| Project/Area Number |
24510038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | Toho University |
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Principal Investigator |
OHNO Akira 東邦大学, 医学部, 講師 (40223903)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Naoyuki 東邦大学, 医学部, 准教授 (50104154)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | Legionella pneumophila / Serogroup1 / Subgroup / Sequence type / 臨床分離株 / 環境分離株 / 64kb pathogenic island / 塩素耐性 / L.pneumophila SG1 / 温泉水分離株 / 冷却塔水分離株 / LLhv typeIV A 分泌システム / Acanthamoeba / Serogroup 1 / Type IVA分泌システム / 65ｋｂ ｐａｔｈｏｇｅｎｉｃ ｉｓｌａｎｄ / Ａｃａｎｔｈａｍｏｅｂａ ｃａｓｔｅｌｌａｎｉｉ / ＭＬＳＴ / 温泉水 / 冷却塔水 / PCR based sequencing / MLST / Lvh typeIV SS / 65 kb pathogenic island / Acanthamoeba castellanii / Genome sequence / PCR based subgrouping |
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| Outline of Final Research Achievements |
This study demonstrated that the characteristics of Legionella pneumophila serogroup1(SG1), the major causative agent of Legionnaires' disease, was distinctly different between clinical isolates and environmental isolates despite of the same serogroup, and the environmental isolates carried frequently the strain-specific elements(the Lvh typeIVA secretion system and the 65kb pathogenic island(PI)) not shared by a number of clinical isolates. Further we found that the 65kb PI had a role for chlorine tolerance. Finally, we concluded that the fact that the Benidorm type subgroups, most common in clinical isolates, were not found in cooling tower isolates but relatively predominant in hot spring isolates might relate to that legionella infection occurs mostly at spa facilities.
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