Development and application of induced androgenesis without irradiation of eggs
Project/Area Number |
24380100
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
General fisheries
|
Research Institution | Hokkaido University |
Principal Investigator |
ARAI KATSUTOSHI 北海道大学, 水産科学研究科(研究院), 教授 (00137902)
|
Co-Investigator(Kenkyū-buntansha) |
YAMAHA Etsuro 北海道大学, 北方生物圏フィールド科学センター, 教授 (60191376)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
|
Keywords | 倍加半数体 / 倍数体 / 配偶子 / クローン / 受精 / 染色体操作 / ドジョウ / ゼブラフィッシュ / 半数体 / 三倍体 / フローサイトメトリー / キンギョ / 純系 / マイクロサテライト / AFLP / 雄性発生二倍体 / 四倍体 / 卵核 |
Outline of Final Research Achievements |
Genetic inactivation of egg nucleus by UV-irradiation is prerequisite to induce androgenesis (all-male inheritance). We found that cold-shock (3°C for 30 min) of loach eggs just after fertilization eliminated egg nucleus together with second polar body release and sperm nucleus (male pronucleus) initiated androgenesis. Cold-shock of eggs fertilized with diploid sperm of tetraploid loach produced viable androgenetic diploid progeny. Heat-shock (42°C for 2 min) 35 min after the cold-shock treatment successfully produced androgenetic doubled haploids with homozygous genotypes in all the loci. In zebrafish, cold-shock (7°C for 30 min) of eggs just after fertilization also induced androgenetic haploids. Subsequent heat-shock of androgenetic haploids at the first cleavage successfully induced doubled haploids, which produced genetically identical spermatozoa. Cloning was succeeded by the second round production of androgenetic doubled haploids.
|
Report
(4 results)
Research Products
(5 results)