Studies on phosphorus limitation in microbial communities of forest soils

• Project/Area Number: 24510012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental dynamic analysis
• Research Institution: Shinshu University
• Principal Investigator: KUNITO Takashi 信州大学, 学術研究院理学系, 准教授 (90304659)
• Co-Investigator(Renkei-kenkyūsha): NAGAOKA Kazunari 独立行政法人農業, 食品産業技術総合研究機構・中央農業総合研究センター土壌肥料研究領域, 主任研究員 (80355544)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: リン / ホスファターゼ / 資源配分モデル / 土壌 / 土壌微生物 / リン制限 / 可給態リン / 黒ボク土 / 畑土壌

Outline of Final Research Achievements

It was found that microorganisms preferentially expended resources in the form of phosphatase production rather than β-D-glucosidase production to acquire phosphorus in soils with low phosphorus availability. Specific microbial species might predominate for production of phosphatase in low phosphorus available soils. The ratio of acid or alkaline phosphatase to β-D-glucosidase activities would be useful for evaluating phosphorus availability in soils.

Research Products

• [Journal Article] Assessment of phosphorus bioavailability in cultivated Andisols from a long-term fertilization field experiment using chemical extractions and soil enzyme activities. (2015)
  • Author(s): Moro, H., Kunito, T., and Sato, T.
  • Journal Title: Arch. Agron. Soil Sci. Volume: 61 Issue: 8 Pages: 1107-1123
  • DOI: 10.1080/03650340.2014.984697
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Soil microorganisms are less susceptible than crop plants to potassium deficiency. (2014)
  • Author(s): Moro, H., Kunito, T., Saito, T., Yaguchi, N., and Sato, T.
  • Journal Title: Arch. Agron. Soil Sci. Volume: 印刷中 Issue: 12 Pages: 1807-1813
  • DOI: 10.1080/03650340.2014.918960
  • Peer Reviewed
• [Journal Article] Phosphorus limitation in microorganisms leads to high phosphomonoesterase activity in acid forest soils. (2012)
  • Author(s): Kunito, T., Tobitani, T., Moro, H., and Toda, H.
  • Journal Title: Pedobiologia Volume: 55 Issue: 5 Pages: 263-270
  • DOI: 10.1016/j.pedobi.2012.05.002
  • Peer Reviewed
• [Presentation] 施肥管理の異なる畑土壌の酵素活性と酵素産生遺伝子を標的としたPCR-DGGEによる微生物群集構造解析 (2014)
  • Author(s): 諸 人誌、國頭 恭、齋藤龍司、矢口直輝、大塚重人、長岡一成
  • Organizer: 環境微生物系学会合同大会
  • Place of Presentation: アクトシティー浜松コングレスセンター(静岡県浜松市)
  • Year and Date: 2014-10-21 – 2014-10-24
• [Presentation] 施肥管理の異なる畑土壌におけるホスファターゼ活性とアルカリホスファターゼ生産細菌の群集構造解析 (2014)
  • Author(s): 諸 人誌、國頭 恭、齋藤龍司、矢口直輝、大塚重人、長岡一成
  • Organizer: 日本土壌肥料学会2014年度東京大会
  • Place of Presentation: 東京農工大学(東京都小金井市)
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] 畑土壌中の可給態窒素評価法の比較 (2014)
  • Author(s): 藤田一輝、國頭 恭、松下純子、中村 芳、諸 人誌、吉田清志
  • Organizer: 日本土壌肥料学会2014年度東京大会
  • Place of Presentation: 東京農工大学(東京都小金井市)
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] Evaluation of phosphorus bioavailability using soil enzyme activities: comparison with chemical extraction methods and crop P content. (2014)
  • Author(s): Moro, H., Kunito, T., and Sato, T.
  • Organizer: 20th World Congress of Soil Science
  • Place of Presentation: Jeju (Korea)
  • Year and Date: 2014-06-08 – 2014-06-13
• [Presentation] Phosphorus or nitrogen limitation in microorganisms in some Japanese forest soils. (2014)
  • Author(s): Moro, H., Kunito, T., Komukai, Y., and Toda, H.
  • Organizer: 20th World Congress of Soil Science
  • Place of Presentation: Jeju (Korea)
  • Year and Date: 2014-06-08 – 2014-06-13
• [Presentation] 酵素活性法を用いた畑土壌のリン利用性の評価 (2013)
  • Author(s): 諸 人誌、國頭 恭、佐藤 強
  • Organizer: 日本土壌微生物学会2013年度大会
  • Place of Presentation: 東京農工大学
• [Presentation] 黒ボク土におけるリン利用性評価法の検討―化学的抽出法と酵素活性法によるアプローチ― (2013)
  • Author(s): 諸 人誌、國頭 恭、佐藤 強
  • Organizer: 日本土壌肥料学会2013年度名古屋大会
  • Place of Presentation: 名古屋大学
• [Presentation] Assessment of phosphorus bioavailability in cultivated Andisols from the long-term field experiment using chemical extractions and soil enzyme activities. (2013)
  • Author(s): Moro, H., Kunito, T., and Sato, S.
  • Organizer: The 11th International Conference of the East and Southeast Asia Federation of Soil Science Societies (ESAFS)
  • Place of Presentation: Bogor, Indonesia
• [Presentation] 硝酸態窒素濃度の異なる2つの小渓流域での窒素動態 (2012)
  • Author(s): 戸田任重、小松仁美、内田祐未、國頭 恭
  • Organizer: 日本地球惑星科学連合2012年大会
  • Place of Presentation: 幕張メッセ国際会議場
• [Presentation] 大麦根圏中のリン形態と微生物群集の基質利用パターンの変動 (2012)
  • Author(s): 諸 人誌、國頭 恭、朴 虎東、佐藤 強
  • Organizer: 日本土壌微生物学会2012年度大会
  • Place of Presentation: 神戸大学
• [Presentation] 施肥管理の違いが土壌微生物群集の生理的状態へ与える影響 (2012)
  • Author(s): 諸 人誌、國頭 恭、朴 虎東、佐藤 強
  • Organizer: 日本土壌肥料学会2012年度鳥取大会
  • Place of Presentation: 鳥取大学