Elucidation of herpesvirus entry mechanism identifying its host receptor

• Project/Area Number: 24390114
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Kobe University
• Principal Investigator: MORI Yasuko 神戸大学, 医学(系)研究科(研究院), 教授 (50343257)
• Co-Investigator(Kenkyū-buntansha): TANG Huamin 神戸大学, 大学院医学研究科, 助教 (10595896)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: HHV-6 / ウイルス感染 / エントリー / 受容体 / HHV-6B / 侵入 / ウイルス / 感染症

Outline of Final Research Achievements

Human herpesvirus 6B (HHV-6B) causes exanthem subitum in little children, and then establishes lifelong latency in the host. It can reactivate from latency in immunocompromised patients and usually causes severe diseases. No therapeutic or prophylactic methods for HHV-6B infection are available. In the current study, we focused on the entry process of HHV-6B, and identified the cellular receptor which had been unidentified. Furthermore, we did detailed analysis of the interaction between the novel pair of receptor and viral ligand, and found the key domains for their interaction. Our achievement would contribute to the elucidation of the molecular mechanism of HHV-6B infection.

Research Products

• [Journal Article] Maturation of Human Herpesvirus 6A Glycoprotein O Requires Coexpression of Glycoprotein H and Glycoprotein L. (2015)
  • Author(s): Tang H, Mahmoud NF, Mori Y.
  • Journal Title: J. Virology Volume: 89 Issue: 9 Pages: 5159-63
  • DOI: 10.1128/jvi.00140-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Detailed study of the interaction between human herpesvirus 6B glycoprotein complex and its cellular receptor, human CD134. (2014)
  • Author(s): Tang H, Wang J, Mahmoud NF, Mori Y.
  • Journal Title: J. Virology Volume: 88 Issue: 18 Pages: 10875-82
  • DOI: 10.1128/jvi.01447-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CD134 is a cellular receptor specific for human herpesvirus-6B entry (2013)
  • Author(s): Tang, H et al.
  • Journal Title: PNAS Volume: 110 Issue: 22 Pages: 9096-9099
  • DOI: 10.1073/pnas.1305187110
  • Peer Reviewed
• [Journal Article] Identification of the Human Herpesvirus 6A gQ1 Domain Essential for Its Functional Conformation. (2013)
  • Author(s): Maeki et al.
  • Journal Title: Journal of Virology Volume: 87 Issue: 12 Pages: 7054-63
  • DOI: 10.1128/jvi.00611-13
  • Peer Reviewed
• [Presentation] Identification of an entry receptor specific for HHV-6B infection (2013)
  • Author(s): Huamin Tang
  • Organizer: 38th International Herpesvirus Workshop
  • Place of Presentation: Grand Rapids, Michigan, USA
• [Presentation] ヒトヘルペスウイルス-6A/Bの宿主細胞への侵入機構に関する研究 (2013)
  • Author(s): 森 康子
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
  • Invited
• [Presentation] Functional analysis of human herpesvirus-6B entry receptor and its viral ligand (2013)
  • Author(s): Huamin Tang
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸