• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a novel vascular-targeting therapy through stem cell research

Research Project

Project/Area Number 24390197
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionKyoto University

Principal Investigator

YAMASHITA Jun  京都大学, iPS細胞研究所, 教授 (50335288)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords血管新生 / 多能性幹細胞 / 内皮細胞 / 分化 / 血管内皮増殖因子 / アンジオポイエチン / 幹細胞 / 血管形成 / マイクロRNA / シグナル / キナーゼ
Outline of Final Research Achievements

Purpose of this study is that development of novel therapies for ischemic heart disease or cancer through manipulating vascular formation using advanced pluripotent stem cell research.
We demonstrated that kappa-opioid receptor suppressed tumor angiogenesis through inhibiting expression of type II vascular endothelial growth factor receptor. In addition, we reported a novel function of angiopoietin-1 in coronary vein formation as a collaboration with Department of cardiovascular medicine, University of Tokyo.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (56 results)

All 2015 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 5 results) Presentation (41 results) (of which Invited: 8 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Single-step generation of gene knockout-rescue system in pluripotent stem cells by promoter insertion with CRISPR/Cas92014

    • Author(s)
      Matsunaga T, Yamashita JK.*
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 444 Pages: 158-163

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dual modulation of the mitochondrial permeability transition pore and redox signaling synergistically promotes cardiomyocyte differentiation from pluripotent stem cells.2014

    • Author(s)
      Cho SW, Park JS, Heo HJ, Park SW, Song S, Kim I, Han YM, Yamashita JK, Youm JB, Han J, Koh GY.
    • Journal Title

      J Am Heart Assoc

      Volume: 3(2) Issue: 2

    • DOI

      10.1161/jaha.113.000693

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart2014

    • Author(s)
      Arita Y, Nakaoka Y, Matsunaga T, Kidoya H, Yamamizu K, Arima Y, Kataoka-Hashimoto T, Ikeoka K, Yasui T, Masaki T, Yamamoto K, Higuchi K, Park JS, Shirai M, Nishiyama K, Yamagishi H, Otsu K, Kurihara H, Minami T, Yamauchi-Takihara K, Koh GY, Mochizuki N, Takakura N, Sakata Y, Yamashita JK, Komuro I.
    • Journal Title

      Nat Commun

      Volume: 5 Pages: 4552-4552

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration.2014

    • Author(s)
      Masumoto H, Ikuno T, Takeda M, Fukushima H, Marui A, Katayama S, Shimizu T, Ikeda T, Okano T, Sakata R, Yamashita JK.
    • Journal Title

      Sci Rep

      Volume: 4 Issue: 1 Pages: 6716-6716

    • DOI

      10.1038/srep06716

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single-step generation of gene knockout-rescue system in pluripotent stem cells by promoter insertion with CRISPR/Cas9.2014

    • Author(s)
      Matsunaga T, Yamashita JK*
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 444 Issue: 2 Pages: 158-163

    • DOI

      10.1016/j.bbrc.2014.01.037

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kappa-Opioids inhibit tumor angiogenesis by suppressing VEGF signaling.2013

    • Author(s)
      Yamamizu K, Furuta S, Hamada Y, Yamashita A, Kuzumaki N, Narita M, Doi K, Katayama S, Nagase H, Yamashita JK, Narita M.
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 3213-3213

    • DOI

      10.1038/srep03213

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of k-opioid receptor agonist on the growth of non-small cell lung cancer (NSCLC) cells2012

    • Author(s)
      Kuzumaki N, Suzuki A, Narita M, Hosoya T, Nagasawa A, Imai S, Yamamizu K, Morita H, Nagase H, Okada Y, Okano HJ, Yamashita JK, Okano H, Suzuki T, Narita M
    • Journal Title

      Br J Cancer

      Volume: 106(Epub 2012 Feb 16) Issue: 6 Pages: 1148-1152

    • DOI

      10.1038/bjc.2011.574

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple analyses of G-protein coupled receptor (GPCR) expression in the development of gefitinib-resistance in transforming non-small-cell lung cancer.2012

    • Author(s)
      Kuzumaki N
    • Journal Title

      PLoS One

      Volume: 7 Issue: 10 Pages: e44368-e44368

    • DOI

      10.1371/journal.pone.0044368

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pluripotent stem cell-engineered cell sheets re-assembled with defined cardiovascular populations ameliorate reduction in infarct heart function with cardiomyocyte-mediated neovascularization2012

    • Author(s)
      Masumoto H, Matsuo T, Yamamizu K, Uosaki H, Narazaki G, Katayama S, Marui A, Shimizu T, Ikeda T, Okano T, Sakata R, Yamashita JK
    • Journal Title

      Stem Cells

      Volume: 30 Issue: 6 Pages: 1196-1205

    • DOI

      10.1002/stem.1089

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein Kinase A Determines Timing of Early Differentiation through Epigenetic Regulation with G9a2012

    • Author(s)
      Yamamizu K, Fujihara M, Tachibana M, Katayama S, Takahashi A, Hara E, Imai H, Shinkai Y, and Yamashita JK
    • Journal Title

      Cell Stem Cell

      Volume: 10 Issue: 6 Pages: 759-770

    • DOI

      10.1016/j.stem.2012.02.022

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PKA/CREB signaling triggers initiation of endothelial and hematopoietic cell differentiation via Etv2 induction2012

    • Author(s)
      Yamamizu K, Matsunaga T, Katayama S, Kataoka H, Takayama N, Eto K, Nishikawa SI, Yamashita JK
    • Journal Title

      Stem Cells

      Volume: 30 Issue: 4 Pages: 687-696

    • DOI

      10.1002/stem.1041

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 2型VEGF受容体陽性細胞純化再培養はヒトiPS細胞からの血管内皮細胞分化誘導を効率化する.2015

    • Author(s)
      幾野 毅
    • Organizer
      第18回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 2型VEGF受容体陽性細胞純化再培養によるヒトiPS細胞からの効率的血管内皮細胞分化誘導.2015

    • Author(s)
      幾野 毅
    • Organizer
      第45回日本心臓血管外科学会学術総会.
    • Place of Presentation
      京都
    • Year and Date
      2015-02-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Scalable and highly efficient endothelial cell differentiation methods from human induced pluripotent stem cells based on monolayer and serum-free culture for realization of regenerative medicine.2015

    • Author(s)
      幾野 毅
    • Organizer
      The 18th Takeda Science Foundation Symposium on Bioscience,
    • Place of Presentation
      大阪
    • Year and Date
      2015-01-15 – 2015-01-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 2型血管内皮細胞増殖因子受容体陽性細胞の純化再培養によるヒトiPS 細胞からの血管内皮細胞分化誘導の効率化2014

    • Author(s)
      幾野 毅
    • Organizer
      第67回日本胸部外科学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2014-10-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Scalable and highly efficient endothelial cell differentiation methods from human induced pluripotent stem cells for realization of regenerative medicine.2014

    • Author(s)
      幾野 毅
    • Organizer
      第5回Molecular Cardiovascular ConferenceⅡ.
    • Place of Presentation
      Kobe
    • Year and Date
      2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトiPS細胞由来心筋特異的前駆細胞の同定2014

    • Author(s)
      武田 匡史、山下 潤
    • Organizer
      第5回 Molecular Cardiovascular Conference Ⅱ
    • Place of Presentation
      神戸
    • Year and Date
      2014-09-05 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Scalable and highly efficient endothelial cell differentiation methods from human induced pluripotent stem cells based on monolayer and serum-free culture.2014

    • Author(s)
      Ikuno T.
    • Organizer
      ESC Congress2014.
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2014-08-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Scalable and highly efficient endothelial cell differentiation methods from human induced pluripotent stem cells based on monolayer and serum-free culture for realization of regenerative medicine.2014

    • Author(s)
      Ikuno T.
    • Organizer
      International Society for Stem Cell Research (ISSCR) 12th Annual Meeting.
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of novel chemicals potently inducing cardiomyocyte differentiation from mouse and human pluripotent stem cells2014

    • Author(s)
      Fukushima H.
    • Organizer
      ISSCR (International Society for Stem Cell Research) 12th Annual Meeting
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2014-06-18 – 2014-06-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification and application of human iPS derived-cardiac progenitor enriched population for cardiac regeneration.2014

    • Author(s)
      Takeda T, Yamashita JK.
    • Organizer
      The 3ird Retreat of Center for iPS Cell Research and Application (CiRA)
    • Place of Presentation
      Shiga
    • Year and Date
      2014-04-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Yamashita JK. Fate determination processes of endothelial lineages regulated by protein kinase A signaling in Pluripotent stem cell differentiation.2012

    • Author(s)
      K. Yamashita
    • Organizer
      Japan-Korea Joint Symposium of Vascular Biology.
    • Place of Presentation
      Tokushima, Japan.(招待講演)
    • Year and Date
      2012-12-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] RSK4 is a novel regulator of endothelial cells differentiation through direct inhibition of Protein kinase A activity.2012

    • Author(s)
      松永太一, 山下潤
    • Organizer
      第20回日本血管生物医学会学術大会.
    • Place of Presentation
      徳島
    • Year and Date
      2012-12-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] Differentiation Stage-specific Roles of Protein Kinase A Signaling in Endothelial Cell Lineages.2012

    • Author(s)
      K. Yamashita
    • Organizer
      2012 KAIST Mini-symposium. Stem cells and Vascular Biology.
    • Place of Presentation
      Daejeon. Korea.(招待講演)
    • Year and Date
      2012-10-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] 血管内皮分化におけるRSK4の役割.2012

    • Author(s)
      松永太一, 山下潤
    • Organizer
      第3回Molecular Cardiovascular Conference II.
    • Place of Presentation
      小樽
    • Year and Date
      2012-09-08
    • Related Report
      2012 Annual Research Report
  • [Presentation] RSK4 is a Novel Negative Regulator of Endothelial Differentiation via Direct Suppression of Protein Kinase A Activity (poster). BCVS (Basic Cardiovascular Sciences)2012

    • Author(s)
      Matsunaga T, Jun K. Yamashita
    • Organizer
      2012 Scientific Sessions, AHA (American Heart Association).
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2012-07-23
    • Related Report
      2012 Annual Research Report
  • [Presentation] RSK4 is a novel negative regulator of endothelial differentiation via direct suppression of cAMP/PKA signaling.2012

    • Author(s)
      松永太一, 山下潤
    • Organizer
      第10回メタボリックシンドロームカンファレンス
    • Place of Presentation
      京都
    • Year and Date
      2012-07-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] RSK4 regulates endothelial differentiation via inhibition of cAMP/PKA-mediated Flk1 induction.2012

    • Author(s)
      松永太一, 山下潤
    • Organizer
      第33回日本炎症・再生医学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-07-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] RSK4 regulates endothelial differentiation through suppression of cAMP/PKA-mediated Flk1 induction (poster)2012

    • Author(s)
      Matsunaga T, Jun K. Yamashita
    • Organizer
      17th International Vascular Biology Meeting.
    • Place of Presentation
      Wiesbaden, Germany.
    • Year and Date
      2012-06-04
    • Related Report
      2012 Annual Research Report
  • [Presentation] Diverse roles of protein kinase A signaling in early stage endothelial cell differentiation (Oral presentation).2012

    • Author(s)
      山下潤
    • Organizer
      17th International Vascular Biology Meeting.
    • Place of Presentation
      Wiesbaden, Germany.(招待講演)
    • Year and Date
      2012-06-04
    • Related Report
      2012 Annual Research Report
  • [Presentation] RSK4によるcAMP/PKAシグナルを介した新しい内皮細胞分化制御機構.2012

    • Author(s)
      松永太一, 山下潤
    • Organizer
      第85回日本内分泌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-04-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] cAMP/PKA signalingを介したRSK4による内皮細胞分化制御機構.2012

    • Author(s)
      松永太一, 山下潤
    • Organizer
      第49回日本臨床分子医学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2012-04-13
    • Related Report
      2012 Annual Research Report
  • [Presentation] RSK4 suppresses endothelial cell fate through direct inhibition of PKA activity. (poster)

    • Author(s)
      Matsunaga T.
    • Organizer
      ISSCR (International Society for Stem Cell Research) 11th Annual Meeting
    • Place of Presentation
      Boston, USA.
    • Related Report
      2013 Annual Research Report
  • [Presentation] 内皮細胞の分化制御機構におけるRSK4の役割

    • Author(s)
      松永 太一
    • Organizer
      第33回日本炎症・再生医学会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] RSK4による内皮細胞分化の新規抑制機構

    • Author(s)
      松永 太一
    • Organizer
      第4回Molecular Cardiovascular Conference II
    • Place of Presentation
      小樽
    • Related Report
      2013 Annual Research Report
  • [Presentation] 内皮細胞分化におけるRSK4の役割

    • Author(s)
      松永 太一
    • Organizer
      第21回日本血管生物医学会学術大会
    • Place of Presentation
      豊中
    • Related Report
      2013 Annual Research Report
  • [Presentation] Crispr/Cas9を用いたプロモーター配列挿入による簡便な誘導性遺伝子過剰発現系の構築とその幹細胞研究応用

    • Author(s)
      松永 太一
    • Organizer
      第13回日本再生医療学会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Efficient and Scalable Endothelial Cell Differentiation Method from Human Induced Pluripotent Stem Cells Based on 2-D Monolayer and Serum-Free Culture.

    • Author(s)
      Ikuno T, Masumoto H, Marui A, Ikeda T, Sakata R, Yamashita JK.
    • Organizer
      ISSCR(International Society for Stem Cell Research) 11th Annual Meeting
    • Place of Presentation
      Boston, USA.
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト人工多能性幹(iPS)細胞からの効率的な内皮細胞分化誘導法

    • Author(s)
      幾野 毅
    • Organizer
      第34回日本炎症再生医学会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Efficient endothelial cell differentiation protocol from human induced pluripotent stem cells based on monolayer and serum-free culture for realization of vascular regenerative medicine.Rapid Fire - Remodelling the vessel wall.

    • Author(s)
      Ikuno T, Masumoto H, Marui A, Ikeda T, Sakata R, Yamashita JK.
    • Organizer
      ESC Congress2013
    • Place of Presentation
      Amsterdam, Netherlands.
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト人工多能性幹(iPS)細胞からの効率的かつ高収量な内皮細胞分化誘導法

    • Author(s)
      幾野 毅
    • Organizer
      第4回Molecular Cardiovascular ConferenceII
    • Place of Presentation
      北海道
    • Related Report
      2013 Annual Research Report
  • [Presentation] 無血清二次元単層培養によるヒトiPS細胞からの高効率高収量な内皮細胞分化誘導法

    • Author(s)
      幾野 毅
    • Organizer
      第21回日本血管生物医学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒトiPS細胞を用いた二次元単層培養による効率的内皮細胞分化誘導法

    • Author(s)
      幾野 毅
    • Organizer
      第66回日本胸部外科学会定期学術集会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] ES細胞/iPS細胞を用いた心血管分化研究とその応用

    • Author(s)
      山下 潤
    • Organizer
      安全性評価研究会(特別講演)
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] ES細胞・iPS細胞を用いた心血管分化再生研究

    • Author(s)
      山下 潤
    • Organizer
      愛知Rising Force Meeting(特別講演)
    • Place of Presentation
      名古屋
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ES細胞/iPS細胞を用いた多面的心臓再生治療戦略の開発

    • Author(s)
      山下 潤
    • Organizer
      岐阜心不全研究会(特別講演)
    • Place of Presentation
      岐阜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Multiple and integrative approaches to cardiovascular diseases with stem cell technology.

    • Author(s)
      Yamashita JK.
    • Organizer
      International Conference on Endothelin 2013 (Special Guest Session)
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Vascular cell differentiation and tissue regeneration.

    • Author(s)
      Yamashita JK.
    • Organizer
      第86回日本生化学会国際シンポジウム「血管・リンパ管の発生・形成機構とシグナル伝達」
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 多能性幹細胞からの内皮細胞分化とその応用

    • Author(s)
      山下 潤
    • Organizer
      日本血管生物医学会シンポジウム
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ES細胞・iPS細胞を用いた多面的心臓再生治療戦略の開発

    • Author(s)
      山下 潤
    • Organizer
      奈良県立医科大学祭シンポジウム
    • Place of Presentation
      橿原
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 多能性幹細胞を用いた多面的心血管再生治療戦略の開発

    • Author(s)
      山下 潤
    • Organizer
      日本心血管内分泌代謝学会シンポジウム
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 多能性幹細胞を用いた心血管分化再生に関する多面的研究

    • Author(s)
      山下 潤
    • Organizer
      徳島脈管研究会(特別講演)
    • Place of Presentation
      徳島
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks] 山下研究室のホームページ

    • URL

      http://www.cira.kyoto-u.ac.jp/yamashita/achievement.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 山下研究室|京都大学iPS細胞研究所/再生医科学研究所

    • URL

      http://www.frontier.kyoto-u.ac.jp/es02/index.htm

    • Related Report
      2013 Annual Research Report
  • [Remarks] 山下研究室ホームページ

    • URL

      http://www.frontier.kyoto-u.ac.jp/es02/index.htm

    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 効率的な内皮細胞の誘導方法2013

    • Inventor(s)
      山下潤、幾野毅、松永太一
    • Industrial Property Rights Holder
      国立大学法人京都大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-116428
    • Filing Date
      2013-05-31
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi