| Project/Area Number |
24390442
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MORITA Ikuo 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60100129)
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| Co-Investigator(Kenkyū-buntansha) |
KOMAKI Motohiro 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座准教授授 (30401368)
IWASKI Kengo 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座講師 (40401351)
ONODERA Mitsue 東京医科歯科大学, 大学院医歯学総合研究科, 講師 (50376703)
中浜 健一 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (60281515)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 再生医療 / 歯根膜 / 間葉系幹細胞 / 骨損傷 / エキソソーム / 歯周組織 / 組織再生 / 抗炎症 / ナノメディスン / 歯周病 / 印刷技術 |
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| Outline of Final Research Achievements |
After we established the rat alveolar bone defect model, we have examined the effect of both our developed transfer-grafting method and conditioned medium injection method using this animal model. As a result, periodontal ligament-derived mesenchymal stem cells transferring to the decellularized amniotic membrane cause to rapid tissue regeneration. Furthermore, to allow for allotransplantation, when a collagen sponge including the contained cultured medium of the periodontal ligament-derived mesenchymal stem cells was applied to the same model, similar periodontal tissue regeneration was observed. We determined the presence of VEGF and exosomes in the cultured medium. In vitro experiment, we observed that the exosomes can differentiate the fibroblasts to the mesenchymal stem cells. The observation present new mechanism that mesenchymal stem cells can repair the defected tissues.
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