Study on inhibitory effects of synthetic Peptide derived from TRAF1 on osteoclast differentiation
| Project/Area Number |
24390437
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
MAKIHIRA Seicho 九州大学, 歯学研究科(研究院), 准教授 (80304450)
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| Co-Investigator(Kenkyū-buntansha) |
SHINOHARA Yoshinori 九州大学, 大学院歯学研究院, 助教 (00423533)
HATSUMI Nagadome 九州大学, 大学院歯学研究院, 助教 (30284516)
MASAKI Honda 日本大学, 歯学部, 准教授 (70361623)
峯 裕一 広島大学, 医歯薬保健学研究院, 特任助教 (60605989)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | TRAF1 / 骨吸収 / ペプチド / 破骨細胞 / 膜通過 / ＴＲＡＦ１ |
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| Outline of Final Research Achievements |
There is a need for the development of a material with few side effects to treat diseases of osteolysis, such as a bisphosphonate-related osteonecrosis of the jaw. We previously showed that TRAF1, a member of the TRAF family, is a negative regulator of RANKL-dependent osteoclastogenesis. We thus hypothesized that synthetic peptides derived from TRAF1 could have a similar function in pre-osteoclast cells. Two peptides derived from TRAF1 (T1 and T2), N-terminally conjugated with an eleven-arginine sequence (11R), were synthesized. 11R is well known as a membrane-permeable sequence.T1 decreased the number of TRAP-positive osteoclasts in RAW264.7 cells stimulated with RANKL compared to those in RANKL- stimulated cells without T1. On the other hand, 11R and T2 had no effect on these in RAW264.7 exposed to RAW264.7RANKL.The results suggest that a synthetic peptide derived from TRAF1 may be a candidate of therapeutic agent to block osteolysis.
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Report
(4 results)
Research Products
(24 results)
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[Presentation] Inhibition of Cellular Fusion during RANKL-Dependent Osteoclastogenesis by NHE10-specific Monoclonal Antibody2013
Author(s)
Yuichi Mine, Seicho Makihira , Hiroki Nikawa, Toshihisa Kawai T, Masaru Ohara, Kazuko Kawahara, Koji Ohta, Takahiro Shuto, Toshio Kukita, Yoshihiro Terada
Organizer
2nd Meeting of the International Association for Dental Research Asia Pacific Region
Place of Presentation
Bangkok, Thailand
Related Report
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