Oct-3/4, a member of the family of POU-domain transcriptional factors can be the promising markers for cancer stem cells.
| Project/Area Number |
24501317
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Kagoshima University |
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Principal Investigator |
KUBOTA Naoko 鹿児島大学, 医歯(薬)学総合研究科, 助教 (40569810)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Masahiro 鹿児島大学, 医用ミニブタ先端開発研究センター, 教授 (30287099)
NOGUCHI Hirofumi 琉球大学, 医学部, 教授 (50378733)
SAITOH Issei 新潟大学, 医歯学総合研究科, 准教授 (90404540)
INADA Emi 鹿児島大学病院, 教授 (30448568)
SAITOH Yoko 新潟大学, 医歯学総合研究科, 助教 (30404487)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 癌幹細胞 / Oct-3/4 / 遺伝子工学的処理 |
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| Outline of Final Research Achievements |
We consider that Oct-3/4, a member of the family of POU-domain transcriptional factors, and alkaline phosphatase (ALP) can be the promising markers for cancer stem cells (CSCs). Herein, we examined expression of Oct-3/4 and ALP using 6 established human pancreatic carcinoma cell lines. RT-PCR analysis revealed expression of these two mRNAs in those cells. Immunocytochemical and cytochemical staining revealed that these proteins are mosaically present in PANC-1 cell line, one of those 6 cell lines (23% and 19%, respectively). However, Oct-3/4-positive PANC-1 cells did not exhibit overt ATP-binding cassette transporter G2 (ABCG2) activity, as revealed by Hoechst 33342 dye exclusion assay. Transfection of PANC-1 cells with an Oct-3/4 promoter-directed, enhanced green fluorescent protein (EGFP) construct confirmed the presence of Oct-3/4-positive cells. These findings indicate that in PANC-1 cells there are at least 2 subset populations, namely Oct-3/4-positive and ALP-positive cells.
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Report
(4 results)
Research Products
(13 results)
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[Presentation] piggyBac-transposon-mediated gene-delivery efficiently generates stable transfectants from HDDPCs and HDDPC-derived-iPSCs.2015
Author(s)
Soda M, Saitoh I, Inada E, Murakami T, Iwase Y, Kubota N, Sawami T, Matsumoto Y, Yamasaki Y, Hayasaki H, Ohshima H, Sato M
Organizer
IADR
Place of Presentation
Boston (America)
Year and Date
2015-03-11 – 2015-03-14
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[Presentation] ヒト乳歯歯髄由来iPS細胞樹立におけるフィーダー細胞選択の重要性2013
Author(s)
村上智哉, 齊藤一誠, 稲田絵美, 岩瀬陽子, 長谷川大子, 窪田直子, 松本祐子, 大島邦子, 岡 暁子, 山崎要一, 早崎治明
Organizer
第５１回日本小児歯科学会
Place of Presentation
岐阜
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[Presentation] 小児の口呼吸における関連因子の抽出と口唇閉鎖力との関連性についての先行研究2012
Author(s)
村上 大輔 稲田 絵美, 齊藤 一誠, 海原 康孝, 奥 猛志, 岩崎 智憲, 長谷川 大子, 深水 篤, 佐藤 秀夫, 武元 嘉彦, 窪田 直子, 伊藤 千晶, 乃村 俊樹, 田中 みゆき, 井形 紀子, 香西 克之, 山崎 要一
Organizer
第５０回日本小児歯科学会
Place of Presentation
東京
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