| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Outline of Final Research Achievements |
Genetic inactivation of egg nucleus by UV-irradiation is prerequisite to induce androgenesis (all-male inheritance). We found that cold-shock (3°C for 30 min) of loach eggs just after fertilization eliminated egg nucleus together with second polar body release and sperm nucleus (male pronucleus) initiated androgenesis. Cold-shock of eggs fertilized with diploid sperm of tetraploid loach produced viable androgenetic diploid progeny. Heat-shock (42°C for 2 min) 35 min after the cold-shock treatment successfully produced androgenetic doubled haploids with homozygous genotypes in all the loci. In zebrafish, cold-shock (7°C for 30 min) of eggs just after fertilization also induced androgenetic haploids. Subsequent heat-shock of androgenetic haploids at the first cleavage successfully induced doubled haploids, which produced genetically identical spermatozoa. Cloning was succeeded by the second round production of androgenetic doubled haploids.
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