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Development of toxicity test for algae by exposing recalcitrant dissolved organic matter accumulated in lakes

Research Project

Project/Area Number 24510044
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental impact assessment/Environmental policy
Research InstitutionLake Biwa Environmental Research Institute

Principal Investigator

HAYAKAWA Kazuhide  滋賀県琵琶湖環境科学研究センター, その他部局等, その他 (80291178)

Co-Investigator(Kenkyū-buntansha) SUGIYAMA Yuko  兵庫県立大, 環境人間学部, 准教授 (40305694)
TANAKA Hitoshi  埼玉県環境科学国際センター, 水土壌研究領域, 専門研究員 (40415378)
FUJITAKE Nobuhide  神戸大学, 農学研究科(研究院), 教授 (50243332)
MARUO Masahiro  滋賀県立大学, 環境科学部, 准教授 (80275156)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords影響評価手法 / 毒性評価 / 溶存有機物 / 難分解性 / 化学特性評価 / フルボ酸 / 琵琶湖 / 急性毒性 / COD / クラミドモナス / 蛍光スペクトル / フミン物質 / フミン酸
Outline of Final Research Achievements

Recalcitrant dissolved organic matter (DOM) is a possible factor of increasing COD in several closed-water areas in Japan, including Lake Biwa. In this study, we conducted to collect fulvic acid in Lake Biwa, and to clarify its chemical properties as a representative of recalcitrant DOM. Furthermore, we examined growth inhibition test of green alga Chlamydomonas, which is one of ecological impact test of chemical substances.
The results of the algae growth inhibition test using Chlamydomonas, the inhibition effect was not observed on the fulvic acid of Lake Biwa. We concluded that the fulvic acid under existing concentration present concentration in the lake was no inhibition effect for aquatic organisms, suggested by the present study and crustaceans and fish inhibition tests in another researches.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (38 results)

All 2015 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (31 results) (of which Invited: 1 results)

  • [Journal Article] Quantitative Monitoring of Aquatic Humic Substances in Lake Biwa, Japan, Using the DAX-8 Batch2015

    • Author(s)
      Morimaru Kida; Kenzo Maki; Akihiro Takata; Taku Kato; Kumiko Tsuda; Kazuhide Hayakawa; Yuko Sugiyama; Nobuhide Fujitake
    • Journal Title

      Organic Geochemistry

      Volume: 83-84 Pages: 153-157

    • DOI

      10.1016/j.orggeochem.2015.03.015

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 淡水における金属の化学スペシエーション:総合論文2015

    • Author(s)
      丸尾雅啓
    • Journal Title

      海洋化学研究

      Volume: 28 Pages: 2-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 琵琶湖における新たな水質指標に関する研究2014

    • Author(s)
      早川和秀・岡本高弘・五十嵐恵子・古角恵美・廣瀬佳則・一瀬諭・古田世子・田中稔・永田貴丸・津田久美子・清水芳久・日下部武敏・三崎健太郎・中野伸一・藤嶽暢英・山田悦・布施泰朗・田中仁志・杉山裕子・丸尾雅啓
    • Journal Title

      滋賀県琵琶湖環境科学研究センター 研究報告書

      Volume: 10 Pages: 52-75

    • Related Report
      2014 Annual Research Report
    • Open Access / Acknowledgement Compliant
  • [Journal Article] 有機汚濁と環境基準 -琵琶湖を例にして-2014

    • Author(s)
      津田久美子、早川和秀、岡本高弘
    • Journal Title

      用水と廃水

      Volume: 56(3) Pages: 59-72

    • NAID

      40020000011

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Investigations of environmental changes based on chemical analyses of humic substances.2013

    • Author(s)
      Kawahigashi, M., Ono, K., Iimura, Y. and Fujitake, N.
    • Journal Title

      Humic Substances Research

      Volume: 10 Pages: 1-9

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Seasonal characteristics of surface water fulvic acids from Lake Biwa and Lake Tankai in Japan2012

    • Author(s)
      Fujitake, N., Tsuda, K., Mori, H., Aso S., Kodama, H., Maruo, M., Yonebayashi, K.
    • Journal Title

      Limnology

      Volume: 13 Pages: 45-53

    • NAID

      10030761028

    • Related Report
      2012 Research-status Report
  • [Journal Article] Method for quantitative analysis of aquatic humic substances in clear water2012

    • Author(s)
      Tsuda, K., Takata, A., Shirai, H., Kozaki, K., Fujitake, N.
    • Journal Title

      Analytical Sciences

      Volume: 28 Pages: 1017-1020

    • Related Report
      2012 Research-status Report
  • [Presentation] 琵琶湖における新たな有機物管理と指標の検討2015

    • Author(s)
      早川和秀,岡本高弘,古角恵美,廣瀬佳則,田中稔, 一瀬諭,古田世子, 津田久美子
    • Organizer
      日本水環境学会第49回大会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 固相抽出法を用いた琵琶湖溶存有機物の分離と化学的特性の解析2015

    • Author(s)
      内海理伽、早川和秀、藤嶽暢英、高橋勝利、木田森丸、真木謙造、熊谷哲、杉山裕子
    • Organizer
      日本陸水学会近畿支部会
    • Place of Presentation
      京都大学
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 環境中の天然有機物の化学構造2014

    • Author(s)
      藤嶽暢英
    • Organizer
      日本腐植物質学会第30回講演会 記念シンポジウム
    • Place of Presentation
      日本大学藤沢キャンパス
    • Year and Date
      2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 琵琶湖及びその集水域におけるヒ素の化学種別分布2014

    • Author(s)
      長岡愛理、丸尾雅啓
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学
    • Year and Date
      2014-09-15 – 2014-09-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 好気的水中における溶存Fe(Ⅱ)の安定化に寄与する配位子の定量2014

    • Author(s)
      小山和香、小畑元、丸尾雅啓
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学
    • Year and Date
      2014-09-15 – 2014-09-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 活性アルミナを用いて分離した琵琶湖溶存有機物の化学的特徴2014

    • Author(s)
      内海理伽、早川和秀、藤嶽暢英、高橋勝利、木田森丸、真木謙造、 熊谷哲、杉山裕子
    • Organizer
      日本陸水学会 第79回大会
    • Place of Presentation
      筑波大学
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 琵琶湖における新たな有機物管理の考え方について2014

    • Author(s)
      早川和秀,岡本高弘,五十嵐恵子,古角恵美,廣瀬佳則,一瀬諭,田中稔
    • Organizer
      日本陸水学会 第79回大会
    • Place of Presentation
      筑波大学
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 琵琶湖および河川における溶存態ケイ素の形態について2014

    • Author(s)
      丸尾雅啓、辻衿奈
    • Organizer
      日本陸水学会 第79回大会
    • Place of Presentation
      筑波大学
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Distribution and chemical characteristics of dissolved organic matter in Lake Biwa extracted by hydrophobic adsorbents2014

    • Author(s)
      Rika Utsumi, Kazuhide Hayakawa, Nobuhide Fujitake, Katsutoshi Takahashi, Fumiko Kitano, Reiji Kojima, Miyako Hara, Akihiro Takata, Tetsu Kumagai, Yuko Sugiyama
    • Organizer
      The 16th International Symposium on River and Lake Environment
    • Place of Presentation
      韓国 春川市
    • Year and Date
      2014-08-25 – 2014-08-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Re-assessment of dissolved organic matter in Lake Biwa using UV absorption and fluorescence2014

    • Author(s)
      Kazuhide Hayakawa, Reiji Kojima, Chizuru Wada, Rika Utsumi, Yuko Sugiyama, Tetsu Kumagai, Nobuhide Fujitake
    • Organizer
      The 16th International Symposium on River and Lake Environment
    • Place of Presentation
      韓国 春川市 LADENA Resort
    • Year and Date
      2014-08-24 – 2014-08-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Distribution and chemical characteristics of dissolved organic matter in Lake Biwa extracted by activated alumina2014

    • Author(s)
      Rika Utsumi, Kazuhide Hayakawa, Nobuhide Fujitake, Katsutoshi Takahashi, Morimaru Kida, Kenzo Maki, Tetsu Kumagai, Yuko Sugiyama
    • Organizer
      The 16th International Symposium on River and Lake Environment
    • Place of Presentation
      韓国 春川市
    • Year and Date
      2014-08-24 – 2014-08-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Characterization of highly strong metal binding ligands in waters2014

    • Author(s)
      Maruo, M., Obata, H., Mitamura, O., Akatsuka, T., Amahashi, N., Azumi, H.,
    • Organizer
      The 16th International Symposium on River and Lake Environments
    • Place of Presentation
      韓国 春川市 LADENA Resort
    • Year and Date
      2014-08-24 – 2014-08-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Pseudopolarography による琵琶湖水中銅配位子の評価2014

    • Author(s)
      丸尾雅啓,小畑元
    • Organizer
      JpGU Meeting 2014
    • Place of Presentation
      横浜パシフィコ
    • Year and Date
      2014-04-28 – 2014-05-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] 琵琶湖水中のフルボ酸が藻類と甲殻類へ与えるについて2014

    • Author(s)
      早川和秀,廣瀬佳則,岡本高弘,一瀬諭,古田世子,田中仁志,三崎健太郎,日下部武敏,清水芳久,藤嶽暢英
    • Organizer
      日本水環境学会第48回年会
    • Place of Presentation
      東北大学川内北キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 単細胞緑藻クラミドモナスの鞭毛再生による湖沼蓄積性難分解性物質の影響2014

    • Author(s)
      田中仁志,早川和秀,藤嶽 暢英,中村省吾,西村修
    • Organizer
      日本水環境学会第48回年会
    • Place of Presentation
      東北大学川内北キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 国内外の湖沼や河川におけるフミン物質含量―DAX-8樹脂吸着法による―2014

    • Author(s)
      木田森丸・真木謙造・津田久美子・早川和秀・杉山裕子・川東正幸・大塚俊之・廣田充・加藤拓・藤嶽暢英
    • Organizer
      第25回日本陸水学会近畿支部講演会
    • Place of Presentation
      摂南大学寝屋川キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 固相抽出法を用いた琵琶湖溶存有機物の分布および化学的性質の検討2014

    • Author(s)
      内海理伽,早川和秀,藤嶽暢英,木田森丸,真木謙造,熊谷哲,杉山裕子
    • Organizer
      第25回日本陸水学会近畿支部講演会
    • Place of Presentation
      摂南大学寝屋川キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 琵琶湖水中における溶存有機物の光分解に与える金属の影響2014

    • Author(s)
      片岡寛大,丸尾雅啓
    • Organizer
      第25回日本陸水学会近畿支部講演会
    • Place of Presentation
      摂南大学寝屋川キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 琵琶湖水中のフルボ酸の生物影響評価手法について2013

    • Author(s)
      早川和秀,岡本高弘,一瀬諭,古田世子,田中仁志,三崎健太郎,日下部武敏,清水芳久,藤嶽暢英
    • Organizer
      日本陸水学会第78回大会
    • Place of Presentation
      龍谷大学瀬田校舎
    • Related Report
      2013 Research-status Report
  • [Presentation] 活性アルミナを用いた琵琶湖溶存有機物の分画法の検討2013

    • Author(s)
      内海理伽,早川和秀,藤嶽暢英,木田森丸,真木謙造,熊谷哲,杉山裕子
    • Organizer
      日本陸水学会第78回大会
    • Place of Presentation
      龍谷大学瀬田校舎
    • Related Report
      2013 Research-status Report
  • [Presentation] 琵琶湖水中のフルボ酸の生物影響評価について2013

    • Author(s)
      早川和秀,岡本高弘,一瀬諭,古田世子,田中仁志,三崎健太郎,日下部武敏,清水芳久,藤嶽暢英
    • Organizer
      日本腐植物質学会第29回講演会
    • Place of Presentation
      佐賀大学本庄キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 湖沼や河川水の腐植物質含有量はどのくらいを占めるか2013

    • Author(s)
      木田森丸,真木謙造,津田久美子,早川和秀,杉山裕子,川東正幸,大塚俊之,加藤拓,藤嶽暢英
    • Organizer
      日本腐植物質学会第29回講演会
    • Place of Presentation
      佐賀大学本庄キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 湖沼に蓄積する難分解性溶存有機物の藻類に及ぼす影響2013

    • Author(s)
      田中仁志、早川和秀、一瀬諭、藤嶽暢英、中村省吾:
    • Organizer
      第47回日本水環境学会年会
    • Place of Presentation
      大阪工業大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Long-term changes in the concentrations of nutrients and water quality parameters in Lake Biwa using integrated analysis from several monitoring data.2012

    • Author(s)
      K. Hayakawa, S. Tsujimura, T. Ishikawa, H. Haga, T. Okamoto, C. Jiao, K. Ishikawa, M. Kumagai
    • Organizer
      Association for Sciences of Limnology and Oceanography, Aquatic Sciences Meeting 2012
    • Place of Presentation
      びわ湖ホール
    • Related Report
      2012 Research-status Report
  • [Presentation] Development and application of a method for quantitative analysis of aquatic humic substances in clear water2012

    • Author(s)
      Tsuda, K., Takata, A., Sugiyama, Y., Hayakawa, K., Fujitake, N.
    • Organizer
      Association for Sciences of Limnology and Oceanography, Aquatic Sciences Meeting 2012
    • Place of Presentation
      びわ湖ホール
    • Related Report
      2012 Research-status Report
  • [Presentation] 琵琶湖水中の紫外吸収・蛍光物質の分布と特性の評価2012

    • Author(s)
      早川和秀,小島礼慈,和田千弦,杉山裕子,熊谷哲
    • Organizer
      2012年度日本陸水学会第77回大会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 琵琶湖水中の水系フミン物質の季節および深度別の変化2012

    • Author(s)
      木田森丸, 高田明弘, 津田久美子, 光嵜克敏, 白井秀門, 北野史子, 原都, 小島礼慈, 杉山裕子, 早川和秀, 藤嶽暢英
    • Organizer
      日本腐植物質学会第28回講演会
    • Place of Presentation
      首都大学東京
    • Related Report
      2012 Research-status Report
  • [Presentation] フミン物質の化学構造特性とその多様性2012

    • Author(s)
      藤嶽暢英
    • Organizer
      日本腐植物質学会第28回講演会 シンポジウム「腐植物質から環境を読む」
    • Place of Presentation
      首都大学東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Comparison of Dissolved Organic Matter (DOM) in Lake Biwa Separated by C18 and DAX Solid Phase Extraction

    • Author(s)
      Utsumi, R., Sugiyama, Y., Hayakawa, K., Kitano, F., Kojima, R., Hara, M., Takata, A., Fujitake, N.
    • Organizer
      Association for Sciences of Limnology and Oceanography, Aquatic Sciences Meeting 2012
    • Place of Presentation
      びわ湖ホール
    • Related Report
      2012 Research-status Report
  • [Presentation] C18, DAX固相抽出法によって分離された琵琶湖溶存有機物の分布と化学的特徴の比較

    • Author(s)
      内海 理伽, 早川 和秀,藤嶽 暢英,高田 明弘,小島 礼慈,北野 史子,熊谷 哲,杉山 裕子
    • Organizer
      2012年度日本陸水学会第77回大会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Research-status Report
  • [Presentation] C18、DAX固相抽出法による琵琶湖溶存有機物の分離と特徴の比較

    • Author(s)
      内海理伽,早川和秀,藤嶽暢英,高田明弘,小島礼慈,北野史子,熊谷哲,杉山裕子
    • Organizer
      陸水学会近畿支部会第24回研究発表会
    • Place of Presentation
      滋賀大学教育学部
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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