A novel pathogenic and defence mechanisms in diabetic angiopathy
| Project/Area Number |
24500967
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Ibaraki Christian University (2013-2014)
Yamagata University (2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATO Hideyo 新潟大学, 医学部・保健学科, 教授 (60235380)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 糖尿病性血管障害 / 酸化ストレス / 血管内皮細胞 / 培養細胞 / グルタチオン / 糖尿病 / 血管障害 / 鉄 / 生理活性 / 生体分子 |
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| Outline of Final Research Achievements |
To investigate the novel mechanism involved in oxidative stress-mediated diabetic angiopathy, we focused on function of the glutathione (GSH) redox cycle in endothelial cells. In diabetes model human umbilical vein endothelial cells (HUVEC) of the living state, the H2O2 scavenging activity via the GSH cycle was significantly reduced. We have carried out the synthesis of the causative agent. Although the synthetic compound was initially unstable, it was possible to obtain a final stable compound at a high purity. We found that this compound inhibits the H2O2 scavenging enzyme. As a protective factor of diabetic angiopathy, we found that myricetin, a food ingredient, inhibits apoptosis in diabetes model HUVEC. These substances may, at least in part, be directly involved in a novel mechanism leading from hyperglycemia to develop vascular injury.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] System xc- is a mediator of microglial function and its deletion slows symptoms in Amyotrophic Lateral Sclerosis.2015
Author(s)
Mesci, P., Zaïdi, S., Lobsiger, C. S., Millecamps, S., Escartin, C., Seilhean, D., Sato, H., Mallat, M., and Boillée, S
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Journal Title
Brain
Volume: 138
Issue: 1
Pages: 53-68
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Absence of system xc- in mice decreases anxiety and depressive-like behavior without affecting sensorimotor function or spatial vision.2015
Author(s)
Bentea, E., Demuyser, T., Liefferinge, J. V., Albertini, G., Deneyer, L., Nys, J., Merckx, E., Michotte, Y., Sato, H., Arckens, L., Massie, A., and Smolders, I.
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Journal Title
Progress in Neuro-Psychopharmacology & Biological Psychiatry
Volume: 59
Pages: 49-58
Related Report
Peer Reviewed
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[Journal Article] Cystathionine is a novel substrate of cystine/glutamate transporter: implications for immune function2015
Author(s)
Kobayashi, S., Sato, M., Kasakoshi, T., Tsutsui, T., Sugimoto, M., Osaki, M., Okada, F., Igarashi, K., Hiratake, J., Homma, T., Conrad, M., Fujii, J., Soga, T., Bannai, S., Sato, H.
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Journal Title
J. Biol. Chem.
Volume: 290
Issue: 14
Pages: 8778-8788
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Nrf2- and ATF4-dependent up-regulation of xCT modulates the sensitivity of T24 bladder carcinoma cells to proteasome inhibition.2014
Author(s)
Peng Ye, P., Mimura J., Okada, T., Sato, H., Liu, T., Maruyama, A., Ohyama, C., and Itoh, K.
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Journal Title
Mol. Cell. Biol.
Volume: 34
Issue: 18
Pages: 3421-3434
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mutation of ATF4 mediates resistance of neuronal cell lines against oxidative stress by inducing xCT expression.2012
Author(s)
Lewerenz, J., Sato, H., Albrecht, P., Henke, N., Noack, R., Methner, A., and Maher, P.
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Journal Title
Cell Death & Differentiation
Volume: 19
Issue: 5
Pages: 847-858
DOI
Related Report
Peer Reviewed
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[Presentation] Cystathionine is a physiological substrate of cystine/glutamate transporter, System xc-.2013
Author(s)
Kobayashi, S., Kasakoshi, T., Tsutsui, T., Azumi, S., Okada, T., Soga, T., Igarashi, K., Bannai, B., and Sato, H.
Organizer
37th Congress of the International Union of Physiological Sciences
Place of Presentation
Birmingham, U. K.
Related Report
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[Presentation] Role of cystine/glutamate transporter, System xc-, on NO release from macrophages.
Author(s)
Kobayashi, S., Sambe, Y., Tsutsui, T., Bannai, S., and Sato, H.
Organizer
16th Society for Free Radical Research International Meeting
Place of Presentation
London（イギリス）
Related Report
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