motohisa Washida, Hirokazu Nanba, Tomohiro Kaino, Makoto Kawamukai
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: on line Issue: 6 Pages: 1-8
  • DOI: 10.1080/09168451.2015.1006573
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functional conservation of coenzyme Q biosynthetic genes among yeasts, plants, and humans. (2014)
  • Author(s): Kazuhiro Hayashi, Yuki Ogiyama, Kazumasa Yokomi, Tsuyoshi Nakagawa, Tomohiro Kaino, Makoto Kawamukai
  • Journal Title: PLoS ONE Volume: 9 Issue: 6 Pages: 1-14
  • DOI: 10.1371/journal.pone.0099038
  • NAID: 120007002193
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of the Binding Site of the Quinone-Head Group in Mitochondrial Coq10 by Photoaffinity Labeling (2014)
  • Author(s): Murai M, Matsunobu K, Kudo S, Ifuku K, Kawamukai M, Miyoshi H.
  • Journal Title: Biochemistry Volume: 53 Issue: 24 Pages: 3359-4003
  • DOI: 10.1021/bi500347s
  • Peer Reviewed
• [Journal Article] The effect of coenzyme Q10 included by γ-cyclodextrin on the growth of fission yeast studied by microscope Raman spectroscopy. (2013)
  • Author(s): Tatsuro Nishida, Tomohiro Kaino, Ryo Ikarashi, Daisuke Nakata, Keiji Terao, Masahiro Ando, Hiro-o Hamaguchi, Makoto Kawamukai, and Tatsuyuki Yamamoto
  • Journal Title: Journal of Molecular Structure Volume: 1048 Pages: 375-381
  • DOI: 10.1016/j.molstruc.2013.05.066
  • Peer Reviewed
• [Journal Article] The zinc finger protein Gsf1 regulates Gsf2-dependent flocculation in fission yeast. (2013)
  • Author(s): Matsuzawa, et al.
  • Journal Title: FEMS Yeast Research Volume: 13 Issue: 3 Pages: 259-266
  • DOI: 10.1111/1567-1364.12029
  • Peer Reviewed
• [Journal Article] Polypeptone Induces Dramatic Cell Lysis in ura4 Deletion Mutants of Fission Yeast. (2013)
  • Author(s): Yuzy Matsuo, Kouhei Nishino, Kouhei Mizuno, Takashi Akihiro, Takashi Todm, Yasuhiro Matsuo, Kaino and Makoto Kawamukai.
  • Journal Title: PLoS One Volume: 8 Issue: 3 Pages: 13-13
  • DOI: 10.1371/journal.pone.0059887
  • NAID: 120007002149
  • Peer Reviewed
• [Presentation] コエンザイムQ10の生合成と微生物生産 (2016)
  • Author(s): 川向誠, 戒能智宏
  • Organizer: 日本農芸化学会
  • Place of Presentation: 札幌（札幌コンベンションセンター）
  • Year and Date: 2016-03-29
  • Invited
• [Presentation] Biosynthesis of CoQ10 in fission yeast (2015)
  • Author(s): Makoto Kawamukai
  • Organizer: The 8th international CoQ10 association
  • Place of Presentation: Bologna, Italy (Centro San Domenico))
  • Year and Date: 2015-10-09
  • Int'l Joint Research / Invited
• [Presentation] 酵母の表現型に影響する培地成分の違い (2015)
  • Author(s): 川向 誠
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-29
  • Invited
• [Presentation] 分裂酵母CoQ欠損株が蓄積する中間体の解析 (2015)
  • Author(s): ○柳井 良太、赤井 華子、西野 耕平、戒能 智宏、川向 誠
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-27
• [Presentation] コエンザイムQ生合成遺伝子の酵母、ヒト、植物での保存性 (2014)
  • Author(s): 林 和弘、 戒能智宏、○川向 誠
  • Organizer: 日本生化学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] ゲノムを活用したCoQ10代謝工学 (2014)
  • Author(s): 川向 誠
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京（明治大学）
  • Invited
• [Presentation] ユビキノン結合性タンパク質Coq10におけるキノン結合部位の同定 (2014)
  • Author(s): 松延 広平 村井 正俊 川向 誠 三芳 秀人
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京（明治大学）
• [Presentation] 分裂酵母でのコエンザイムQ10の高生産 (2014)
  • Author(s): 森山 大輔 戒能 智宏 鷲田 元久 難波 弘憲 川向 誠
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京（明治大学）
• [Presentation] コエンザイムQの生合成と多機能性 (2013)
  • Author(s): 川向誠
  • Organizer: 日本農芸化学会 シンポジウム
  • Place of Presentation: 仙台(東北大学)(招待講演)
  • Year and Date: 2013-03-01
• [Presentation] Biosynthesis and various roles of coenzyme Q in yeasts (2012)
  • Author(s): Makoto Kawamukai
  • Organizer: The 7th conference of the international coenzyme Q10 associqtion
  • Place of Presentation: Seville, Spain(招待講演)
  • Year and Date: 2012-11-10
• [Book] コエンザイムQ１０の基礎と応用 (2015)
  • Author(s): 戒能智宏、川向誠
  • Total Pages: 256
  • Publisher: 丸善プラネット
• [Book] コエンザイムQ１０基礎と応用 (2015)
  • Author(s): 戒能智宏、川向誠
  • Total Pages: 200
  • Publisher: 丸善
• [Book] 酵母の生命科学と生物工学 (2013)
  • Author(s): 川向誠 戒能智宏 太田明徳 福田良一
  • Total Pages: 25
  • Publisher: 化学同人