Mechanisms of insulin resistance after 24 hours physical inactivity in murine soleus muscle.
Project/Area Number |
24500867
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied health science
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Research Institution | Juntendo University |
Principal Investigator |
KAKEHI SAORI 順天堂大学, 医学(系)研究科(研究院), 特任助教 (00450560)
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Co-Investigator(Kenkyū-buntansha) |
SAKURABA Keisyoku 順天堂大学, スポーツ健康科学部, 教授 (50175460)
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Co-Investigator(Renkei-kenkyūsha) |
TAMURA Yoshifumi 順天堂大学, 医学部, 准教授 (80420834)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 骨格筋 / インスリン抵抗性 / 骨格筋細胞内脂質 / 不活動 / 細胞内脂質 |
Outline of Final Research Achievements |
Physical inactivity induce insulin resistance. To elucidate its mechanism, we performed hind-limb cast immobilization (HCI) for 24 hours in C57BL6J mice and investigated ex-vivo insulin-stimulated 2-deoxy glucose uptake, insulin signal transduction and lipid composition in soleus muscle. 24h HCI induced insulin resistance in skeletal muscle with impaired insulin signal. In parallel these chenges, 24h HCI dramatically increased intramyocellular diacylglycerol (IMDG). Associated with the IMDG accumulation, expression and activity of lipid metabolism enzymes, which induce diacylglycerol accumulation, was increased after 24h HCI. These data suggested that 24h HCI induce insulin resistance through IMDG accumulation in skeletal muscle. Given that increased lipid metabolism enzymes activity and expression might be involved in the mechanism of IMDG accumulation by 24h HCI.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Effects of sitagliptin on ectopic fat contents and glucose metabolism in type 2 diabetic patients with fatty liver: A pilot study2014
Author(s)
Watanabe T, Tamura Y, Kakehi S, Funayama T, Takeno K, Kawaguchi M, Yamamoto L, Sato F, Ikeda S, Taka H, Fujimura T, Fujitani Y, Kawamori R, Watada H
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Journal Title
J Diabetes Invest
Volume: Mar;6(2)
Issue: 2
Pages: 164-72
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Association between expression of FABPpm in skeletal muscle and insulin sensitivity in intramyocellular-lipid-accumulated non-obese men2014
Author(s)
Kawaguchi M, Tamura Y, Kakehi S, Takeno K, Sakurai Y, Watanabe T, Funayama T, Sato F, Ikeda S, Ogura Y, Saga N, Naito H, Fujitani Y, Kanazawa A, Kawamori R, Watada
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Journal Title
J Clin Endocrinol Metab
Volume: Sep;99(9)
Issue: 9
Pages: 3343-52
DOI
Related Report
Peer Reviewed
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[Journal Article] Exercise-induced enhancement of insulin sensitivity is associated with accumulation of M2-polarized macrophages in mouse skeletal muscle.2013
Author(s)
Ikeda S1, Tamura Y, Kakehi S, Takeno K, Kawaguchi M, Watanabe T, Sato F, Ogihara T, Kanazawa A, Fujitani Y, Kawamori R, Watada H.
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Journal Title
Biochem Biophys Res Commun.
Volume: 441
Pages: 36-41
Related Report
Peer Reviewed
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[Presentation] Accumulation of intramyocellular lipid after high-fat diet is associated with the expression of genes involved in lipid metabolism in non-obese men2014
Author(s)
Kakehi S, Tamura Y, TakenoK, Sakurai Y, Kawaguchi M, Watanabe T, Funayama T, Sato F, Ikeda S, Kanazawa A, Fujitani Y, Kawamori R, and Watada H
Organizer
3rd international conference on Recent Advances and Controversies in Measuring Energy Metabolism
Place of Presentation
Tokyo
Year and Date
2014-10-10 – 2014-10-12
Related Report
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[Presentation] Physical inactivity and high fat diet synergically induce insulin resistance in skeletal muscle through PTP1B activation.2012
Author(s)
Kakehi S, Tamura Y, Takeno K, Kawaguchi M, Watanabe T, Funayama T, Sato F, Ikeda S, Fujitani Y, Kawamori R,Watada H
Organizer
American Diabetes Association 72st scientific sessions
Place of Presentation
Philadelphia
Related Report
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[Presentation] Characteristics of non-obese japanese men with muscle and/or liver insulin resistance.2012
Author(s)
Takeno K, Tamura Y, Kawaguchi M, Watanabe T, Funayama T, Sakurai Y, Sato F, Yamamoto R, Kim M, Kakehi S, Ikeda S, Giacca A, Fujitani Y, Hirose T, Kawamori R,Watada H
Organizer
American Diabetes Association 72st scientific sessions
Place of Presentation
Philadelphia
Related Report
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[Presentation] シダグリプチンが異所性脂肪蓄積、糖代謝に与える効果2012
Author(s)
田村好史, 渡邉隆宏, 船山崇, 竹野景海, 川口美奈子, 筧佐織, 山本理紗子, 藤谷与士夫, 弘世貴久, 河盛隆造,綿田裕孝
Organizer
第55回日本糖尿病学会年次学術集会
Place of Presentation
神奈川
Related Report