Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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Outline of Final Research Achievements |
We identified many genes with higher expression in articular cartilage than in growth plate cartilage. Among them, Fgf18 was found to protect articular cartilage through the upregulation of Timp1, which has an inhibitory effect on matrix degrading enzymes. In addition, we established a series of experimental methods of sampling a minute region of articular cartilage from cryosections using laser microdissection (LMD), and then analyzing the gene expression profiles with a next generation sequencer. Using these means, we examined differences in the profiles among several layers which comprise articular cartilage.
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