Application of novel labeling method for membrane proteins to aggregational analysis and its sophistication
| Project/Area Number |
24390009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YANO Yoshiaki 京都大学, 大学院薬学研究科, 助教 (60402799)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
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| Keywords | 膜タンパク質 / オリゴマー化 / 蛍光イメージング / クロスリンク / イメージング |
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| Outline of Final Research Achievements |
Membrane proteins include many important proteins for drug targets. Development of better experimental methods for membrane proteins is particularly important because of limitations in the current methods. Here we developed two in-cell analysis methods based on the coiled-coil labeling method. 1) An image analysis method for measuring protein oligomeric state was established and applied for various membrane proteins. 2) A crosslinking method was developed, which was useful for direct comparison of protein oligomeric states in live cells and solubilized micelles.
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Report
(4 results)
Research Products
(15 results)
