| Project/Area Number |
24390250
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
MIYATA TOSHIYUKI 独立行政法人国立循環器病研究センター, 研究所, 部長 (90183970)
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| Co-Investigator(Kenkyū-buntansha) |
KOKAME Koichi 独立行政法人国立循環器病研究センター, 研究所, 室長 (40270730)
AKIYAMA Masashi 独立行政法人国立循環器病研究センター, 研究所, 室長 (30298179)
BANNO Fumiaki 独立行政法人国立循環器病研究センター, 研究所, 研究員 (00373514)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | 静脈血栓症 / 凝固制御因子 / ADAMTS13 / 血栓性血小板減少性紫斑病 / フォンビルブランド因子 / プロテインS / 血小板凝集 / 静脈血栓塞栓症 / 抗凝固機構 / 血栓症 / 遺伝子変異 / 血小板 |
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| Outline of Final Research Achievements |
We generated monoclonal antibodies to the protein S K196E mutant and developed a simple and reliable method for the identification of protein S K196E carriers. The novel method allowed identifying mutation carriers even under warfarin treatment or pregnancy. We found that ADAMTS13 deficiency promoted venous thrombus formation induced by the electrolytic stimulation in mouse model. We have performed the candidate gene analysis (C3, CFH, CFB, CFI, MCP, and THBD) in 46 patients with atypical hemolytic uremic syndrome, aHUS. We identified C3 I1157T mutation in multiple aHUS families who are mainly localized in the Kansai area.
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