| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
In this research, we have succeeded to establish the enrichment of the viral genomes from wildlife intestinal contents.
Thereafter, we performed a deep sequencing using isolated and enriched viral nucleic acids. We have finally detected a novel bufavirus named as Mpulungu bufavirus from shrew feces. We have determined the almost whole genome sequences of the novel bufavirus which has a homology with that of human bufavirus (more than 51-53% of amino acid identity).
Based on the obtained sequences, we established a nested PCR method for screening of bufaviruses in spleen samples from wildlife. Finally, we obtained the relatively high positive rate of detection of the viral genome in shrew (17/49=34.7%). Our established nested PCR method is useful for screening of the bufaviruses in mammals.
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