Elucidation of a novel feedback regulation in response to drought stress in plants

• Project/Area Number: 24510312
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Japan International Research Center for Agricultural Sciences
• Principal Investigator: FUJITA Yasunari 独立行政法人国際農林水産業研究センター, 生物資源・利用領域, 主任研究員 (00446395)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 脱リン酸化酵素 / フィードバック制御 / 植物 / アブシシン酸 / ストレス応答 / プロテインキナーゼ / 環境適応 / シロイヌナズナ / 乾燥ストレス / ABA / 浸透圧ストレス / 遺伝子発現 / 脱リン酸化

Outline of Final Research Achievements

Group A Protein Phosphatase 2Cs (PP2Cs) function as key components in ABA signaling in response to osmotic stresses by regulating AREB/ABF transcription factors via SnRK2 protein kinases in Arabidopsis plants. Here, we show that group Aa PP2Cs such as ABI1 are involved in fundamental functions in plant survival, whereas group Ab PP2Cs, including HAI1, play an important role especially under osmotic stress conditions.

Research Products

• [Journal Article] Four Arabidopsis AREB/ABF transcription factors function predominantly in gene expression downstream of SnRK2 kinases in abscisic-acid signaling in response to osmotic stress (2015)
  • Author(s): Takuya Yoshida, Yasunari Fujita, Kyonoshin Maruyama, Junro Mogami, Daisuke Todaka, Kazuo Shinozaki & Kazuko Yamaguchi-Shinozaki
  • Journal Title: Plant Cell and Enviorment Volume: 38 Issue: 1 Pages: 35-49
  • DOI: 10.1111/pce.12351
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Two Distinct Families of Protein Kinases Are Required for Plant Growth under High External Mg2+ Concentrations in Arabidopsis (2015)
  • Author(s): Junro Mogami, Yasunari Fujita, Takuya Yoshida, Yoshifumi Tsukiori, Hirofumi Nakagami, Yuko Nomura, Toru Fujiwara, Sho Nishida, Shuichi Yanagisawa, Tetsuya Ishida, Fuminori Takahashi, Kyoko Morimoto, Satoshi Kidokoro, Junya Mizoi, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
  • Journal Title: Plant Physiology Volume: 167 Issue: 3 Pages: 1039-1057
  • DOI: 10.1104/pp.114.249870
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of the Promoter Region of an Arabidopsis Gene for 9-cis-Epoxycarotenoid Dioxygenase Involved in Dehydration-Inducible Transcription. (2013)
  • Author(s): Behnam B, Iuchi S, Fujita M, Fujita Y, Takasaki H, Osakabe Y, Yamaguchi-Shinozaki K, Kobayashi M, Shinozaki K.
  • Journal Title: DNA Res. Volume: 20(4) Issue: 4 Pages: 315-24
  • DOI: 10.1093/dnares/dst012
  • Peer Reviewed
• [Journal Article] Pivotal role of the AREB/ABF-SnRK2 pathway in ABRE-mediated transcription in response to osmotic stress in plants. (2013)
  • Author(s): Fujita, Y., Yoshida, T., Yamaguchi-Shinozaki, K.
  • Journal Title: Physiologia Plantarum Volume: 147 Issue: 1 Pages: 15-27
  • DOI: 10.1111/j.1399-3054.2012.01635.x
  • Peer Reviewed
• [Journal Article] Overexpression of the ABA-dependent AREB1 transcription factor from Arabidopsis thaliana improves soybean tolerance to water-deficit. (2013)
  • Author(s): Barbosa, E.G.G., Leite, J.P., Marin, S.R.R., Marinho, J.P., Carvalho, J.F.C., Fuganti-Pagliarini, R., Farias, J.R.B., Neumaier, N., et al.
  • Journal Title: Plant Mol. Biol. Rep. Volume: 31 Issue: 3 Pages: 719-730
  • DOI: 10.1007/s11105-012-0541-4
  • Peer Reviewed
• [Journal Article] Arabidopsis AHP2, AHP3, and AHP5 histidine phosphotransfer proteins function as redundant negative regulators of drought stress response. (2013)
  • Author(s): Nishiyama R, Watanabe Y, Leyva-Gonzalez MA, Ha CV, Fujita Y, Tanaka M, Seki M, Yamaguchi-Shinozaki K, Shinozaki K, Herrera-Estrella L, Tran LS.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 110(12) Issue: 12 Pages: 4840-5
  • DOI: 10.1073/pnas.1302265110
  • Peer Reviewed
• [Journal Article] Natural variation in a polyamine transporter determines paraquat tolerance in Arabidopsis. (2012)
  • Author(s): Fujita, M., Fujita, Y., Iuchi, S., Yamada, K., Kobayashi, Y., Urano, K., Kobayashi, M., Yamaguchi-Shinozaki, K., Shinozaki, K.
  • Journal Title: Proc. Nat. Acad. Sci. USA Volume: 109 Issue: 16 Pages: 6343-6347
  • DOI: 10.1073/pnas.1121406109
  • Peer Reviewed
• [Journal Article] Purification, crystallization and preliminary X-ray analysis of OsAREB8 from rice, a member of the AREB/ABF family of bZIP transcription factors, in complex with its cognate DNA. (2012)
  • Author(s): Miyazono, K., Koura, T., Kubota, K., Yoshida, T., Fujita, Y., Yamaguchi-Shinozaki, K., Tanokura, M.
  • Journal Title: Acta Cryst. Sect. F Volume: 68 Issue: 4 Pages: 491-494
  • DOI: 10.1107/s1744309112009384
  • Peer Reviewed
• [Journal Article] Rice phytochrome-interacting factor-like protein OsPIL1 functions as a key regulator of internode elongation and induces a morphological response to drought stress. (2012)
  • Author(s): Todaka, D., Nakashima, K., Maruyama, K., Kidokoro, S., Osakabe, Y., Ito, Y., Matsukura, S., Fujita, Y., Yoshiwara, K., et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Issue: 39 Pages: 15947-15952
  • DOI: 10.1073/pnas.1207324109
  • Peer Reviewed
• [Presentation] シロイヌナズナにおいてSnRK2およびそれらの相互作用因子SDB1プロテインキナーゼはMg2+感受性を調節している (2015)
  • Author(s): 最上惇郎、藤田泰成、吉田拓也、月居佳史、中神弘史、野村有子、藤原徹、西田翔、柳澤修一、石田哲也、森本恭子、城所聡、溝井順哉、篠崎一雄、篠崎和子
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Four Arabidopsis AREB/ABF transcription factors predominantly regulate gene expression downstream of SnRK2 kinases in abscisic acid signaling. (2014)
  • Author(s): Yoshida, T., Fujita, Y., Maruyama, K., Mogami, J., Todaka, D., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 9th Tri National Arabidopsis Meeting 2014
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2014-09-24 – 2014-09-26
• [Presentation] Novel roles of subclass III SnRK2s and their interacting partners in modulating Mg2+-susceptibility in Arabidopsis (2014)
  • Author(s): Mogami, J., Fujita, Y., Yoshida, T., Tsukiori, Y., Nakagami, H., Nomura, Y., Fujiwara, T., Nishida, S., Yanagisawa, S., Ishida, T., Morimoto, K., Kidokoro, S., Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: Gordon Research Conference: Salt & Water Stress in Plants
  • Place of Presentation: Newry, USA
  • Year and Date: 2014-08-03 – 2014-08-08
• [Presentation] シロイヌナズナの乾燥ストレス応答において負の制御因子として働くヒスチジンリン酸基転移タンパク質AHP2, AHP3, AHP5 の機能解析 (2014)
  • Author(s): Nishiyama ,R.，Watanabe, Y.，Leyva-Gonzalez, M.，Ha, C.V.，Fujita, Y.，Tanaka, M.，Seki, M.，Yamaguchi-Shinozaki, K.，Shinozaki, K.，Herrera-Estrella, L.，Tran, L.-S.P.
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] ダイズの環境ストレス応答に関わるDREB1 転写因子の機能解析 (2014)
  • Author(s): 城所聡、渡邉慶太郎、大堀鉄平、森脇崇、圓山恭之進、Nang Myint Phyu Sin Htwe、藤田泰成、溝井順哉、篠崎一雄、篠崎和子
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] シロイヌナズナの4 個のAREB/ABF 転写因子が浸透圧ストレス時のアブシシン酸を介した遺伝子発現において中心的に機能している (2014)
  • Author(s): 吉田拓也、藤田泰成、圓山恭之進、篠崎一雄、篠崎和子
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] シロイヌナズナにおいてSnRK2 およびそれらの相互作用因子、SDB1 プロテインキナーゼが調節する新規な生理応答の発見 (2014)
  • Author(s): 最上惇郎、藤田泰成、吉田拓也、月居佳史、中神弘史、野村有子、藤原徹、西田翔、柳澤修一、石田哲也、森本恭子、城所聡、溝井順哉、篠崎一雄、篠崎和子
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] Identification of novel subclass III SnRK2-interacting proteins in Arabidopsis. (2013)
  • Author(s): Mogami, J., Fujita, Y., Kidokoro, S., Tsukiori, Y., Nakagami, H., Yanagisawa, S., Ishida, T., Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 4th International Conference on Arabidopsis Research
  • Place of Presentation: Sydney, Australia
• [Presentation] Promoter analysis of an Arabidopsis gene for 9-cis-epoxycartenoid dioxygenase-3 (AtNCED3) involved in dehydration-inducible transcription. (2013)
  • Author(s): Behnam, B., Iuchi, S., Fujita, M., Fujita, Y., Yamaguchi-Shinozaki, K., Kobayashi, M., Shinozaki, K.
  • Organizer: 24th International Conference on Arabidopsis Research
  • Place of Presentation: Sydney, Australia
• [Presentation] Functional analysis of ABF1 to reveal downstream transcription factors involved in SnRK2-mediated gene expression in ABA signalling. (2013)
  • Author(s): Yoshida, T., Fujita, Y., Maruyama, K., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 7th EPSO Conference
  • Place of Presentation: Porto Heli, Greece
• [Presentation] 国際研究機関と連携した乾燥耐性分子育種技術の開発 (2013)
  • Author(s): 藤田泰成
  • Organizer: 日本育種学会第124回講演会・第55回シンポジウム ワークショップ：気候変動に対応した21世紀育種戦略
  • Place of Presentation: 鹿児島
  • Invited
• [Presentation] A quartet of Arabidopsis AREB/ABF transcription factors play pivotal roles in gene expression via ABRE cis-elements in ABA signaling involved in drought stress tolerance. (2013)
  • Author(s): Yoshida, T., Fujita, Y., Maruyama, K., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: Keystone Symposia: Plant Abiotic Stress and Sustainable Agriculture: Translating Basic Understanding to Food Production
  • Place of Presentation: Taos, USA
• [Presentation] Identification of subclass III SnRK2-interacting proteins in Arabidopsis (2013)
  • Author(s): Mogami, J., Fujita, Y., Kidokoro, S., Tsukiori, Y., Nakagami, H., Nomura, Y., Yanagisawa, S., Ishida, T., Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: International Workshop on Plant Membrane Biology XVI
  • Place of Presentation: Kurashiki, Japan
• [Presentation] ダイズの乾燥ストレス応答性転写因子GmAREB3の機能解析. (2013)
  • Author(s): 月居佳史、藤田秦成、関田佐知子、Nang Myint Phyu Sin Htwe、吉田拓也、小平憲祐、城所聡、中島一雄、圓山恭之進、篠崎一雄、篠崎和子
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] 植物ポリアミントランスポーターを介したパラコート輸送機構 (2013)
  • Author(s): 藤田美紀、井内聖、山田晃嗣、藤田泰成、小林佑理子、小林正智、篠崎和子、篠崎一雄
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] イネの乾燥ストレス応答に関わる転写因子OsAREB8のX線結晶構造解析 (2013)
  • Author(s): 宮園健一、小浦翼、薛友林、窪田恵子、吉田拓也、藤田泰成、篠崎和子、田之倉優
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台
• [Presentation] AREB/ABF-SnRK2 pathway plays a key role in response to osmotic stress in plants. (2012)
  • Author(s): Fujita, Y., Yoshida, T., Nakashima, K., Nang, M.P.S.H., Sekita, S., Fujita, M., Todaka, D., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 23rd International Conference on Arabidopsis Research
  • Place of Presentation: Vienna, Austria
• [Presentation] Functional analysis of a bZIP-type transcription factor, ABF1, in SnRK2-mediated ABA signaling under drought stress. (2012)
  • Author(s): Yoshida, T., Fujita, Y., Maruyama, K., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 23rd International Conference on Arabidopsis Research
  • Place of Presentation: Vienna, Austria
• [Presentation] A rice PIF-like protein OsPIL1 functions as a key regulator of internode elongation and induces a morphological response to drought stress (2012)
  • Author(s): Todaka, D., Nakashima, K., Maruyama, K., Kidokoro, S., Osakabe, Y., Ito, Y., Matsukura, S., Fujita, Y., Yoshiwara, K., et al.
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju, Korea
• [Presentation] Identification of SnRK2-interacting proteins in Arabidopsis (2012)
  • Author(s): Mogami, J., Fujita, Y., Kidokoro, S., Tsukiori, Y., Nakagami, H., Nomura, Y., Yanagisawa, S., Ishida, T., Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju, Korea
• [Presentation] Natural variation in a polyamine transporter determines paraquat tolerance in Arabidopsis (2012)
  • Author(s): Fujita, M., Fujita, Y., Iuchi, S., Yamada, K., Kobayashi, Y., Urano, K., Kobayashi, M., Yamaguchi-Shinozaki, K., Shinozaki, K.
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju, Korea
• [Presentation] Agrobacterium-mediated transformation of soybean variated, BR16, Gene 35S:AREB1, analysis by Southern blotting. (2012)
  • Author(s): Girotto, L., Soldera, M.C.A., Marin, S.R.R., Kanamori, N., Fujita, Y., Yoshida, T., Marcelino-Guimarães, F.C., Yamaguchi-Shinozaki, K., Nepomuceno, A.L.
  • Organizer: 6th the International Crop Science Congress
  • Place of Presentation: Bento Gonçalves, Brazil
• [Book] Book title: Climate change and plant abiotic stress tolerance 執筆を担当したのは、全40chapterのうち、Chapter 20: Role of abscisic acid signaling in drought tolerance and preharvest sprouting under climate change (2014)
  • Author(s): Fujita, Y., Nakashima, K., Yoshida, T., Fujita, M., Shinozaki, K., Yamaguchi-Shinozaki, K. ( Tuteja, N., Gill, S.S. (eds.))
  • Publisher: Wiley Blackwell