| Project/Area Number |
24501317
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Kagoshima University |
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Principal Investigator |
KUBOTA Naoko 鹿児島大学, 医歯(薬)学総合研究科, 助教 (40569810)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Masahiro 鹿児島大学, 医用ミニブタ先端開発研究センター, 教授 (30287099)
NOGUCHI Hirofumi 琉球大学, 医学部, 教授 (50378733)
SAITOH Issei 新潟大学, 医歯学総合研究科, 准教授 (90404540)
INADA Emi 鹿児島大学病院, 教授 (30448568)
SAITOH Yoko 新潟大学, 医歯学総合研究科, 助教 (30404487)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 癌幹細胞 / Oct-3/4 / 遺伝子工学的処理 |
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| Outline of Final Research Achievements |
We consider that Oct-3/4, a member of the family of POU-domain transcriptional factors, and alkaline phosphatase (ALP) can be the promising markers for cancer stem cells (CSCs). Herein, we examined expression of Oct-3/4 and ALP using 6 established human pancreatic carcinoma cell lines. RT-PCR analysis revealed expression of these two mRNAs in those cells. Immunocytochemical and cytochemical staining revealed that these proteins are mosaically present in PANC-1 cell line, one of those 6 cell lines (23% and 19%, respectively). However, Oct-3/4-positive PANC-1 cells did not exhibit overt ATP-binding cassette transporter G2 (ABCG2) activity, as revealed by Hoechst 33342 dye exclusion assay. Transfection of PANC-1 cells with an Oct-3/4 promoter-directed, enhanced green fluorescent protein (EGFP) construct confirmed the presence of Oct-3/4-positive cells. These findings indicate that in PANC-1 cells there are at least 2 subset populations, namely Oct-3/4-positive and ALP-positive cells.
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