Analysis of mechanisms underlying the differentiation of articular cartilage
| Project/Area Number |
24390348
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ONO Takashi 東京大学, 医学部附属病院, 届出診療医 (00506248)
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| Co-Investigator(Kenkyū-buntansha) |
OSHIMA Yasushi 東京大学, 医学部附属病院, 講師 (50570016)
INUI Hiroshi 東京大学, 医学部附属病院, 助教 (60583119)
中川 匠 帝京大学, 医学部附属病院, 教授 (90338385)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 関節軟骨 / 変形性関節症 / FGF18 / レーザーマイクロダイセクション / Fgf18 / Timp1 / マイクロアレイ / 再生医療 |
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| Outline of Final Research Achievements |
We identified many genes with higher expression in articular cartilage than in growth plate cartilage. Among them, Fgf18 was found to protect articular cartilage through the upregulation of Timp1, which has an inhibitory effect on matrix degrading enzymes.
In addition, we established a series of experimental methods of sampling a minute region of articular cartilage from cryosections using laser microdissection (LMD), and then analyzing the gene expression profiles with a next generation sequencer. Using these means, we examined differences in the profiles among several layers which comprise articular cartilage.
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Report
(4 results)
Research Products
(11 results)
